Key features and details
- Rabbit polyclonal to GDNF Receptor alpha 1/GFRA1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-GDNF Receptor alpha 1/GFRA1 antibody
See all GDNF Receptor alpha 1/GFRA1 primary antibodies
DescriptionRabbit polyclonal to GDNF Receptor alpha 1/GFRA1
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human GDNF Receptor alpha 1/GFRA1 aa 369-382.
(Peptide available as
- Human brain lysate.
This product was previously labelled as GDNF Receptor alpha 1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab8026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immunohistochemistry (PFA fixed): 1/50.
WB: 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionReceptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor.
Sequence similaritiesBelongs to the GDNFR family.
Cellular localizationCell membrane.
- Information by UniProt
- GDNF family receptor alpha 1 antibody
- GDNF family receptor alpha-1 antibody
- GDNF R antibody
Immunohistochemistry of human brain tissue staining GDNF Receptor alpha 1/GFRA1 using ab8026 at 2.5 μg/ml.
Immunocytochemistry of human brain tissue staining GDNF Receptor alpha 1/GFRA1 using ab8026 at 20 μg/ml.
All lanes : Anti-GDNF Receptor alpha 1/GFRA1 antibody (ab8026) at 1/500 dilution
Lane 1 : Crude membrane fractions of human brain
Lane 2 : Crude membrane fractions of liver
Lane 3 : Crude membrane fractions of kidney
Lane 4 : Crude membrane fractions of spleen
Predicted band size: 50 kDa
Observed band size: 50 kDa
Western blot analysis of GFR alpha 1/GFRA1 in crude membrane fractions of human brain (B), liver (L), kidney (K), and spleen (S), respectively, with ab8026 at 1/500 dilution.
Immunohistochemistry of human brain tissue staining GDNF Receptor alpha 1/GFRA1 using ab8026 at 1 μg/ml.
ab8026 has been referenced in 9 publications.
- Jung SE et al. Effect of Equilibration Time and Temperature on Murine Spermatogonial Stem Cell Cryopreservation. Biopreserv Biobank 18:213-221 (2020). PubMed: 32216643
- Rice FL et al. The evolution and multi-molecular properties of NF1 cutaneous neurofibromas originating from C-fiber sensory endings and terminal Schwann cells at normal sites of sensory terminations in the skin. PLoS One 14:e0216527 (2019). PubMed: 31107888
- Wang H et al. Transcriptional regulation of P63 on the apoptosis of male germ cells and three stages of spermatogenesis in mice. Cell Death Dis 9:76 (2018). PubMed: 29362488
- Fu H et al. PAK1 Promotes the Proliferation and Inhibits Apoptosis of Human Spermatogonial Stem Cells via PDK1/KDR/ZNF367 and ERK1/2 and AKT Pathways. Mol Ther Nucleic Acids 12:769-786 (2018). PubMed: 30141410
- Gat I et al. Initial germ cell to somatic cell ratio impacts the efficiency of SSC expansion in vitro. Syst Biol Reprod Med 64:39-50 (2018). PubMed: 29193985
- Harandi VM et al. Unchanged Neurotrophic Factors and Their Receptors Correlate With Sparing in Extraocular Muscles in Amyotrophic Lateral Sclerosis. Invest Ophthalmol Vis Sci 57:6831-6842 (2016). PubMed: 28002846
- Bellaïche J et al. Gdnf-Gfra1 pathway is expressed in a spermatogenetic-dependent manner and is regulated by fsh in a fish testis. Biol Reprod 91:94 (2014). PubMed: 25165121
- He Z et al. Isolation, characterization, and culture of human spermatogonia. Biol Reprod 82:363-72 (2010). IHC-P ; Human . PubMed: 19846602
- Serra MP et al. Ret, GFRalpha-1, GFRalpha-2 and GFRalpha-3 receptors in the human hippocampus and fascia dentata. Int J Dev Neurosci 23:425-38 (2005). WB, IHC-P . PubMed: 16002253