Anti-GDF15 antibody [7C12.B3.F2] (ab106112)
Key features and details
- Mouse monoclonal [7C12.B3.F2] to GDF15
- Suitable for: ELISA, ICC, WB
- Reacts with: Human
- Isotype: unknown
Overview
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Product name
Anti-GDF15 antibody [7C12.B3.F2]
See all GDF15 primary antibodies -
Description
Mouse monoclonal [7C12.B3.F2] to GDF15 -
Host species
Mouse -
Specificity
ab106112 specifically reacts with an H variant sequence of GDF15 protein. -
Tested applications
Suitable for: ELISA, ICC, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Macaque monkey
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Immunogen
A synthetic peptide corresponding to a region near the amino terminal end of Human GDF15 protein. A residue of cysteine was added to facilitate coupling to KLH.
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Positive control
- Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant with SUMO expression tag.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab106112 was purified from concentrated tissue culture supernatant. It has been heated to 56°C for 30 minutes. -
Clonality
Monoclonal -
Clone number
7C12.B3.F2 -
Isotype
unknown -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab106112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/150000. | |
| ICC | Use at an assay dependent concentration. | |
| WB | 1/1000. Predicted molecular weight: 34 kDa. |
Target
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Tissue specificity
Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 468770 Chimpanzee
- Entrez Gene: 9518 Human
- Omim: 605312 Human
- SwissProt: Q99988 Human
- Unigene: 616962 Human
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Alternative names
- GDF 15 antibody
- GDF-15 antibody
- Gdf15 antibody
see all
Images
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![Immunocytochemistry - Anti-GDF15 antibody [7C12.B3.F2] (ab106112)](https://www.abcam.com/ps/products/106/ab106112/Images/ab106112-206715-anti-gdf15-antibody-7c12b3f2-immunofluorescence.jpg)
Immunocytochemistry - Anti-GDF15 antibody [7C12.B3.F2] (ab106112)This image is courtesy of Dr. Ruma Raha-Chowdhury (Cambridge University, United Kingdom)ICC/IF image of GDF15 staining on human blood film using ab106112 (1:1000). The cells were fixed using formaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were then blocked using 10% donkey serum for 1 hour at 24°C. ab106112 was diluted to 1:1000 and incubated with cells for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to mouse IgG conjugated to Alexa Fluor 568.
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![Western blot - Anti-GDF15 antibody [7C12.B3.F2] (ab106112)](https://www.abcam.com/ps/products/106/ab106112/Images/ab106112-118452-anti-gdf15-antibody-7c12b3f2-western-blot.jpg)
Western blot - Anti-GDF15 antibody [7C12.B3.F2] (ab106112)All lanes : Anti-GDF15 antibody [7C12.B3.F2] (ab106112) at 1/1000 dilution
Lane 1 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant with SUMO expression tag
Lane 2 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 D variant with SUMO expression tag
Lane 3 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant
Lane 4 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 D variant
Secondary
All lanes : Peroxidase conjugated Gt-a-Mouse IgG at 1/40000 dilution
Performed under reducing conditions.
Predicted band size: 34 kDa
Protocols
Datasheets and documents
References (0)
ab106112 has not yet been referenced specifically in any publications.
