Key features and details
- Mouse monoclonal [7C12.B3.F2] to GDF15
- Suitable for: ELISA, ICC, WB
- Reacts with: Human
- Isotype: unknown
Product nameAnti-GDF15 antibody [7C12.B3.F2]
See all GDF15 primary antibodies
DescriptionMouse monoclonal [7C12.B3.F2] to GDF15
Specificityab106112 specifically reacts with an H variant sequence of GDF15 protein.
Tested applicationsSuitable for: ELISA, ICC, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Macaque monkey
A synthetic peptide corresponding to a region near the amino terminal end of Human GDF15 protein. A residue of cysteine was added to facilitate coupling to KLH.
- Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant with SUMO expression tag.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
Concentration information loading...
PurityProtein A purified
Purification notesab106112 was purified from concentrated tissue culture supernatant. It has been heated to 56°C for 30 minutes.
Our Abpromise guarantee covers the use of ab106112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|WB||1/1000. Predicted molecular weight: 34 kDa.|
Tissue specificityHighly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.
Sequence similaritiesBelongs to the TGF-beta family.
- Information by UniProt
- GDF 15 antibody
- GDF-15 antibody
- Gdf15 antibody
ICC/IF image of GDF15 staining on human blood film using ab106112 (1:1000). The cells were fixed using formaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were then blocked using 10% donkey serum for 1 hour at 24°C. ab106112 was diluted to 1:1000 and incubated with cells for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to mouse IgG conjugated to Alexa Fluor 568.
All lanes : Anti-GDF15 antibody [7C12.B3.F2] (ab106112) at 1/1000 dilution
Lane 1 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant with SUMO expression tag
Lane 2 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 D variant with SUMO expression tag
Lane 3 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant
Lane 4 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 D variant
All lanes : Peroxidase conjugated Gt-a-Mouse IgG at 1/40000 dilution
Performed under reducing conditions.
Predicted band size: 34 kDa
ab106112 has not yet been referenced specifically in any publications.