Overview

  • Product name

  • Description

    Rabbit polyclonal to GDNF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human GDNF (internal sequence).
    Database link: P39905

  • Positive control

    • WB: HeLa cell lysate. ICC/IF: SK-N-BE cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab223347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000. Predicted molecular weight: 24 kDa.

Target

  • Function

    Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
  • Tissue specificity

    In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen.
  • Involvement in disease

    Defects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
    Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia.
  • Sequence similarities

    Belongs to the TGF-beta family. GDNF subfamily.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Astrocyte derived trophic factor antibody
    • Astrocyte derived trophic factor 1 antibody
    • Astrocyte-derived trophic factor antibody
    • ATF 1 antibody
    • ATF 2 antibody
    • Atf antibody
    • ATF1 antibody
    • ATF2 antibody
    • gdnf antibody
    • GDNF_HUMAN antibody
    • Glial cell derived neurotrophic factor antibody
    • Glial Cell Line Derived Neurotrophic Factor antibody
    • Glial cell line-derived neurotrophic factor antibody
    • Glial derived neurotrophic factor antibody
    • HFB1 GDNF antibody
    • hGDNF antibody
    • HSCR3 antibody
    see all

Images

  • 4% formaldehyde-fixed SK-N-BE cells stained for GDNF (green) using ab223347 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit ATTO 488 at 1/200 for 60 minutes at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.

    (A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223347 (D) Merge.

  • Anti-GDNF antibody (ab223347) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG: HRP at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 24 kDa


    Exposure time: 6 minutes


     Blocking: 5% skimmed milk in 1X TBST.

References

ab223347 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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