Anti-GDI2 antibody (ab234986)
- Datasheet
- References
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Overview
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Product name
Anti-GDI2 antibody
See all GDI2 primary antibodies -
Description
Rabbit polyclonal to GDI2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Pig, Orangutan
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Immunogen
Recombinant full length protein corresponding to Human GDI2 aa 1-445.
Sequence:MNEEYDVIVLGTGLTECILSGIMSVNGKKVLHMDRNPYYGGESASITPLE DLYKRFKIPGSPPESMGRGRDWNVDLIPKFLMANGQLVKMLLYTEVTRYL DFKVTEGSFVYKGGKIYKVPSTEAEALASSLMGLFEKRRFRKFLVYVANF DEKDPRTFEGIDPKKTTMRDVYKKFDLGQDVIDFTGHALALYRTDDYLDQ PCYETINRIKLYSESLARYGKSPYLYPLYGLGELPQGFARLSAIYGGTYM LNKPIEEIIVQNGKVIGVKSEGEIARCKQLICDPSYVKDRVEKVGQVIRV ICILSHPIKNTNDANSCQIIIPQNQVNRKSDIYVCMISFAHNVAAQGKYI AIVSTTVETKEPEKEIRPALELLEPIEQKFVSISDLLVPKDLGTESQIFI SRTYDATTHFETTCDDIKNIYKRMTGSEFDFEEMKRKKNDIYGED
Database link: P50395 -
Positive control
- WB: Mouse brain lysate; HepG2 and MCF7 whole cell lysates. IHC-P: Human gastric cancer and breast cancer tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab234986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/5000. Predicted molecular weight: 51 kDa. | |
| IHC-P | 1/20 - 1/200. |
Target
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Function
Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the Rab GDI family. -
Cellular localization
Cytoplasm. Membrane. - Information by UniProt
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Database links
- Entrez Gene: 509832 Cow
- Entrez Gene: 403818 Dog
- Entrez Gene: 2665 Human
- Entrez Gene: 14569 Mouse
- Entrez Gene: 100172200 Orangutan
- Entrez Gene: 414427 Pig
- Entrez Gene: 29662 Rat
- Omim: 600767 Human
see all -
Alternative names
- GDI-2 antibody
- GDI2 antibody
- GDIB_HUMAN antibody
see all
Images
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Western blot - Anti-GDI2 antibody (ab234986)All lanes : Anti-GDI2 antibody (ab234986) at 1/500 dilution
Lane 1 : Mouse brain lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 51 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDI2 antibody (ab234986)Paraffin-embedded human gastric cancer tissue stained for GDI2 using ab234986 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDI2 antibody (ab234986)Paraffin-embedded human breast cancer tissue stained for GDI2 using ab234986 at 1/100 dilution in immunohistochemical analysis.
References
ab234986 has not yet been referenced specifically in any publications.
