Anti-GDNF antibody (ab18956)
Key features and details
- Rabbit polyclonal to GDNF
- Suitable for: IHC-P, IHC-Fr, IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GDNF antibody
See all GDNF primary antibodies -
Description
Rabbit polyclonal to GDNF -
Host species
Rabbit -
Specificity
GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot. -
Tested applications
Suitable for: IHC-P, IHC-Fr, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide surrounding amino acid 195 of mouse GDNF.(Peptide available as ab51926.)
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General notes
This product is manufactured by BioVision, an Abcam company and was previously called 5098 GDNF Antibody. 5098-100 is the same size as the 100 µg size of ab18956.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.03% Proclin 300
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.015% EDTA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (5) |
Use at an assay dependent concentration.
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IHC-Fr | (1) |
Use at an assay dependent concentration.
|
IP |
Use at an assay dependent concentration.
|
|
WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
Western blot may give multiple bands. GDNF homodimerizes and has 5 isoforms along with a signal and propeptide. |
Notes |
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IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa. Western blot may give multiple bands. GDNF homodimerizes and has 5 isoforms along with a signal and propeptide. |
Target
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Function
Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. -
Tissue specificity
In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. -
Involvement in disease
Defects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. -
Sequence similarities
Belongs to the TGF-beta family. GDNF subfamily. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2668 Human
- Entrez Gene: 14573 Mouse
- Omim: 600837 Human
- SwissProt: P39905 Human
- SwissProt: P48540 Mouse
- Unigene: 248114 Human
- Unigene: 4679 Mouse
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Alternative names
- Astrocyte derived trophic factor antibody
- Astrocyte derived trophic factor 1 antibody
- Astrocyte-derived trophic factor antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (56)
ab18956 has been referenced in 56 publications.
- Liu YP et al. Exosome-Mediated miR-21 Was Involved in the Promotion of Structural and Functional Recovery Effect Produced by Electroacupuncture in Sciatic Nerve Injury. Oxid Med Cell Longev 2022:7530102 (2022). PubMed: 35132352
- George SD et al. Caloric Vestibular Stimulation Induced Enhancement of Behavior and Neurotrophic Factors in Chronic Mild Stress Induced Rats. Front Pharmacol 13:834292 (2022). PubMed: 35600855
- Zhang Q et al. Implantation of a nerve protector embedded with human GMSC-derived Schwann-like cells accelerates regeneration of crush-injured rat sciatic nerves. Stem Cell Res Ther 13:263 (2022). PubMed: 35725660
- Shadfar S et al. Methanolic Extract of Boswellia serrata Gum Protects the Nigral Dopaminergic Neurons from Rotenone-Induced Neurotoxicity. Mol Neurobiol 59:5874-5890 (2022). PubMed: 35804280
- Ucar B et al. Biomaterial based strategies to reconstruct the nigrostriatal pathway in organotypic slice co-cultures. Acta Biomater 121:250-262 (2021). PubMed: 33242639