Key features and details
- Rabbit polyclonal to GDNF
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GDNF antibody
See all GDNF primary antibodies
DescriptionRabbit polyclonal to GDNF
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human GDNF (internal sequence).
Database link: P39905
- WB: HeLa cell lysate. ICC/IF: SK-N-BE cells.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 24 kDa.|
FunctionNeurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
Tissue specificityIn the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen.
Involvement in diseaseDefects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia.
Sequence similaritiesBelongs to the TGF-beta family. GDNF subfamily.
- Information by UniProt
- Astrocyte derived trophic factor antibody
- Astrocyte derived trophic factor 1 antibody
- Astrocyte-derived trophic factor antibody
4% formaldehyde-fixed SK-N-BE cells stained for GDNF (green) using ab223347 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit ATTO 488 at 1/200 for 60 minutes at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.
(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223347 (D) Merge.
Anti-GDNF antibody (ab223347) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg
Goat Anti-Rabbit IgG: HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 24 kDa
Exposure time: 6 minutes
Blocking: 5% skimmed milk in 1X TBST.
ab223347 has not yet been referenced specifically in any publications.