Anti-GDF15 antibody [7C12.B3.F2] (ab106112)

Mouse monoclonal GDF15 antibody [7C12.B3.F2]. Validated in WB, ELISA, ICC/IF and tested in Human. Independently reviewed in 1 review(s). Immunogen corresponding to synthetic peptide.

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Overview

  • Product name

    Anti-GDF15 antibody [7C12.B3.F2]
    See all GDF15 primary antibodies

  • Description

    Mouse monoclonal [7C12.B3.F2] to GDF15

  • Host species

    Mouse

  • Specificity

    ab106112 specifically reacts with an H variant sequence of GDF15 protein.

  • Tested applications

    Suitable for: ELISA, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Macaque monkey

  • Immunogen

    A synthetic peptide corresponding to a region near the amino terminal end of Human GDF15 protein. A residue of cysteine was added to facilitate coupling to KLH.

  • Positive control

    • Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant with SUMO expression tag.

Properties

Applications

Our Abpromise guarantee covers the use of ab106112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/150000.
WB 1/1000. Predicted molecular weight: 34 kDa.
ICC/IF 1/1000.

Target

  • Tissue specificity

    Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.

  • Sequence similarities

    Belongs to the TGF-beta family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • GDF 15 antibody
      • GDF-15 antibody
      • Gdf15 antibody
      • GDF15_HUMAN antibody
      • Growth differentiation factor 15 antibody
      • Growth/differentiation factor 15 antibody
      • Macrophage inhibitory cytokine 1 antibody
      • MIC 1 antibody
      • Mic-1 antibody
      • MIC1 antibody
      • NAG 1 antibody
      • NAG-1 antibody
      • NAG1 antibody
      • NRG 1 antibody
      • NRG-1 antibody
      • NRG1 antibody
      • NSAID (nonsteroidal anti inflammatory drug) activated protein 1 antibody
      • NSAID antibody
      • NSAID regulated protein 1 antibody
      • NSAID-activated gene 1 protein antibody
      • NSAID-regulated gene 1 protein antibody
      • PDF antibody
      • PLAB antibody
      • Placental bone morphogenetic protein antibody
      • Placental bone morphogenic protein antibody
      • Placental TGF beta antibody
      • Placental TGF-beta antibody
      • Prostate differentiation factor antibody
      • PTGF beta antibody
      • PTGFB antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-GDF15 antibody [7C12.B3.F2] (ab106112)
      Immunocytochemistry/ Immunofluorescence - Anti-GDF15 antibody [7C12.B3.F2] (ab106112)This image is courtesy of Dr. Ruma Raha-Chowdhury (Cambridge University, United Kingdom)

      ICC/IF image of GDF15 staining on human blood film using ab106112 (1:1000). The cells were fixed using formaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were then blocked using 10% donkey serum for 1 hour at 24°C. ab106112 was diluted to 1:1000 and incubated with cells for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to mouse IgG conjugated to Alexa Fluor 568.

    • Western blot - Anti-GDF15 antibody [7C12.B3.F2] (ab106112)
      Western blot - Anti-GDF15 antibody [7C12.B3.F2] (ab106112)
      All lanes : Anti-GDF15 antibody [7C12.B3.F2] (ab106112) at 1/1000 dilution

      Lane 1 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant with SUMO expression tag
      Lane 2 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 D variant with SUMO expression tag
      Lane 3 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant
      Lane 4 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 D variant

      Secondary
      All lanes : Peroxidase conjugated Gt-a-Mouse IgG at 1/40000 dilution

      Performed under reducing conditions.

      Predicted band size: 34 kDa

    References

    ab106112 has not yet been referenced specifically in any publications.

    Publishing research using ab106112? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-GDF15 antibody [7C12.B3.F2]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Human Cell (Human blood film)
    Specification
    Human blood film
    Permeabilization
    Yes - 0.1% TritonX in 0.1% PBS
    Fixative
    Formaldehyde
    Read More

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Sep 20 2013

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