Recombinant Anti-GDI1 antibody [EPR13196(B)] (ab175194)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-GDI1 antibody [EPR13196(B)]
See all GDI1 primary antibodies -
Description
Rabbit monoclonal [EPR13196(B)] to GDI1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human GDI1 aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P31150 -
Positive control
- U87-MG, HeLa, SH-SY5Y and Human fetal brain lysates; Permeabilized SH-SY5Y cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13196(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab175194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 51 kDa. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. -
Tissue specificity
Brain; predominant in neural and sensory tissues. -
Involvement in disease
Defects in GDI1 are the cause of mental retardation X-linked type 41 (MRX41) [MIM:300104]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
Defects in GDI1 are the cause of mental retardation X-linked type 48 (MRX48) [MIM:300104]; also known as MRX3. -
Sequence similarities
Belongs to the Rab GDI family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 2664 Human
- Entrez Gene: 14567 Mouse
- Entrez Gene: 25183 Rat
- Omim: 300104 Human
- SwissProt: P31150 Human
- SwissProt: P50396 Mouse
- SwissProt: P50398 Rat
- Unigene: 74576 Human
see all -
Alternative names
- 1A antibody
- FLJ41411 antibody
- GDI-1 antibody
see all
Images
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![Western blot - Anti-GDI1 antibody [EPR13196(B)] (ab175194)](https://www.abcam.com/ps/products/175/ab175194/Images/ab175194-201533-ab1751941.jpg)
Western blot - Anti-GDI1 antibody [EPR13196(B)] (ab175194)All lanes : Anti-GDI1 antibody [EPR13196(B)] (ab175194) at 1/10000 dilution
Lane 1 : U87-MG lysates
Lane 2 : HeLa lysates
Lane 3 : SH-SY5Y lysates
Lane 4 : Human fetal brain lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 51 kDa -
![Flow Cytometry - Anti-GDI1 antibody [EPR13196(B)] (ab175194)](https://www.abcam.com/ps/products/175/ab175194/Images/ab175194-201532-ab1751942.jpg)
Flow Cytometry - Anti-GDI1 antibody [EPR13196(B)] (ab175194)Flow cytometric analysis of permeabilized SH-SY5Y cells labeling GDI1 with ab175194 at 1/10 dilution (red) or a Rabbit IgG (negative) (green).
Datasheets and documents
References
ab175194 has not yet been referenced specifically in any publications.
