Key features and details
- Rabbit polyclonal to GDF15
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GDF15 antibody
See all GDF15 primary antibodies
DescriptionRabbit polyclonal to GDF15
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human GDF15 aa 250 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
- Recombinant human GDF15 protein (ab125769) can be used as a positive control in WB. This antibody gave a positive signal in the following human tissue lysates: Prostate; Colon.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab82569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).|
Tissue specificityHighly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.
Sequence similaritiesBelongs to the TGF-beta family.
- Information by UniProt
- GDF 15 antibody
- GDF-15 antibody
- Gdf15 antibody
All lanes : Anti-GDF15 antibody (ab82569) at 1 µg/ml
Lane 1 : Prostate (Human) Cytoplasmic Lysate - adult normal tissue (ab30294)
Lane 2 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg/ml per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Additional bands at: 53 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
ICC/IF image of ab82569 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82569, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab82569 has been referenced in 1 publication.
- Tang X et al. Elevated growth differentiating factor 15 expression predicts long-term benefit of docetaxel, cisplatin and 5-fluorouracil induction chemotherapy in patients with oral cancer. Oncol Lett 15:8118-8124 (2018). PubMed: 29731919