Recombinant
RabMAb

Anti-GDI1 antibody [EPR13196(B)] (ab175194)

Overview

  • Product name
    Anti-GDI1 antibody [EPR13196(B)]
    See all GDI1 primary antibodies
  • Description
    Rabbit monoclonal [EPR13196(B)] to GDI1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human GDI1 aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P31150

  • Positive control
    • U87-MG, HeLa, SH-SY5Y and Human fetal brain lysates; Permeabilized SH-SY5Y cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR13196(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 51 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
    • Tissue specificity
      Brain; predominant in neural and sensory tissues.
    • Involvement in disease
      Defects in GDI1 are the cause of mental retardation X-linked type 41 (MRX41) [MIM:300104]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
      Defects in GDI1 are the cause of mental retardation X-linked type 48 (MRX48) [MIM:300104]; also known as MRX3.
    • Sequence similarities
      Belongs to the Rab GDI family.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1A antibody
      • FLJ41411 antibody
      • GDI-1 antibody
      • gdi1 antibody
      • GDIA_HUMAN antibody
      • GDIL antibody
      • GDP dissociation inhibitor 1 antibody
      • Guanosine diphosphate dissociation inhibitor 1 antibody
      • Mental retardation X-linked 41 antibody
      • Mental retardation X-linked 48 antibody
      • MRX41 antibody
      • MRX48 antibody
      • Oligophrenin 2 antibody
      • Oligophrenin-2 antibody
      • OPHN2 antibody
      • Protein XAP 4 antibody
      • Protein XAP-4 antibody
      • Protein XAP4 antibody
      • Rab GDI alpha antibody
      • Rab GDP dissociation inhibitor alpha antibody
      • RABGD1A antibody
      • RABGDIA antibody
      • XAP 4 antibody
      see all

    Images

    • All lanes : Anti-GDI1 antibody [EPR13196(B)] (ab175194) at 1/10000 dilution

      Lane 1 : U87-MG lysates
      Lane 2 : HeLa lysates
      Lane 3 : SH-SY5Y lysates
      Lane 4 : Human fetal brain lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 51 kDa

    • Flow cytometric analysis of permeabilized SH-SY5Y cells labeling GDI1 with ab175194 at 1/10 dilution (red) or a Rabbit IgG (negative) (green).

    References

    ab175194 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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