For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    gdi2-antibody-ab234986.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Protein Trafficking Vesicle Transport Regulation
Share by email

Anti-GDI2 antibody (ab234986)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-GDI2 antibody (ab234986)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDI2 antibody (ab234986)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDI2 antibody (ab234986)

Key features and details

  • Rabbit polyclonal to GDI2
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Stain
Product image
StayBlue/AP Plus (ab176915)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Knockout
Product image
Human GDI2 knockout HEK-293T cell line (ab266289)

View more associated products

Overview

  • Product name

    Anti-GDI2 antibody
    See all GDI2 primary antibodies
  • Description

    Rabbit polyclonal to GDI2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Pig, Orangutan
  • Immunogen

    Recombinant full length protein corresponding to Human GDI2 aa 1 to the C-terminus.
    Database link: P50395

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Mouse brain lysate; HepG2 and MCF7 whole cell lysates. IHC-P: Human gastric cancer and breast cancer tissues.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Hep G2 whole cell lysate (ab166833)
    • MCF7 whole cell lysate (ab29537)
    • Mouse brain tissue lysate - total protein (ab30151)
    • Mouse brain tissue lysate - total protein (ab4022)
  • Recombinant Protein

    • Recombinant Human GDI2 protein (ab180348)
  • Related Products

    • Recombinant Human GDI2 protein (ab180348)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab234986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/500 - 1/5000. Predicted molecular weight: 51 kDa.
IHC-P
1/20 - 1/200.
Notes
WB
1/500 - 1/5000. Predicted molecular weight: 51 kDa.
IHC-P
1/20 - 1/200.

Target

  • Function

    Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the Rab GDI family.
  • Cellular localization

    Cytoplasm. Membrane.
  • Target information above from: UniProt accession P50395 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 509832 Cow
    • Entrez Gene: 403818 Dog
    • Entrez Gene: 2665 Human
    • Entrez Gene: 14569 Mouse
    • Entrez Gene: 100172200 Orangutan
    • Entrez Gene: 414427 Pig
    • Entrez Gene: 29662 Rat
    • Omim: 600767 Human
    • SwissProt: P50397 Cow
    • SwissProt: O97556 Dog
    • SwissProt: P50395 Human
    • SwissProt: Q61598 Mouse
    • SwissProt: Q5RCE1 Orangutan
    • SwissProt: Q6Q7J2 Pig
    • SwissProt: P50399 Rat
    • Unigene: 299055 Human
    • Unigene: 153226 Mouse
    • Unigene: 221609 Mouse
    • Unigene: 1441 Rat
    see all
  • Alternative names

    • GDI-2 antibody
    • GDI2 antibody
    • GDIB_HUMAN antibody
    • GDP dissociation inhibitor 2 antibody
    • Guanosine diphosphate dissociation inhibitor 2 antibody
    • Rab GDI beta antibody
    • Rab GDP dissociation inhibitor beta antibody
    • RABGDIB antibody
    see all

Images

  • Western blot - Anti-GDI2 antibody (ab234986)
    Western blot - Anti-GDI2 antibody (ab234986)
    All lanes : Anti-GDI2 antibody (ab234986) at 1/500 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit at 1/10000 dilution

    Predicted band size: 51 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDI2 antibody (ab234986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDI2 antibody (ab234986)

    Paraffin-embedded human gastric cancer tissue stained for GDI2 using ab234986 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDI2 antibody (ab234986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDI2 antibody (ab234986)

    Paraffin-embedded human breast cancer tissue stained for GDI2 using ab234986 at 1/100 dilution in immunohistochemical analysis.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab234986? Please let us know so that we can cite the reference in this datasheet.

ab234986 has been referenced in 1 publication.

  • Zou J  et al. MicroRNA-15b-5p exerts its tumor repressive role via targeting GDI2: A novel insight into the pathogenesis of thyroid carcinoma. Mol Med Rep 22:2723-2732 (2020). PubMed: 32945458

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Western blot abreview for Anti-GDI2 antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (liver)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-tris)
Loading amount
10 µg
Specification
liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Read More

Abcam user community

Verified customer

Submitted Sep 30 2021

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.