Key features and details
- Rabbit polyclonal to GDI1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Product nameAnti-GDI1 antibody
See all GDI1 primary antibodies
DescriptionRabbit polyclonal to GDI1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human, Pig
Predicted to work with: Rat, Cow, Dog, Chimpanzee, Macaque monkey, Orangutan
Recombinant fragment (His-T7-tag) corresponding to Human GDI1 aa 1-274. (Expressed in E.coli).
MDEEYDVIVLGTGLTECILSGIMSVNGKKVLHMDRNPYYGGESSSITPLE ELYKRFQLLEGPPESMGRGRDWNVDLIPKFLMANGQLVKMLLYTEVTRYL DFKVVEGSFVYKGGKIYKVPSTETEALASNLMGMFEKRRFRKFLVFVANF DENDPKTFEGVDPQTTSMRDVYRKFDLGQDVIDFTGHALALYRTDDYLDQ PCLETVNRIKLYSESLARYGKSPYLYPLYGLGELPQGFARLSAIYGGTYM LNKPVDDIIMENGKVVGVKSEGEV
Database link: P31150
- WB: Pig and mouse brain lysate; U-87MG cell lysate; Recombinant human GDI1 protein. IHC-P: Human liver, breast cancer and stomach tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 51 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionRegulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
Tissue specificityBrain; predominant in neural and sensory tissues.
Involvement in diseaseDefects in GDI1 are the cause of mental retardation X-linked type 41 (MRX41) [MIM:300104]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
Defects in GDI1 are the cause of mental retardation X-linked type 48 (MRX48) [MIM:300104]; also known as MRX3.
Sequence similaritiesBelongs to the Rab GDI family.
- Information by UniProt
- 1A antibody
- FLJ41411 antibody
- GDI-1 antibody
Formalin-fixed, paraffin-embedded human stomach tissue stained for GDI1 using ab233149 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for GDI1 using ab233149 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human liver tissue stained for GDI1 using ab233149 at 10 µg/ml in immunohistochemical analysis. DAB staining.
All lanes : Anti-GDI1 antibody (ab233149) at 2 µg/ml
Lane 1 : Pig brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : U-87MG (human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 51 kDa
Anti-GDI1 antibody (ab233149) at 2 µg/ml + Recombinant human GDI1 protein
Predicted band size: 51 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233149 has not yet been referenced specifically in any publications.