Overview

  • Product name

    Anti-GDI1 antibody [EPR13195]
    See all GDI1 primary antibodies
  • Description

    Rabbit monoclonal [EPR13195] to GDI1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GDI1 aa 1-100. The exact sequence is proprietary.
    Database link: P31150

  • Positive control

    • U87 MG, HeLa, SH-SY5Y and Human fetal brain lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13195
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30 - 1/50.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).

Target

  • Function

    Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
  • Tissue specificity

    Brain; predominant in neural and sensory tissues.
  • Involvement in disease

    Defects in GDI1 are the cause of mental retardation X-linked type 41 (MRX41) [MIM:300104]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
    Defects in GDI1 are the cause of mental retardation X-linked type 48 (MRX48) [MIM:300104]; also known as MRX3.
  • Sequence similarities

    Belongs to the Rab GDI family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1A antibody
    • FLJ41411 antibody
    • GDI-1 antibody
    • gdi1 antibody
    • GDIA_HUMAN antibody
    • GDIL antibody
    • GDP dissociation inhibitor 1 antibody
    • Guanosine diphosphate dissociation inhibitor 1 antibody
    • Mental retardation X-linked 41 antibody
    • Mental retardation X-linked 48 antibody
    • MRX41 antibody
    • MRX48 antibody
    • Oligophrenin 2 antibody
    • Oligophrenin-2 antibody
    • OPHN2 antibody
    • Protein XAP 4 antibody
    • Protein XAP-4 antibody
    • Protein XAP4 antibody
    • Rab GDI alpha antibody
    • Rab GDP dissociation inhibitor alpha antibody
    • RABGD1A antibody
    • RABGDIA antibody
    • XAP 4 antibody
    see all

Images

  • All lanes : Anti-GDI1 antibody [EPR13195] (ab181213) at 1/10000 dilution

    Lane 1 : U87 MG cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : SH-SY5Y cell lysate
    Lane 4 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa

  • Flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling GDI1 with ab181213 at 1/40 dilution (red), or a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as a secondary antibody.

  • Western blot analysis of immunoprecipitation pellet from Human fetal brain lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181213 at 1/50 dilution.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

     

     

References

ab181213 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181213.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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