Overview

  • Product name
    Anti-GDNF antibody [EPR2714(N)]
    See all GDNF primary antibodies
  • Description
    Rabbit monoclonal [EPR2714(N)] to GDNF
  • Host species
    Rabbit
  • Specificity
    GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot.
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GDNF aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P39905

  • Positive control
    • SH-SY5Y and HepG2 whole cell lysate (ab7900), SH-SY5Y cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab176564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 24 kDa.

GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot.

Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
    • Tissue specificity
      In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen.
    • Involvement in disease
      Defects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
      Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia.
    • Sequence similarities
      Belongs to the TGF-beta family. GDNF subfamily.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Astrocyte derived trophic factor antibody
      • Astrocyte derived trophic factor 1 antibody
      • Astrocyte-derived trophic factor antibody
      • ATF 1 antibody
      • ATF 2 antibody
      • Atf antibody
      • ATF1 antibody
      • ATF2 antibody
      • gdnf antibody
      • GDNF_HUMAN antibody
      • Glial cell derived neurotrophic factor antibody
      • Glial Cell Line Derived Neurotrophic Factor antibody
      • Glial cell line-derived neurotrophic factor antibody
      • Glial derived neurotrophic factor antibody
      • HFB1 GDNF antibody
      • hGDNF antibody
      • HSCR3 antibody
      see all

    Images

    • All lanes : Anti-GDNF antibody [EPR2714(N)] (ab176564) at 1/1000 dilution

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 24 kDa

    • Flow cytometry analysis of permeabilized SH-SY5Y cells labeling GDNF (red) with ab176564 at a 1/10 dilution, or negative control rabbit IgG (green)

    References

    This product has been referenced in:
    • Zhang YP  et al. Mifepristone attenuates depression-like changes induced by chronic central administration of interleukin-1ß in rats. Behav Brain Res 347:436-445 (2018). Read more (PubMed: 29580890) »
    • Di G  et al. Corneal Epithelium-Derived Neurotrophic Factors Promote Nerve Regeneration. Invest Ophthalmol Vis Sci 58:4695-4702 (2017). Read more (PubMed: 28910445) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (muscle cell line)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    muscle cell line
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C

    Abcam user community

    Verified customer

    Submitted Feb 02 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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