Overview

  • Product name

    Anti-GDF15 antibody [EPR19939]
    See all GDF15 primary antibodies
  • Description

    Rabbit monoclonal [EPR19939] to GDF15
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human GDF15 aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: Q99988

  • Positive control

    • WB: HepG2 and LNCaP whole cell lysates; human prostate cancer and placenta lysates; untreated HT1080 and treated with 200ng/ml Phorbol-12-myristate-13-acetate (TPA) for 24 hours whole cell lysates. IHC-P: Human placenta and prostate hyperplasia tissues. IP: HepG2 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab206414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC tests show positive staining only on human placenta and prostate hyperplasia tissues, other tissues tested were negative.

WB 1/1000. Detects a band of approximately 35, 12.5 kDa (predicted molecular weight: 34 kDa).
IP 1/30.

Target

  • Tissue specificity

    Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.
  • Sequence similarities

    Belongs to the TGF-beta family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • GDF 15 antibody
    • GDF-15 antibody
    • Gdf15 antibody
    • GDF15_HUMAN antibody
    • Growth differentiation factor 15 antibody
    • Growth/differentiation factor 15 antibody
    • Macrophage inhibitory cytokine 1 antibody
    • MIC 1 antibody
    • Mic-1 antibody
    • MIC1 antibody
    • NAG 1 antibody
    • NAG-1 antibody
    • NAG1 antibody
    • NRG 1 antibody
    • NRG-1 antibody
    • NRG1 antibody
    • NSAID (nonsteroidal anti inflammatory drug) activated protein 1 antibody
    • NSAID antibody
    • NSAID regulated protein 1 antibody
    • NSAID-activated gene 1 protein antibody
    • NSAID-regulated gene 1 protein antibody
    • PDF antibody
    • PLAB antibody
    • Placental bone morphogenetic protein antibody
    • Placental bone morphogenic protein antibody
    • Placental TGF beta antibody
    • Placental TGF-beta antibody
    • Prostate differentiation factor antibody
    • PTGF beta antibody
    • PTGFB antibody
    see all

Images

  • All lanes : Anti-GDF15 antibody [EPR19939] (ab206414) at 1/1000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : LNCaP (Human prostate cancer cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 34 kDa
    Observed band size: 12.5,35 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 minutes; Lane 2: 10 seconds.

    The molecular weight observed is consistent with the literature (PMID: 20884666).

  • All lanes : Anti-GDF15 antibody [EPR19939] (ab206414) at 1/1000 dilution

    Lane 1 : Human prostate cancer lysate
    Lane 2 : Human placenta lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 34 kDa
    Observed band size: 12.5 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 10 seconds; Lane 2: 3 minutes.

    The molecular weight observed is consistent with the literature (PMID: 20884666).

  • All lanes : Anti-GDF15 antibody [EPR19939] (ab206414) at 1/1000 dilution

    Lane 1 : Untreated HT1080 (Human fibrosarcoma cell line) whole cell lysate
    Lane 2 : HT1080 (Human fibrosarcoma cell line) whole cell lysate treated with 200ng/ml Phorbol-12-myristate-13-acetate (TPA) for 24 hours

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 34 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression level of GDF15 can be increased by TPA treatment (PMID: 15757899).

  • All lanes : Anti-GDF15 antibody [EPR19939] (ab206414) at 1/1000 dilution

    Lane 1 : Untreated HT1080 (Human fibrosarcoma cell line) whole cell lysate
    Lane 2 : HT1080 (Human fibrosarcoma cell line) whole cell lysate treated with 200ng/ml Phorbol-12-myristate-13-acetate (TPA) for 24 hours

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 34 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Both pro and mature form can be increased by TPA treatment.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling GDF15 with ab206414 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. 

    Cytoplasmic staining on human placenta is observed (PMID: 9593718). Positive staining on human placenta and prostate hyperplasia, other tissues tested were negative.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue labeling GDF15 with ab206414 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. 

    Cytoplasmic staining on human prostate hyperplasia is observed (PMID: 9593718). Positive staining on human placenta and prostate hyperplasia, other tissues tested were negative.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • GDF15 was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab206414 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab206414 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: HepG2 whole cell lysate 10µg (Input).

    Lane 2: ab206414 IP in HepG2 whole cell lysate.

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab206414 in HepG2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

References

This product has been referenced in:

  • Duan L  et al. The role of GDF15 in bone metastasis of lung adenocarcinoma cells. Oncol Rep 41:2379-2388 (2019). Read more (PubMed: 30816507) »
  • Jiang G  et al. IL-17A and GDF15 are able to induce epithelial-mesenchymal transition of lung epithelial cells in response to cigarette smoke. Exp Ther Med 16:12-20 (2018). Read more (PubMed: 29977354) »
See all 2 Publications for this product

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