Anti-GDF15 antibody [6D12.H10.E4] (ab189358)
Key features and details
- Rat monoclonal [6D12.H10.E4] to GDF15
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-GDF15 antibody [6D12.H10.E4]
See all GDF15 primary antibodies -
Description
Rat monoclonal [6D12.H10.E4] to GDF15 -
Host species
Rat -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Chimpanzee -
Immunogen
Recombinant full length protein (MBP-tag) corresponding to Mouse GDF15 aa 1-303. Cross reactivity to MBP was removed via cross-adsorption chromatography.
Database link: Q9Z0J7 -
Positive control
- Human placenta tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 0.424% Potassium phosphate
Sterile filtered -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
Cross reactivity to MBP was removed via cross-adsorption chromatography. -
Clonality
Monoclonal -
Clone number
6D12.H10.E4 -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
Notes |
---|
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
Tissue specificity
Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 468770 Chimpanzee
- Entrez Gene: 9518 Human
- Entrez Gene: 29455 Rat
- Omim: 605312 Human
- SwissProt: Q99988 Human
- SwissProt: Q9Z0J6 Rat
- Unigene: 616962 Human
- Unigene: 44228 Rat
-
Alternative names
- GDF 15 antibody
- GDF-15 antibody
- Gdf15 antibody
see all
Images
Datasheets and documents
-
Datasheet download
References (6)
ab189358 has been referenced in 6 publications.
- Cao M et al. Elevated Expression of Growth Differentiation Factor-15 Is Associated With Acute Exacerbation of Idiopathic Pulmonary Fibrosis. Front Immunol 13:891448 (2022). PubMed: 35784345
- Weng JH et al. Colchicine acts selectively in the liver to induce hepatokines that inhibit myeloid cell activation. Nat Metab 3:513-522 (2021). PubMed: 33846641
- Qi P et al. Identification of growth differentiation factor 15 as a pro-fibrotic factor in mouse liver fibrosis progression. Int J Exp Pathol 102:148-156 (2021). PubMed: 33983642
- Li JJ et al. Growth Differentiation Factor 15 Maturation Requires Proteolytic Cleavage by PCSK3, -5, and -6. Mol Cell Biol 38:N/A (2018). PubMed: 30104250
- Hu P et al. Single-nucleus transcriptomic survey of cell diversity and functional maturation in postnatal mammalian hearts. Genes Dev 32:1344-1357 (2018). PubMed: 30254108
- Wang T et al. GDF15 is a heart-derived hormone that regulates body growth. EMBO Mol Med 9:1150-1164 (2017). WB, IHC-P ; Mouse . PubMed: 28572090