Anti-GDF6 antibody (ab73288)

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Key features and details

  • Rabbit polyclonal to GDF6
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab73288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).

Target

  • Relevance

    GDF6 (Growth/differentiation factor 6) is expressed in hypertrophic chondrocytes during embryonic development of long bones and is required for normal formation of bones and joints in the limbs, skull, and axial skeleton. It plays a key role in establishing boundaries between skeletal elements during development. The functional form of GDF6 is a disulfide-linked homodimer of two 120 amino acid polypeptide chains obtained by proteolytic processing of a biologically inactive precursor protein.

  • Cellular localization

    Secreted

  • Database links

    • Alternative names

      • bmp 13 antibody
      • bmp13 antibody
      • Cartilage Derived Morphogenetic Protein 2 antibody
      • cdmp 2 antibody
      • CDMP2 antibody
      • gdf 6 antibody
      • GDF16 antibody
      • Growth differentiation factor 6 antibody
      • Growth/differentiation factor 16 antibody
      • Growth/differentiation factor 6 antibody
      • KFM antibody
      • KFS antibody
      • KFS1 antibody
      • KFSL antibody
      • Klip Feil malformation antibody
      • Klippel Feil malformation antibody
      • Klippel Feil syndrome antibody
      • LCA17 antibody
      • MCOP4 antibody
      • MCOPCB6 antibody
      • SCDO4 antibody
      • SGM1 antibody
      see all

    Images

    • Western blot - Anti-GDF6 antibody (ab73288)
      Western blot - Anti-GDF6 antibody (ab73288)
      Lane 1 : Anti-GDF6 antibody (ab73288) at 0.5 µg/ml
      Lane 2 : Anti-GDF6 antibody (ab73288) at 1 µg/ml

      All lanes : SK-N-SH lysate

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 51 kDa
      Observed band size: 55 kDa
      why is the actual band size different from the predicted?

    • Western blot - Anti-GDF6 antibody (ab73288)
      Western blot - Anti-GDF6 antibody (ab73288)
      All lanes : Anti-GDF6 antibody (ab73288) at 1 µg/ml

      Lane 1 : Recombinant Human GDF6 protein (ab50230) at 0.1 µg
      Lane 2 : Recombinant Human GDF6 protein (ab50230) at 0.01 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 51 kDa


      Exposure time: 30 seconds


      ab50230 is a homodimer consisting of two 120aa monomers. The homodimer format is expected to run at 27kDa, so the observed band is thought to be the monomer form.

    References (0)

    Publishing research using ab73288? Please let us know so that we can cite the reference in this datasheet.

    ab73288 has not yet been referenced specifically in any publications.

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