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    gdf8-myostatin-antibody-6e4b2-ab201954.pdf

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Signal Transduction Growth Factors/Hormones TGF
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Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954)

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  • Western blot - Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954)
  • Flow Cytometry - Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954)
  • Western blot - Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954)

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  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-GDF8 / Myostatin antibody [6E4B2]
    See all GDF8 / Myostatin primary antibodies
  • Description

    Mouse monoclonal [6E4B2] to GDF8 / Myostatin
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Goat, Horse, Chicken, Cow, Dog, Pig
  • Immunogen

    Recombinant fragment corresponding to Human GDF8/ Myostatin aa 24-266. Recombinant fragment correspondingn to propeptide domain of human GDF8/ Myostatin expressed in E coli
    Sequence:

    NENSEQKENVEKEGLCNACTWRQNTKSSRIEAIKIQILSKLRLETAPNIS KDVIRQLLPK APPLRELIDQYDVQRDDSSDGSLEDDDYHATTETIITM PTESDFLMQVDGKPKCCFFKFS SKIQYNKVVKAQLWIYLRPVETPTTV FVQILRLIKPMKDGTRYTGIRSLKLDMNPGTGIW QSIDVKTVLQNWLK QPESNLGIEIKALDENGHDLAVTFPGPGEDGLNPFLEVKVTDTPKR SR R


    Database link: O14793

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human GDF8 / Myostatin (AA:24-266 ) recombinant protein; LNcap cell lysate; LNcap cells; Human cervical cancer tissue and Human liver cancer tissue.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR285002-6 are from Tissue Culture Supernatant

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    6E4B2
  • Isotype

    IgG2b
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • TGF
    • Cancer
    • Growth factors
    • TGF

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • Recombinant Protein

    • Recombinant human GDF8 / Myostatin protein (ab50097)

Applications

Our Abpromise guarantee covers the use of ab201954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/1000.
WB 1/500 - 1/2000. Predicted molecular weight: 43 kDa.
Flow Cyt 1/200 - 1/400.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Acts specifically as a negative regulator of skeletal muscle growth.
  • Sequence similarities

    Belongs to the TGF-beta family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession O14793 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2660 Human
    • Entrez Gene: 17700 Mouse
    • Entrez Gene: 29152 Rat
    • Omim: 601788 Human
    • SwissProt: O14793 Human
    • SwissProt: O08689 Mouse
    • SwissProt: O35312 Rat
    • Unigene: 41565 Human
    • Unigene: 3514 Mouse
    • Unigene: 44460 Rat
    see all
  • Alternative names

    • GDF 8 antibody
    • GDF-8 antibody
    • GDF8 antibody
    • GDF8_HUMAN antibody
    • Growth differentiation factor 8 antibody
    • Growth/differentiation factor 8 antibody
    • MSLHP antibody
    • MSTN antibody
    • Myostatin antibody
    • OTTHUMP00000163498 antibody
    see all

Images

  • Western blot - Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954)
    Western blot - Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954)
    Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954) at 1/500 dilution + LNcap cell lysate

    Predicted band size: 43 kDa

  • Flow Cytometry - Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954)
    Flow Cytometry - Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954)

    Flow cytometric analysis of LNcap cells labeling GDF8 / Myostatin using ab201954 at 1/200 dilution (green) and negative control (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954)

    Immunohistochemical analysis of Human liver cancer tissue labeling GDF8 / Myostatin using ab201954 at 1/200 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954)

    Immunohistochemical analysis of Human cervical cancer tissue labeling GDF8 / Myostatin using ab201954 at 1/200 dilution.

  • Western blot - Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954)
    Western blot - Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954)
    Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954) at 1/500 dilution + Human GDF8 / Myostatin (AA:24-266 ) recombinant protein

    Predicted band size: 43 kDa



    Expected MW is 28.9 kDa

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Datasheets and documents

    • Datasheet
  • References

    ab201954 has not yet been referenced specifically in any publications.

    Publishing research using ab201954? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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