Recombinant Anti-GDI1 antibody [EPR13196(B)] (ab175194)
![Western blot - Anti-GDI1 antibody [EPR13196(B)] (ab175194)](https://www.abcam.com/ps/products/175/ab175194/Images/ab175194-201533-ab1751941.jpg "Western blot - Anti-GDI1 antibody [EPR13196(B)] (ab175194)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13196(B)] to GDI1
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
-
Product name
Anti-GDI1 antibody [EPR13196(B)]
See all GDI1 primary antibodies -
Description
Rabbit monoclonal [EPR13196(B)] to GDI1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
-
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human GDI1 aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P31150 -
Positive control
- U87-MG, HeLa, SH-SY5Y and Human fetal brain lysates; Permeabilized SH-SY5Y cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13196(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| ICC |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 51 kDa.
|
|
| Flow Cyt |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 51 kDa. |
|
Flow Cyt
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. -
Tissue specificity
Brain; predominant in neural and sensory tissues. -
Involvement in disease
Defects in GDI1 are the cause of mental retardation X-linked type 41 (MRX41) [MIM:300104]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
Defects in GDI1 are the cause of mental retardation X-linked type 48 (MRX48) [MIM:300104]; also known as MRX3. -
Sequence similarities
Belongs to the Rab GDI family. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 2664 Human
- Entrez Gene: 14567 Mouse
- Entrez Gene: 25183 Rat
- Omim: 300104 Human
- SwissProt: P31150 Human
- SwissProt: P50396 Mouse
- SwissProt: P50398 Rat
- Unigene: 74576 Human
see all -
Alternative names
- 1A antibody
- FLJ41411 antibody
- GDI-1 antibody
see all
Images
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Western blot - Anti-GDI1 antibody [EPR13196(B)] (ab175194)All lanes : Anti-GDI1 antibody [EPR13196(B)] (ab175194) at 1/10000 dilution
Lane 1 : U87-MG lysates
Lane 2 : HeLa lysates
Lane 3 : SH-SY5Y lysates
Lane 4 : Human fetal brain lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 51 kDa -
![Flow Cytometry - Anti-GDI1 antibody [EPR13196(B)] (ab175194)](https://www.abcam.com/ps/products/175/ab175194/Images/ab175194-201532-ab1751942.jpg)
Flow Cytometry - Anti-GDI1 antibody [EPR13196(B)] (ab175194)Flow cytometric analysis of permeabilized SH-SY5Y cells labeling GDI1 with ab175194 at 1/10 dilution (red) or a Rabbit IgG (negative) (green).
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![Anti-GDI1 antibody [EPR13196(B)] (ab175194)](https://www.abcam.com/ps/products/175/ab175194/Images/ab175194-3-benefits-of-recombinant-antibodies.png)
Anti-GDI1 antibody [EPR13196(B)] (ab175194)
Datasheets and documents
References (0)
ab175194 has not yet been referenced specifically in any publications.
