Anti-GDI2 antibody (ab234986)
Key features and details
- Rabbit polyclonal to GDI2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-GDI2 antibody
See all GDI2 primary antibodies -
Description
Rabbit polyclonal to GDI2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Pig, Orangutan
-
Immunogen
Recombinant full length protein corresponding to Human GDI2 aa 1 to the C-terminus.
Database link: P50395 -
Positive control
- WB: Mouse brain lysate; HepG2 and MCF7 whole cell lysates. IHC-P: Human gastric cancer and breast cancer tissues.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/500 - 1/5000. Predicted molecular weight: 51 kDa.
|
| IHC-P |
1/20 - 1/200.
|
| Notes |
|---|
|
WB
1/500 - 1/5000. Predicted molecular weight: 51 kDa. |
|
IHC-P
1/20 - 1/200. |
Target
-
Function
Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the Rab GDI family. -
Cellular localization
Cytoplasm. Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 509832 Cow
- Entrez Gene: 403818 Dog
- Entrez Gene: 2665 Human
- Entrez Gene: 14569 Mouse
- Entrez Gene: 100172200 Orangutan
- Entrez Gene: 414427 Pig
- Entrez Gene: 29662 Rat
- Omim: 600767 Human
see all -
Alternative names
- GDI-2 antibody
- GDI2 antibody
- GDIB_HUMAN antibody
see all
Images
-
Western blot - Anti-GDI2 antibody (ab234986)All lanes : Anti-GDI2 antibody (ab234986) at 1/500 dilution
Lane 1 : Mouse brain lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 51 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDI2 antibody (ab234986)Paraffin-embedded human gastric cancer tissue stained for GDI2 using ab234986 at 1/100 dilution in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDI2 antibody (ab234986)Paraffin-embedded human breast cancer tissue stained for GDI2 using ab234986 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab234986 has been referenced in 1 publication.
- Zou J et al. MicroRNA-15b-5p exerts its tumor repressive role via targeting GDI2: A novel insight into the pathogenesis of thyroid carcinoma. Mol Med Rep 22:2723-2732 (2020). PubMed: 32945458