Key features and details
- Rabbit polyclonal to GDNF
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-GDNF antibody
See all GDNF primary antibodies
DescriptionRabbit polyclonal to GDNF
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
Synthetic peptide corresponding to an internal sequence of Human GDNF.
- Rat brain and human lung tissue; Recombinant Human GDNF protein.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab119473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 24 kDa.
The detection limit for GDNF is approximately 2.5ng/lane under non-reducing and reducing conditions. GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionNeurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
Tissue specificityIn the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen.
Involvement in diseaseDefects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia.
Sequence similaritiesBelongs to the TGF-beta family. GDNF subfamily.
- Information by UniProt
- Astrocyte derived trophic factor antibody
- Astrocyte derived trophic factor 1 antibody
- Astrocyte-derived trophic factor antibody
ab119473 at 1µg/ml staining GDNF in by Paraffin-embedded Human lung tissue by Immunohistochemistry.
ab119473 at 1µg/ml staining GDNF in by Paraffin-embedded Rat brain tissue by Immunohistochemistry.
All lanes : Anti-GDNF antibody (ab119473) at 1 µg/ml
Lane 1 : Recombinant Human GDNF protein at 0.01 µg
Lane 2 : Recombinant Human GDNF protein at 0.005 µg
Lane 3 : Recombinant Human GDNF protein at 0.0025 µg
Predicted band size: 24 kDa
ab119473 has been referenced in 3 publications.
- Meftahi GH et al. Chronic administration of methylphenidate did not affect memory and GDNF levels but increase astrogliosis in adult male rat's hippocampus. J Chem Neuroanat 108:101818 (2020). PubMed: 32485223
- Li L et al. Ascorbic Acid Facilitates Neural Regeneration After Sciatic Nerve Crush Injury. Front Cell Neurosci 13:108 (2019). PubMed: 30949031
- Marton S et al. Ibogaine Administration Modifies GDNF and BDNF Expression in Brain Regions Involved in Mesocorticolimbic and Nigral Dopaminergic Circuits. Front Pharmacol 10:193 (2019). PubMed: 30890941