Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954)
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Overview
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Product name
Anti-GDF8 / Myostatin antibody [6E4B2]
See all GDF8 / Myostatin primary antibodies -
Description
Mouse monoclonal [6E4B2] to GDF8 / Myostatin -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Goat, Horse, Chicken, Cow, Dog, Pig -
Immunogen
Recombinant fragment corresponding to Human GDF8/ Myostatin aa 24-266. Recombinant fragment correspondingn to propeptide domain of human GDF8/ Myostatin expressed in E coli
Sequence:NENSEQKENVEKEGLCNACTWRQNTKSSRIEAIKIQILSKLRLETAPNIS KDVIRQLLPK APPLRELIDQYDVQRDDSSDGSLEDDDYHATTETIITM PTESDFLMQVDGKPKCCFFKFS SKIQYNKVVKAQLWIYLRPVETPTTV FVQILRLIKPMKDGTRYTGIRSLKLDMNPGTGIW QSIDVKTVLQNWLK QPESNLGIEIKALDENGHDLAVTFPGPGEDGLNPFLEVKVTDTPKR SR R
Database link: O14793 -
Positive control
- Human GDF8 / Myostatin (AA:24-266 ) recombinant protein; LNcap cell lysate; LNcap cells; Human cervical cancer tissue and Human liver cancer tissue.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR285002-6 are from Tissue Culture Supernatant
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
6E4B2 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab201954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | 1/200 - 1/1000. | |
WB | 1/500 - 1/2000. Predicted molecular weight: 43 kDa. | |
Flow Cyt | 1/200 - 1/400. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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Target
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Function
Acts specifically as a negative regulator of skeletal muscle growth. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2660 Human
- Entrez Gene: 17700 Mouse
- Entrez Gene: 29152 Rat
- Omim: 601788 Human
- SwissProt: O14793 Human
- SwissProt: O08689 Mouse
- SwissProt: O35312 Rat
- Unigene: 41565 Human
see all -
Alternative names
- GDF 8 antibody
- GDF-8 antibody
- GDF8 antibody
see all
Images
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Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954) at 1/500 dilution + LNcap cell lysate
Predicted band size: 43 kDa -
Flow cytometric analysis of LNcap cells labeling GDF8 / Myostatin using ab201954 at 1/200 dilution (green) and negative control (red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954)
Immunohistochemical analysis of Human liver cancer tissue labeling GDF8 / Myostatin using ab201954 at 1/200 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954)
Immunohistochemical analysis of Human cervical cancer tissue labeling GDF8 / Myostatin using ab201954 at 1/200 dilution.
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Anti-GDF8 / Myostatin antibody [6E4B2] (ab201954) at 1/500 dilution + Human GDF8 / Myostatin (AA:24-266 ) recombinant protein
Predicted band size: 43 kDaExpected MW is 28.9 kDa
Datasheets and documents
References
ab201954 has not yet been referenced specifically in any publications.