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    gdf15-antibody-ab39999.pdf

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Cell Biology Cell Cycle Cell Division Cytokinesis
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Anti-GDF15 antibody (ab39999)

  • Datasheet
Reviews (5) Submit a question References (6)

Product price, shipping and contact information

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Western blot - Anti-GDF15 antibody (ab39999)
  • Immunocytochemistry - Anti-GDF15 antibody (ab39999)
  • Flow Cytometry - Anti-GDF15 antibody (ab39999)

Key features and details

  • Goat polyclonal to GDF15
  • Suitable for: ICC, WB, IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-GDF15 antibody
    See all GDF15 primary antibodies
  • Description

    Goat polyclonal to GDF15
  • Host species

    Goat
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide: QKTDTGVSLQTYDD, corresponding to C terminal amino acids 286-299 of Human GDF15

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Cytokinesis
    • Signal Transduction
    • Growth Factors/Hormones
    • TGF
    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Bone
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Hormones

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Positive Controls

    • Recombinant human GDF15 protein (ab125769)
    • Recombinant human GDF15 (mutated H202D) protein (ab125826)
  • Recombinant Protein

    • Recombinant Mouse GDF15 protein (denatured) (ab202199)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab39999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
WB
Human
All applications
Mouse
Application Abreviews Notes
ICC
Use at an assay dependent concentration.
WB (2)
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 30-35 kDa (predicted molecular weight: 34 kDa).

1 hour primary incubation is recommended for this product.

IHC-P (1)
Use a concentration of 5 µg/ml.
Flow Cyt
Use a concentration of 10 µg/ml.
Notes
ICC
Use at an assay dependent concentration.
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 30-35 kDa (predicted molecular weight: 34 kDa).

1 hour primary incubation is recommended for this product.

IHC-P
Use a concentration of 5 µg/ml.
Flow Cyt
Use a concentration of 10 µg/ml.

Target

  • Tissue specificity

    Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.
  • Sequence similarities

    Belongs to the TGF-beta family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q99988 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9518 Human
    • Entrez Gene: 23886 Mouse
    • Omim: 605312 Human
    • SwissProt: Q99988 Human
    • SwissProt: Q9Z0J7 Mouse
    • Unigene: 616962 Human
    • Unigene: 31325 Mouse
    • Alternative names

      • GDF 15 antibody
      • GDF-15 antibody
      • Gdf15 antibody
      • GDF15_HUMAN antibody
      • Growth differentiation factor 15 antibody
      • Growth/differentiation factor 15 antibody
      • Macrophage inhibitory cytokine 1 antibody
      • MIC 1 antibody
      • Mic-1 antibody
      • MIC1 antibody
      • NAG 1 antibody
      • NAG-1 antibody
      • NAG1 antibody
      • NRG 1 antibody
      • NRG-1 antibody
      • NRG1 antibody
      • NSAID (nonsteroidal anti inflammatory drug) activated protein 1 antibody
      • NSAID antibody
      • NSAID regulated protein 1 antibody
      • NSAID-activated gene 1 protein antibody
      • NSAID-regulated gene 1 protein antibody
      • PDF antibody
      • PLAB antibody
      • Placental bone morphogenetic protein antibody
      • Placental bone morphogenic protein antibody
      • Placental TGF beta antibody
      • Placental TGF-beta antibody
      • Prostate differentiation factor antibody
      • PTGF beta antibody
      • PTGFB antibody
      see all

    Images

    • Western blot - Anti-GDF15 antibody (ab39999)
      Western blot - Anti-GDF15 antibody (ab39999)
      All lanes : Anti-GDF15 antibody (ab39999) at 0.3 µg/ml

      Lane 1 : Human Testes whole cell lysate in RIPA buffer
      Lane 2 : Human Skin whole cell lysate in RIPA buffer

      Lysates/proteins at 35 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 34 kDa
      Observed band size: 30-35 kDa
      why is the actual band size different from the predicted?

    • Immunocytochemistry - Anti-GDF15 antibody (ab39999)
      Immunocytochemistry - Anti-GDF15 antibody (ab39999)This image is courtesy of an abreview submitted by Dr. Ruma Raha-Chowdhury, Cambridge University, United Kingdom.

      ICC/IF image of human bone marrow smear stained with Ab39999(1/250). The smear was permeabilized in 0.1% PBS-Triton X for 1h and incubated with 10% donkey serum for 1hr at 24°C to block non-specific protein-protein interactions. The sections were then incubated with ab39999 used at 1/250 dilution, overnight at +4°C. The secondary antibody was Alexa Fluor® 568 donkey anti-goat used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      See Abreview

    • Flow Cytometry - Anti-GDF15 antibody (ab39999)
      Flow Cytometry - Anti-GDF15 antibody (ab39999)

      Flow cytometric analysis of paraformaldehyde fixed, 0.5% Triton permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling GDF-15 using ab39999 at 10 μg/ml followed by Alexa Fluor 488 secondary antibody (blue) at 1 μg/ml. Unimmunized goat IgG was used as isotype control, followed by Alexa Fluor 488 secondary antibody (black).

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (6)

    Publishing research using ab39999? Please let us know so that we can cite the reference in this datasheet.

    ab39999 has been referenced in 6 publications.

    • Xiang Y  et al. In vitro expansion affects the response of human bone marrow stromal cells to irradiation. Stem Cell Res Ther 10:82 (2019). PubMed: 30850008
    • Gabriel KN  et al. Association and regulation of protein factors of field effect in prostate tissues. Int J Oncol 49:1541-1552 (2016). ICC/IF ; Human . PubMed: 27634112
    • Zhou YM  et al. Growth differentiation factor-15 (GDF-15), novel biomarker for assessing atrial fibrosis in patients with atrial fibrillation and rheumatic heart disease. Int J Clin Exp Med 8:21201-7 (2015). PubMed: 26885055
    • Jones AC  et al. Prostate field cancerization: deregulated expression of macrophage inhibitory cytokine 1 (MIC-1) and platelet derived growth factor A (PDGF-A) in tumor adjacent tissue. PLoS One 10:e0119314 (2015). IHC-P ; Human . PubMed: 25767870
    • Hegde NV  et al. Interrelationships between tissue iron status and erythropoiesis during postweaning development following neonatal iron deficiency in rats. Am J Physiol Gastrointest Liver Physiol 300:G470-6 (2011). WB ; Rat . PubMed: 21193529
    • Chenau J  et al. The cell line secretome, a suitable tool for investigating proteins released in vivo by tumors: application to the study of p53-modulated proteins secreted in lung cancer cells. J Proteome Res 8:4579-91 (2009). WB ; Human . PubMed: 19639960

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GDF15 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (human colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mm sodium citrate
    Permeabilization
    No
    Specification
    human colon
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 16 2017

    Western blot abreview for Anti-GDF15 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Adipose and MEFs)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    25 µg
    Treatment
    Epididymal Fast/Refed and MEFs with Tunicamycin (6h)
    Specification
    Adipose and MEFs
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Satish Patel

    Verified customer

    Submitted Aug 01 2017

    Western blot abreview for Anti-GDF15 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (15% gel)
    Sample
    Human Cell lysate - whole cell (HELA)
    Specification
    HELA
    Treatment
    10uM Tunicamycin for 24hrs
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 30 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GDF15 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Human Cell (Bone Marrow)
    Specification
    Bone Marrow
    Permeabilization
    Yes - 0.1% TritonX in 0.1% PBS
    Fixative
    Paraformaldehyde
    Read More

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Aug 06 2013

    Immunohistochemistry (Frozen sections) abreview for Anti-GDF15 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (aortic plaque)
    Specification
    aortic plaque
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 20°C
    Read More

    Miss. Silke Vorwald

    Verified customer

    Submitted Jun 04 2008

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