Anti-GDNF Receptor alpha 1/GFRA1 antibody (ab205372)
Key features and details
- Chicken polyclonal to GDNF Receptor alpha 1/GFRA1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgY
Overview
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Product name
Anti-GDNF Receptor alpha 1/GFRA1 antibody
See all GDNF Receptor alpha 1/GFRA1 primary antibodies -
Description
Chicken polyclonal to GDNF Receptor alpha 1/GFRA1 -
Host species
Chicken -
Specificity
ab205372 doesn’t cross-react with hGFRa-2, hGFRa-3, hGFRa-4 -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein (His-tag) corresponding to Human GDNF Receptor alpha 1/GFRA1. Produced using CHO-based proprietary suspension cell line. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Database link: P56159 -
Positive control
- U2OS cells; His tagged GDNF Receptor alpha 1/GFRA1.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS
As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab205372 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.5 - 1 µg/ml.
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WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 51 kDa.
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Notes |
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ICC/IF
Use a concentration of 0.5 - 1 µg/ml. |
WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 51 kDa. |
Target
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Function
Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor. -
Sequence similarities
Belongs to the GDNFR family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2674 Human
- Omim: 601496 Human
- SwissProt: P56159 Human
- Unigene: 388347 Human
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Alternative names
- GDNF family receptor alpha 1 antibody
- GDNF family receptor alpha-1 antibody
- GDNF R antibody
see all
Images
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Immunofluorescent analysis of U2OS cells labeling GDNF Receptor alpha 1/GFRA1 using ab205372 at 1/2000. Goat anti-chicken IgY - H&L DyLight 550 (ab96952) at 1/200 dilution was used as secondary antibody. For nuclear staining DAPI was used.
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Anti-GDNF Receptor alpha 1/GFRA1 antibody (ab205372) at 1/5000 dilution + His tagged GDNF Receptor alpha 1/GFRA1 at 0.1 µg
Secondary
Rabbit anti-chicken antibody
Predicted band size: 51 kDa
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab205372 has not yet been referenced specifically in any publications.