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  1. Link

    gdf15-antibody-epr69822-ab180929.pdf

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Cell Biology Cell Cycle Cell Division Cytokinesis
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RecombinantRabMAb

Recombinant Anti-GDF15 antibody [EPR6982(2)] (ab180929)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (2)

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Western blot - Anti-GDF15 antibody [EPR6982(2)] (ab180929)
  • Western blot - Anti-GDF15 antibody [EPR6982(2)] (ab180929)
  • Anti-GDF15 antibody [EPR6982(2)] (ab180929)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6982(2)] to GDF15
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Protein
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Recombinant Mouse GDF15 protein (denatured) (ab202199)
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Anti-SMURF1 antibody [1D7] (ab57573)

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Overview

  • Product name

    Anti-GDF15 antibody [EPR6982(2)]
    See all GDF15 primary antibodies
  • Description

    Rabbit monoclonal [EPR6982(2)] to GDF15
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • LnCap and HepG2 cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6982(2)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Cytokinesis
    • Signal Transduction
    • Growth Factors/Hormones
    • TGF
    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Bone
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Hormones

Associated products

  • Alternative Versions

    • Anti-GDF15 antibody [EPR6982(2)] - BSA and Azide free (ab250263)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Hep G2 whole cell lysate (ab166833)
  • Recombinant Protein

    • Recombinant Mouse GDF15 protein (denatured) (ab202199)
  • Related Products

    • Recombinant human GDF15 protein (ab50077)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).

Target

  • Tissue specificity

    Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.
  • Sequence similarities

    Belongs to the TGF-beta family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q99988 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9518 Human
    • Omim: 605312 Human
    • SwissProt: Q99988 Human
    • Unigene: 616962 Human
    • Alternative names

      • GDF 15 antibody
      • GDF-15 antibody
      • Gdf15 antibody
      • GDF15_HUMAN antibody
      • Growth differentiation factor 15 antibody
      • Growth/differentiation factor 15 antibody
      • Macrophage inhibitory cytokine 1 antibody
      • MIC 1 antibody
      • Mic-1 antibody
      • MIC1 antibody
      • NAG 1 antibody
      • NAG-1 antibody
      • NAG1 antibody
      • NRG 1 antibody
      • NRG-1 antibody
      • NRG1 antibody
      • NSAID (nonsteroidal anti inflammatory drug) activated protein 1 antibody
      • NSAID antibody
      • NSAID regulated protein 1 antibody
      • NSAID-activated gene 1 protein antibody
      • NSAID-regulated gene 1 protein antibody
      • PDF antibody
      • PLAB antibody
      • Placental bone morphogenetic protein antibody
      • Placental bone morphogenic protein antibody
      • Placental TGF beta antibody
      • Placental TGF-beta antibody
      • Prostate differentiation factor antibody
      • PTGF beta antibody
      • PTGFB antibody
      see all

    Images

    • Western blot - Anti-GDF15 antibody [EPR6982(2)] (ab180929)
      Western blot - Anti-GDF15 antibody [EPR6982(2)] (ab180929)
      Anti-GDF15 antibody [EPR6982(2)] (ab180929) at 1/1000 dilution + HepG2 cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase Conjugate at 1/1000 dilution

      Predicted band size: 34 kDa

    • Western blot - Anti-GDF15 antibody [EPR6982(2)] (ab180929)
      Western blot - Anti-GDF15 antibody [EPR6982(2)] (ab180929)
      Anti-GDF15 antibody [EPR6982(2)] (ab180929) at 1/5000 dilution + LnCap cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 34 kDa

    • Anti-GDF15 antibody [EPR6982(2)] (ab180929)
      Anti-GDF15 antibody [EPR6982(2)] (ab180929)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (2)

    Publishing research using ab180929? Please let us know so that we can cite the reference in this datasheet.

    ab180929 has been referenced in 2 publications.

    • Tian Q  et al. Development and Validation of a Ferroptosis-Related Gene Signature for Overall Survival Prediction in Lung Adenocarcinoma. Front Cell Dev Biol 9:684259 (2021). PubMed: 34307361
    • Zhao C  et al. C5a induces A549 cell proliferation of non-small cell lung cancer via GDF15 gene activation mediated by GCN5-dependent KLF5 acetylation. Oncogene 37:4821-4837 (2018). PubMed: 29773900

    Customer reviews and Q&As

    Show All Reviews Q&A
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