Western blot abreview for Anti-GDNF antibody [EPR2714(N)]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (muscle cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
muscle cell line
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 27°C
Dilution
1/2000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: HRP goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 25 kDa

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Submitted Feb 02 2017

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