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RecombinantRabMAb

Recombinant Anti-GDNF antibody [EPR2714(N)] (ab176564)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (9)

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Western blot - Anti-GDNF antibody [EPR2714(N)] (ab176564)
  • Flow Cytometry - Anti-GDNF antibody [EPR2714(N)] (ab176564)
  • Anti-GDNF antibody [EPR2714(N)] (ab176564)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2714(N)] to GDNF
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

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Overview

  • Product name

    Anti-GDNF antibody [EPR2714(N)]
    See all GDNF primary antibodies
  • Description

    Rabbit monoclonal [EPR2714(N)] to GDNF
  • Host species

    Rabbit
  • Specificity

    GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot.
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human GDNF aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P39905

  • Positive control

    • SH-SY5Y and HepG2 whole cell lysate (ab7900), SH-SY5Y cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2714(N)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Growth and Development
    • Neurotrophins
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Other
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Amyotrophic lateral sclerosis
    • Developmental Biology
    • Organogenesis
    • Excretory system development
    • Kidney development

Associated products

  • Alternative Versions

    • Anti-GDNF antibody [EPR2714(N)] - BSA and Azide free (ab249924)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Hep G2 whole cell lysate (ab166833)
  • Recombinant Protein

    • Recombinant human GDNF protein (ab73450)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab176564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/1000 - 1/10000. Predicted molecular weight: 24 kDa.

GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot.

Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 24 kDa.

GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot.

Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
    • Tissue specificity

      In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen.
    • Involvement in disease

      Defects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
      Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia.
    • Sequence similarities

      Belongs to the TGF-beta family. GDNF subfamily.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P39905 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2668 Human
      • Entrez Gene: 14573 Mouse
      • Entrez Gene: 25453 Rat
      • Omim: 600837 Human
      • SwissProt: P39905 Human
      • SwissProt: P48540 Mouse
      • SwissProt: Q07731 Rat
      • Unigene: 248114 Human
      • Unigene: 4679 Mouse
      • Unigene: 53970 Rat
      see all
    • Alternative names

      • Astrocyte derived trophic factor antibody
      • Astrocyte derived trophic factor 1 antibody
      • Astrocyte-derived trophic factor antibody
      • ATF 1 antibody
      • ATF 2 antibody
      • Atf antibody
      • ATF1 antibody
      • ATF2 antibody
      • gdnf antibody
      • GDNF_HUMAN antibody
      • Glial cell derived neurotrophic factor antibody
      • Glial Cell Line Derived Neurotrophic Factor antibody
      • Glial cell line-derived neurotrophic factor antibody
      • Glial derived neurotrophic factor antibody
      • HFB1 GDNF antibody
      • hGDNF antibody
      • HSCR3 antibody
      see all

    Images

    • Western blot - Anti-GDNF antibody [EPR2714(N)] (ab176564)
      Western blot - Anti-GDNF antibody [EPR2714(N)] (ab176564)
      All lanes : Anti-GDNF antibody [EPR2714(N)] (ab176564) at 1/1000 dilution

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 24 kDa

    • Flow Cytometry - Anti-GDNF antibody [EPR2714(N)] (ab176564)
      Flow Cytometry - Anti-GDNF antibody [EPR2714(N)] (ab176564)

      Flow cytometry analysis of permeabilized SH-SY5Y cells labeling GDNF (red) with ab176564 at a 1/10 dilution, or negative control rabbit IgG (green)

    • Anti-GDNF antibody [EPR2714(N)] (ab176564)
      Anti-GDNF antibody [EPR2714(N)] (ab176564)

    Protocols

    • Western blot protocols
    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (9)

    Publishing research using ab176564? Please let us know so that we can cite the reference in this datasheet.

    ab176564 has been referenced in 9 publications.

    • Yang W  et al. Co-overexpression of VEGF and GDNF in adipose-derived stem cells optimizes therapeutic effect in neurogenic erectile dysfunction model. Cell Prolif 53:e12756 (2020). PubMed: 31943490
    • Wang S  et al. GDNFOS1 knockdown decreases the invasion and viability of glioblastoma cells. Exp Ther Med 18:1315-1322 (2019). PubMed: 31316623
    • Wang G  et al. Age-Associated Dopaminergic Neuron Loss and Midbrain Glia Cell Phenotypic Polarization. Neuroscience 415:89-96 (2019). PubMed: 31325563
    • Zheng Z & Liu J GDNF-ADSCs-APG embedding enhances sciatic nerve regeneration after electrical injury in a rat model. J Cell Biochem 120:14971-14985 (2019). PubMed: 31062403
    • Zhang YP  et al. Mifepristone attenuates depression-like changes induced by chronic central administration of interleukin-1ß in rats. Behav Brain Res 347:436-445 (2018). PubMed: 29580890
    • Zhong F  et al. LncRNA NEAT1 promotes colorectal cancer cell proliferation and migration via regulating glial cell-derived neurotrophic factor by sponging miR-196a-5p. Acta Biochim Biophys Sin (Shanghai) 50:1190-1199 (2018). PubMed: 30383193
    • Jia C  et al. Protective effect of compound Danshen (Salvia miltiorrhiza) dripping pills alone and in combination with carbamazepine on kainic acid-induced temporal lobe epilepsy and cognitive impairment in rats. Pharm Biol 56:217-224 (2018). PubMed: 29560767
    • Di G  et al. Corneal Epithelium-Derived Neurotrophic Factors Promote Nerve Regeneration. Invest Ophthalmol Vis Sci 58:4695-4702 (2017). PubMed: 28910445
    • Guo J  et al. Promoting potential of adipose derived stem cells on peripheral nerve regeneration. Mol Med Rep 16:7297-7304 (2017). PubMed: 28944869

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GDNF antibody [EPR2714(N)]

    Excellent
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (C42B Prostate cancer cell line)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    C42B Prostate cancer cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 24 2020

    Western blot abreview for Anti-GDNF antibody [EPR2714(N)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Prostate cancer cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Prostate cancer cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 08 2019

    Western blot abreview for Anti-GDNF antibody [EPR2714(N)]

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (muscle cell line)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    muscle cell line
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 02 2017

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