Anti-GDF15 antibody (ab39999)
Key features and details
- Goat polyclonal to GDF15
- Suitable for: ICC, WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GDF15 antibody
See all GDF15 primary antibodies -
Description
Goat polyclonal to GDF15 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide: QKTDTGVSLQTYDD, corresponding to C terminal amino acids 286-299 of Human GDF15
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab39999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| WB |
Human
|
| All applications |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
|
|
| WB | (2) |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 30-35 kDa (predicted molecular weight: 34 kDa).
1 hour primary incubation is recommended for this product. |
| IHC-P | (1) |
Use a concentration of 5 µg/ml.
|
| Flow Cyt |
Use a concentration of 10 µg/ml.
|
| Notes |
|---|
|
ICC
Use at an assay dependent concentration. |
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 30-35 kDa (predicted molecular weight: 34 kDa). 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 5 µg/ml. |
|
Flow Cyt
Use a concentration of 10 µg/ml. |
Target
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Tissue specificity
Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 9518 Human
- Entrez Gene: 23886 Mouse
- Omim: 605312 Human
- SwissProt: Q99988 Human
- SwissProt: Q9Z0J7 Mouse
- Unigene: 616962 Human
- Unigene: 31325 Mouse
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Alternative names
- GDF 15 antibody
- GDF-15 antibody
- Gdf15 antibody
see all
Images
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Western blot - Anti-GDF15 antibody (ab39999)All lanes : Anti-GDF15 antibody (ab39999) at 0.3 µg/ml
Lane 1 : Human Testes whole cell lysate in RIPA buffer
Lane 2 : Human Skin whole cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 34 kDa
Observed band size: 30-35 kDa why is the actual band size different from the predicted? -
Immunocytochemistry - Anti-GDF15 antibody (ab39999)This image is courtesy of an abreview submitted by Dr. Ruma Raha-Chowdhury, Cambridge University, United Kingdom.ICC/IF image of human bone marrow smear stained with Ab39999(1/250). The smear was permeabilized in 0.1% PBS-Triton X for 1h and incubated with 10% donkey serum for 1hr at 24°C to block non-specific protein-protein interactions. The sections were then incubated with ab39999 used at 1/250 dilution, overnight at +4°C. The secondary antibody was Alexa Fluor® 568 donkey anti-goat used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Flow Cytometry - Anti-GDF15 antibody (ab39999)Flow cytometric analysis of paraformaldehyde fixed, 0.5% Triton permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling GDF-15 using ab39999 at 10 μg/ml followed by Alexa Fluor 488 secondary antibody (blue) at 1 μg/ml. Unimmunized goat IgG was used as isotype control, followed by Alexa Fluor 488 secondary antibody (black).
Protocols
Datasheets and documents
References (6)
ab39999 has been referenced in 6 publications.
- Xiang Y et al. In vitro expansion affects the response of human bone marrow stromal cells to irradiation. Stem Cell Res Ther 10:82 (2019). PubMed: 30850008
- Gabriel KN et al. Association and regulation of protein factors of field effect in prostate tissues. Int J Oncol 49:1541-1552 (2016). ICC/IF ; Human . PubMed: 27634112
- Zhou YM et al. Growth differentiation factor-15 (GDF-15), novel biomarker for assessing atrial fibrosis in patients with atrial fibrillation and rheumatic heart disease. Int J Clin Exp Med 8:21201-7 (2015). PubMed: 26885055
- Jones AC et al. Prostate field cancerization: deregulated expression of macrophage inhibitory cytokine 1 (MIC-1) and platelet derived growth factor A (PDGF-A) in tumor adjacent tissue. PLoS One 10:e0119314 (2015). IHC-P ; Human . PubMed: 25767870
- Hegde NV et al. Interrelationships between tissue iron status and erythropoiesis during postweaning development following neonatal iron deficiency in rats. Am J Physiol Gastrointest Liver Physiol 300:G470-6 (2011). WB ; Rat . PubMed: 21193529
- Chenau J et al. The cell line secretome, a suitable tool for investigating proteins released in vivo by tumors: application to the study of p53-modulated proteins secreted in lung cancer cells. J Proteome Res 8:4579-91 (2009). WB ; Human . PubMed: 19639960
