Western blot abreview for Anti-BMP11 antibody [EPR4567(2)]

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse liver + kidney + heart & human Hela cells)
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
100 µg
Treatment
Halt Phosphatase inhibitor cocktail before lysis
Specification
Mouse liver + kidney + heart & human Hela cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Other product details

Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1x TBS + 0.5% milk + 0,2% Tween

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: Donkey Anti-Rabbit IgG, HRP conjugated
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
Pico Super signal chemiluminescent Subst
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 45, 12.5 kDa Non-specific: 37 kDa
Positive control
Vinculin

Additional data

Abcam response
We have not yet validated this antibody in mouse samples. This researcher has tested their mouse samples using our AbTrial program. If you are interested in testing this antibody in an untested species or application, please contact our Scientific Support Team.

Selina Moebus

Verified customer

Submitted May 28 2015

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