Anti-GDF15 antibody (ab39999)
- Datasheet
- References (6)
- Protocols
Overview
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Product nameAnti-GDF15 antibody
See all GDF15 primary antibodies -
DescriptionGoat polyclonal to GDF15
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Host speciesGoat
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Tested applicationsSuitable for: WB, ICC/IF, IHC-Fr, IHC-P, Flow Cytmore details
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Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide: QKTDTGVSLQTYDD, corresponding to C terminal amino acids 286-299 of Human GDF15
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Positive control
- WB: Recombinant human GDF15 (mutated H202 D) protein (ab125826), Human testis, skin and prostate lysate; Lysate prepared from transfected HEK293 cells transiently expressing GDF15. IHC-P: Human prostate and placenta tissue. ICC/IF: Human bone marrow smear tissue. Flow: HeLa cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab39999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa). | |
| ICC/IF | 1/250. | |
| IHC-Fr | 1/200. See Abreview. | |
| IHC-P | Use a concentration of 5 µg/ml. | |
| Flow Cyt | Use a concentration of 10 µg/ml. |
Target
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Tissue specificityHighly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.
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Sequence similaritiesBelongs to the TGF-beta family.
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Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 9518 Human
- Entrez Gene: 23886 Mouse
- Entrez Gene: 29455 Rat
- Omim: 605312 Human
- SwissProt: Q99988 Human
- SwissProt: Q9Z0J7 Mouse
- SwissProt: Q9Z0J6 Rat
- Unigene: 616962 Human
see all -
Alternative names
- GDF 15 antibody
- GDF-15 antibody
- Gdf15 antibody
see all
Images
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Western blot - Anti-GDF15 antibody (ab39999)All lanes : Anti-GDF15 antibody (ab39999) at 0.3 µg/ml
Lane 1 : Human testis lysate
Lane 2 : Human skin lysate
Predicted band size: 34 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF15 antibody (ab39999)Paraffin-embedded human placenta tissue stained for GDF-15 using ab39999 at 5 μg/ml in immunohistochemical analysis. Steamed antigen retrieval with citrate buffer pH 6.
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Immunocytochemistry/ Immunofluorescence - Anti-GDF15 antibody (ab39999)This image is courtesy of an abreview submitted by Dr. Ruma Raha-Chowdhury, Cambridge University, United Kingdom.ICC/IF image of human bone marrow smear stained with Ab39999(1/250). The smear was permeabilized in 0.1% PBS-Triton X for 1h and incubated with 10% donkey serum for 1hr at 24°C to block non-specific protein-protein interactions. The sections were then incubated with ab39999 used at 1/250 dilution, overnight at +4°C. The secondary antibody was Alexa Fluor® 568 donkey anti-goat used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF15 antibody (ab39999)Paraffin-embedded human prostate tissue stained for GDF-15 using ab39999 at 5 μg/ml in immunohistochemical analysis. Steamed antigen retrieval with citrate buffer pH 6.
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Flow Cytometry - Anti-GDF15 antibody (ab39999)Flow cytometric analysis of paraformaldehyde fixed, 0.5% Triton permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling GDF-15 using ab39999 at 10 μg/ml followed by Alexa Fluor 488 secondary antibody (blue) at 1 μg/ml. Unimmunized goat IgG was used as isotype control, followed by Alexa Fluor 288 secondary antibody (black).
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Western blot - Anti-GDF15 antibody (ab39999)Anti-GDF15 antibody (ab39999) at 0.3 µg/ml + Human Prostate lysate in RIPA buffer at 35 µg
Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence. -
Western blot - Anti-GDF15 antibody (ab39999)Anti-GDF15 antibody (ab39999) at 0.3 µg/ml + lysate prepared from transfected HEK293 cells transiently expressing GDF15
Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References
This product has been referenced in:
- Xiang Y et al. In vitro expansion affects the response of human bone marrow stromal cells to irradiation. Stem Cell Res Ther 10:82 (2019). Read more (PubMed: 30850008) »
- Gabriel KN et al. Association and regulation of protein factors of field effect in prostate tissues. Int J Oncol 49:1541-1552 (2016). ICC/IF ; Human . Read more (PubMed: 27634112) »
