Anti-GDF15 antibody [7C12.B3.F2] (ab106112)
![Immunocytochemistry - Anti-GDF15 antibody [7C12.B3.F2] (ab106112)](https://www.abcam.com/ps/products/106/ab106112/Images/ab106112-206715-anti-gdf15-antibody-7c12b3f2-immunofluorescence.jpg "Immunocytochemistry - Anti-GDF15 antibody [7C12.B3.F2] (ab106112)")
Key features and details
- Mouse monoclonal [7C12.B3.F2] to GDF15
- Suitable for: ICC, WB
- Reacts with: Human, Recombinant fragment
- Isotype: unknown
Overview
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Product name
Anti-GDF15 antibody [7C12.B3.F2]
See all GDF15 primary antibodies -
Description
Mouse monoclonal [7C12.B3.F2] to GDF15 -
Host species
Mouse -
Specificity
ab106112 specifically reacts with an H variant sequence of GDF15 protein. -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Predicted to work with: Chimpanzee, Macaque monkey
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Immunogen
A synthetic peptide corresponding to a region near the amino terminal end of Human GDF15 protein. A residue of cysteine was added to facilitate coupling to KLH.
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Positive control
- Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant with SUMO expression tag.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab106112 was purified from concentrated tissue culture supernatant. It has been heated to 56°C for 30 minutes. -
Clonality
Monoclonal -
Clone number
7C12.B3.F2 -
Isotype
unknown -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| WB |
1/1000. Predicted molecular weight: 34 kDa.
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| Notes |
|---|
|
ICC
Use at an assay dependent concentration. |
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WB
1/1000. Predicted molecular weight: 34 kDa. |
Target
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Tissue specificity
Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 468770 Chimpanzee
- Entrez Gene: 9518 Human
- Omim: 605312 Human
- SwissProt: Q99988 Human
- Unigene: 616962 Human
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Alternative names
- GDF 15 antibody
- GDF-15 antibody
- Gdf15 antibody
see all
Images
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Immunocytochemistry - Anti-GDF15 antibody [7C12.B3.F2] (ab106112)This image is courtesy of Dr. Ruma Raha-Chowdhury (Cambridge University, United Kingdom)
ICC/IF image of GDF15 staining on human blood film using ab106112 (1:1000). The cells were fixed using formaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were then blocked using 10% donkey serum for 1 hour at 24°C. ab106112 was diluted to 1:1000 and incubated with cells for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to mouse IgG conjugated to Alexa Fluor 568.
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![Western blot - Anti-GDF15 antibody [7C12.B3.F2] (ab106112)](https://www.abcam.com/ps/products/106/ab106112/Images/ab106112-118452-anti-gdf15-antibody-7c12b3f2-western-blot.jpg)
Western blot - Anti-GDF15 antibody [7C12.B3.F2] (ab106112)All lanes : Anti-GDF15 antibody [7C12.B3.F2] (ab106112) at 1/1000 dilution
Lane 1 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant with SUMO expression tag
Lane 2 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 D variant with SUMO expression tag
Lane 3 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant
Lane 4 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 D variant
Secondary
All lanes : Peroxidase conjugated Gt-a-Mouse IgG at 1/40000 dilution
Performed under reducing conditions.
Predicted band size: 34 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab106112 has been referenced in 1 publication.
- Addison R et al. Therapeutic wavelengths of ultraviolet B radiation activate apoptotic, circadian rhythm, redox signalling and key canonical pathways in psoriatic epidermis. Redox Biol 41:101924 (2021). PubMed: 33812333