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    gdf15-antibody-ab105738.pdf

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Cell Biology Cell Cycle Cell Division Cytokinesis
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Anti-GDF15 antibody (ab105738)

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Western blot - Anti-GDF15 antibody (ab105738)

    Key features and details

    • Rabbit polyclonal to GDF15
    • Suitable for: WB
    • Reacts with: Mouse, Chinese hamster, Recombinant fragment
    • Isotype: IgG

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    Recombinant human GDF15 protein (ab125769)
    Protein
    Product image
    Recombinant Mouse GDF15 protein (denatured) (ab202199)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-GDF15 antibody
      See all GDF15 primary antibodies
    • Description

      Rabbit polyclonal to GDF15
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Mouse
      Chinese hamster
      Recombinant fragment
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to amino acids near the C-terminal of Mouse GDF15.

    • General notes

      Expiration date is one (1) year from date of opening product.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
    • Concentration information loading...
    • Purity

      Protein A purified
    • Purification notes

      ab105738 is affinity purified from monospecific antiserum by immunoaffinity chromatography.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Cell Cycle
      • Cell Division
      • Cytokinesis
      • Signal Transduction
      • Growth Factors/Hormones
      • TGF
      • Signal Transduction
      • Growth Factors/Hormones
      • Hormones
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • Structures
      • Bone
      • Cardiovascular
      • Heart
      • Hypertrophy
      • Hormones

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Mouse GDF15 protein (denatured) (ab202199)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab105738 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Mouse
    Chinese hamster
    Recombinant fragment
    All applications
    Rat
    Cow
    Dog
    Pig
    Application Abreviews Notes
    WB
    1/3000 - 1/7000. Predicted molecular weight: 33 kDa.
    Notes
    WB
    1/3000 - 1/7000. Predicted molecular weight: 33 kDa.

    Target

    • Tissue specificity

      Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.
    • Sequence similarities

      Belongs to the TGF-beta family.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession Q99988 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 618677 Cow
      • Entrez Gene: 23886 Mouse
      • Entrez Gene: 29455 Rat
      • SwissProt: Q9Z0J7 Mouse
      • SwissProt: Q9Z0J6 Rat
      • Unigene: 31325 Mouse
      • Unigene: 44228 Rat
      • Alternative names

        • GDF 15 antibody
        • GDF-15 antibody
        • Gdf15 antibody
        • GDF15_HUMAN antibody
        • Growth differentiation factor 15 antibody
        • Growth/differentiation factor 15 antibody
        • Macrophage inhibitory cytokine 1 antibody
        • MIC 1 antibody
        • Mic-1 antibody
        • MIC1 antibody
        • NAG 1 antibody
        • NAG-1 antibody
        • NAG1 antibody
        • NRG 1 antibody
        • NRG-1 antibody
        • NRG1 antibody
        • NSAID (nonsteroidal anti inflammatory drug) activated protein 1 antibody
        • NSAID antibody
        • NSAID regulated protein 1 antibody
        • NSAID-activated gene 1 protein antibody
        • NSAID-regulated gene 1 protein antibody
        • PDF antibody
        • PLAB antibody
        • Placental bone morphogenetic protein antibody
        • Placental bone morphogenic protein antibody
        • Placental TGF beta antibody
        • Placental TGF-beta antibody
        • Prostate differentiation factor antibody
        • PTGF beta antibody
        • PTGFB antibody
        see all

      Images

      • Western blot - Anti-GDF15 antibody (ab105738)
        Western blot - Anti-GDF15 antibody (ab105738)
        All lanes : Anti-GDF15 antibody (ab105738) at 1/1000 dilution

        Lane 1 : Recombinant MBP-GDF15 fusion protein purified from E.coli
        Lane 2 : Yeast cell lysate expressing SUMO-Mouse GDF15
        Lane 3 : Human GDF15 monomer purified from CHO-K1 cells

        Secondary
        All lanes : Goat-anti-Rabbit HRP at 1/40000 dilution

        Performed under reducing conditions.

        Predicted band size: 33 kDa



        Expect bands in Western blots of approximately 14 and 28 kDa in size corresponding to GDF15 monomer and dimer, respectively.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab105738? Please let us know so that we can cite the reference in this datasheet.

      ab105738 has not yet been referenced specifically in any publications.

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