Anti-GDF15 antibody (ab105738)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-GDF15 antibody
See all GDF15 primary antibodies -
Description
Rabbit polyclonal to GDF15 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Sandwich ELISAmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Pig
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Immunogen
Synthetic peptide corresponding to amino acids near the C-terminal of Mouse GDF15.
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Positive control
- Recombinant MBP-GDF15 fusion protein; Yeast cell lysate expressing SUMO-mouse GDF15; Human GDF15 monomer.
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General notes
Expiration date is one (1) year from date of opening product.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab105738 is affinity purified from monospecific antiserum by immunoaffinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab105738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/3000 - 1/7000. Predicted molecular weight: 33 kDa. | |
| Sandwich ELISA | 1/100000 - 1/120000. |
Target
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Tissue specificity
Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 618677 Cow
- Entrez Gene: 9518 Human
- Entrez Gene: 23886 Mouse
- Entrez Gene: 29455 Rat
- Omim: 605312 Human
- SwissProt: Q99988 Human
- SwissProt: Q9Z0J7 Mouse
- SwissProt: Q9Z0J6 Rat
see all -
Alternative names
- GDF 15 antibody
- GDF-15 antibody
- Gdf15 antibody
see all
Images
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Western blot - Anti-GDF15 antibody (ab105738)All lanes : Anti-GDF15 antibody (ab105738) at 1/1000 dilution
Lane 1 : Recombinant MBP-GDF15 fusion protein purified from E.coli
Lane 2 : Yeast cell lysate expressing SUMO-Mouse GDF15
Lane 3 : Human GDF15 monomer purified from CHO-K1 cells
Secondary
All lanes : Goat-anti-Rabbit HRP at 1/40000 dilution
Performed under reducing conditions.
Predicted band size: 33 kDa
Expect bands in Western blots of approximately 14 and 28 kDa in size corresponding to GDF15 monomer and dimer, respectively.
Protocols
References
ab105738 has not yet been referenced specifically in any publications.
