Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)]

Poor
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
liver
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 20% · Temperature: 20°C
Fixative
Formaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200

Secondary antibody

Dilution
1/700
Name
Non-Abcam antibody was used: biotinylated goat anti rabbit (VECTASTAIN)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Abcam response
Thank you for your review of IHC of paraffin-embedded mouse tissue, the first data of this kind we are aware of for this antibody. We recommend including a positive control of human tissue known to express either GDF8 or GDF11, such as skeletal muscle.

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Submitted Aug 13 2015

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