Key features and details
- Mouse monoclonal [6E4B2] to GDF8 / Myostatin
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-GDF8 / Myostatin antibody [6E4B2]
See all GDF8 / Myostatin primary antibodies
DescriptionMouse monoclonal [6E4B2] to GDF8 / Myostatin
Tested applicationsSuitable for: IHC-P, WB, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Goat, Horse, Chicken, Cow, Dog, Pig
Recombinant fragment corresponding to Human GDF8/ Myostatin aa 24-266. Recombinant fragment correspondingn to propeptide domain of human GDF8/ Myostatin expressed in E coli
NENSEQKENVEKEGLCNACTWRQNTKSSRIEAIKIQILSKLRLETAPNIS KDVIRQLLPK APPLRELIDQYDVQRDDSSDGSLEDDDYHATTETIITM PTESDFLMQVDGKPKCCFFKFS SKIQYNKVVKAQLWIYLRPVETPTTV FVQILRLIKPMKDGTRYTGIRSLKLDMNPGTGIW QSIDVKTVLQNWLK QPESNLGIEIKALDENGHDLAVTFPGPGEDGLNPFLEVKVTDTPKR SR R
Database link: O14793
- Human GDF8 / Myostatin (AA:24-266 ) recombinant protein; LNcap cell lysate; LNcap cells; Human cervical cancer tissue and Human liver cancer tissue.
This product was changed from ascites to supernatant. Lot no’s high than GR285002-6 are from Tissue Culture Supernatant
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab201954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/1000.|
|WB||1/500 - 1/2000. Predicted molecular weight: 43 kDa.|
|Flow Cyt||1/200 - 1/400.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionActs specifically as a negative regulator of skeletal muscle growth.
Sequence similaritiesBelongs to the TGF-beta family.
- Information by UniProt
- GDF 8 antibody
- GDF-8 antibody
- GDF8 antibody
Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954) at 1/500 dilution + LNcap cell lysate
Predicted band size: 43 kDa
Flow cytometric analysis of LNcap cells labeling GDF8 / Myostatin using ab201954 at 1/200 dilution (green) and negative control (red).
Immunohistochemical analysis of Human liver cancer tissue labeling GDF8 / Myostatin using ab201954 at 1/200 dilution.
Immunohistochemical analysis of Human cervical cancer tissue labeling GDF8 / Myostatin using ab201954 at 1/200 dilution.
Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954) at 1/500 dilution + Human GDF8 / Myostatin (AA:24-266 ) recombinant protein
Predicted band size: 43 kDa
Expected MW is 28.9 kDa
ab201954 has not yet been referenced specifically in any publications.