Product nameAnti-GDI1 antibody [EPR13196(B)]
See all GDI1 primary antibodies
DescriptionRabbit monoclonal [EPR13196(B)] to GDI1
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human GDI1 aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P31150
- U87-MG, HeLa, SH-SY5Y and Human fetal brain lysates; Permeabilized SH-SY5Y cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab175194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 51 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionRegulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
Tissue specificityBrain; predominant in neural and sensory tissues.
Involvement in diseaseDefects in GDI1 are the cause of mental retardation X-linked type 41 (MRX41) [MIM:300104]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
Defects in GDI1 are the cause of mental retardation X-linked type 48 (MRX48) [MIM:300104]; also known as MRX3.
Sequence similaritiesBelongs to the Rab GDI family.
- Information by UniProt
- 1A antibody
- FLJ41411 antibody
- GDI-1 antibody
All lanes : Anti-GDI1 antibody [EPR13196(B)] (ab175194) at 1/10000 dilution
Lane 1 : U87-MG lysates
Lane 2 : HeLa lysates
Lane 3 : SH-SY5Y lysates
Lane 4 : Human fetal brain lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 51 kDa
Flow cytometric analysis of permeabilized SH-SY5Y cells labeling GDI1 with ab175194 at 1/10 dilution (red) or a Rabbit IgG (negative) (green).
ab175194 has not yet been referenced specifically in any publications.