Key features and details
- Sheep polyclonal to GDNF
- Suitable for: Dot blot, WB, IHC (PFA fixed)
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GDNF antibody
See all GDNF primary antibodies
DescriptionSheep polyclonal to GDNF
SpecificityCross reactivity: This antibody is known to react with human, mouse, rat and other rodents. Cross reactivity with other species has not been tested. No cross reactivity with NTN. GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot.
Tested applicationsSuitable for: Dot blot, WB, IHC (PFA fixed)more details
Species reactivityReacts with: Human
Recombinant human Glial Derived Neurotrophic Factor.
General notesThis antibody has been used successfully for immunohistochemistry, immunoblotting and quantitative 2- site ELISA.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab6206 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC (PFA fixed)|
Immunohistochemistry (PFA fixed): Use at an assay dependent dilution on 4% paraformaldehyde perfusion fixed tissue cut on cryostat. See protocols given in the book "Neurotrophin Protocols" edited by RA Rush and published by Humana Press. This book is volume 169 in the Methods in Molecular Biology series.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionNeurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
Tissue specificityIn the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen.
Involvement in diseaseDefects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia.
Sequence similaritiesBelongs to the TGF-beta family. GDNF subfamily.
- Information by UniProt
- Astrocyte derived trophic factor antibody
- Astrocyte derived trophic factor 1 antibody
- Astrocyte-derived trophic factor antibody
ab6206 has not yet been referenced specifically in any publications.