Product nameAnti-GDF15 antibody [7C12.B3.F2]
See all GDF15 primary antibodies
DescriptionMouse monoclonal [7C12.B3.F2] to GDF15
Specificityab106112 specifically reacts with an H variant sequence of GDF15 protein.
Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Macaque monkey
A synthetic peptide corresponding to a region near the amino terminal end of Human GDF15 protein. A residue of cysteine was added to facilitate coupling to KLH.
- Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant with SUMO expression tag.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
Concentration information loading...
PurityProtein A purified
Purification notesab106112 was purified from concentrated tissue culture supernatant. It has been heated to 56°C for 30 minutes.
Our Abpromise guarantee covers the use of ab106112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 34 kDa.|
Tissue specificityHighly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.
Sequence similaritiesBelongs to the TGF-beta family.
- Information by UniProt
- GDF 15 antibody
- GDF-15 antibody
- Gdf15 antibody
ICC/IF image of GDF15 staining on human blood film using ab106112 (1:1000). The cells were fixed using formaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were then blocked using 10% donkey serum for 1 hour at 24°C. ab106112 was diluted to 1:1000 and incubated with cells for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to mouse IgG conjugated to Alexa Fluor 568.
All lanes : Anti-GDF15 antibody [7C12.B3.F2] (ab106112) at 1/1000 dilution
Lane 1 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant with SUMO expression tag
Lane 2 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 D variant with SUMO expression tag
Lane 3 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant
Lane 4 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 D variant
All lanes : Peroxidase conjugated Gt-a-Mouse IgG at 1/40000 dilution
Performed under reducing conditions.
Predicted band size: 34 kDa
ab106112 has not yet been referenced specifically in any publications.