Product nameAnti-GDF15 antibody
See all GDF15 primary antibodies
DescriptionGoat polyclonal to GDF15
Tested applicationsSuitable for: WB, ICC/IF, IHC-Fr, IHC-P, Flow Cytmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
- WB: Human testes whole cell lysate. IHC-P: Human prostate and placenta tissue. ICC/IF: Human bone marrow smear tissue. Flow: HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab39999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 30-35 kDa (predicted molecular weight: 34 kDa).|
|IHC-Fr||1/200. See Abreview.|
|IHC-P||Use a concentration of 5 µg/ml.|
|Flow Cyt||Use a concentration of 10 µg/ml.|
Tissue specificityHighly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.
Sequence similaritiesBelongs to the TGF-beta family.
- Information by UniProt
- GDF 15 antibody
- GDF-15 antibody
- Gdf15 antibody
All lanes : Anti-GDF15 antibody (ab39999) at 0.3 µg/ml
Lane 1 : Human Testes whole cell lysate in RIPA buffer
Lane 2 : Human Skin whole cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 34 kDa
Observed band size: 30-35 kDa why is the actual band size different from the predicted?
ICC/IF image of human bone marrow smear stained with Ab39999(1/250). The smear was permeabilized in 0.1% PBS-Triton X for 1h and incubated with 10% donkey serum for 1hr at 24°C to block non-specific protein-protein interactions. The sections were then incubated with ab39999 used at 1/250 dilution, overnight at +4°C. The secondary antibody was Alexa Fluor® 568 donkey anti-goat used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Flow cytometric analysis of paraformaldehyde fixed, 0.5% Triton permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling GDF-15 using ab39999 at 10 μg/ml followed by Alexa Fluor 488 secondary antibody (blue) at 1 μg/ml. Unimmunized goat IgG was used as isotype control, followed by Alexa Fluor 288 secondary antibody (black).
This product has been referenced in:
- Xiang Y et al. In vitro expansion affects the response of human bone marrow stromal cells to irradiation. Stem Cell Res Ther 10:82 (2019). Read more (PubMed: 30850008) »
- Gabriel KN et al. Association and regulation of protein factors of field effect in prostate tissues. Int J Oncol 49:1541-1552 (2016). ICC/IF ; Human . Read more (PubMed: 27634112) »