Overview

  • Product name

  • Description

    Rabbit polyclonal to GDNF
  • Host species

    Rabbit
  • Specificity

    GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to an internal sequence of Human GDNF.

  • Positive control

    • Rat brain tissue; Recombinant Human GDNF protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab119473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa.

The detection limit for GDNF is approximately 2.5ng/lane under non-reducing and reducing conditions. GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot.

IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
  • Tissue specificity

    In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen.
  • Involvement in disease

    Defects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
    Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia.
  • Sequence similarities

    Belongs to the TGF-beta family. GDNF subfamily.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Astrocyte derived trophic factor antibody
    • Astrocyte derived trophic factor 1 antibody
    • Astrocyte-derived trophic factor antibody
    • ATF 1 antibody
    • ATF 2 antibody
    • Atf antibody
    • ATF1 antibody
    • ATF2 antibody
    • gdnf antibody
    • GDNF_HUMAN antibody
    • Glial cell derived neurotrophic factor antibody
    • Glial Cell Line Derived Neurotrophic Factor antibody
    • Glial cell line-derived neurotrophic factor antibody
    • Glial derived neurotrophic factor antibody
    • HFB1 GDNF antibody
    • hGDNF antibody
    • HSCR3 antibody
    see all

Images

  • ab119473 at 1µg/ml staining GDNF in by Paraffin-embedded Rat brain tissue by Immunohistochemistry.
  • All lanes : Anti-GDNF antibody (ab119473) at 1 µg/ml

    Lane 1 : Recombinant Human GDNF protein at 0.01 µg
    Lane 2 : Recombinant Human GDNF protein at 0.005 µg
    Lane 3 : Recombinant Human GDNF protein at 0.0025 µg

    Predicted band size: 24 kDa

References

This product has been referenced in:

  • Li L  et al. Ascorbic Acid Facilitates Neural Regeneration After Sciatic Nerve Crush Injury. Front Cell Neurosci 13:108 (2019). Read more (PubMed: 30949031) »
  • Marton S  et al. Ibogaine Administration Modifies GDNF and BDNF Expression in Brain Regions Involved in Mesocorticolimbic and Nigral Dopaminergic Circuits. Front Pharmacol 10:193 (2019). Read more (PubMed: 30890941) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

I would like to reassure you that ab119473 is tested and covered by our 6 month guarantee for use inWB and rat samples. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.

Reviewing this case, I would like to offer some suggestions to help optimize the results. I would also appreciate if you can confirm some further details:

1. Please confirm what expression level are you expecting from these samples and at this developmental stage (you mention rat pups, what age are they?). I am sorry I am not able to find much informationto confirmexpression level of this protein in juvenile rat brain. Do you have any details?

Was any positive control included, for example brain from adult rat?

2. Try a higher concentration of antibody, 1:500

3. Has the transfer to the membrane and quality of the sample been assessedusing a loading control

4. Try BSA rather than milk to block. Changing blocking agent can sometimes help improve results.

I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

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