Overview

  • Product name

    Anti-GDNF Receptor alpha 1/GFRA1 antibody
    See all GDNF Receptor alpha 1/GFRA1 primary antibodies
  • Description

    Rabbit polyclonal to GDNF Receptor alpha 1/GFRA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human GDNF Receptor alpha 1/GFRA1 aa 150-250 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab93754)

  • Positive control

    • This antibody gave a positive signal in Human Brain Tissue Lysate and Brain Tissue Membrane Extract. This antibody gave a positive result in IHC in the following FFPE tissue: Human kidney cancer.
  • General notes

     This product was previously labelled as GDNF Receptor alpha 1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IP Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor.
  • Sequence similarities

    Belongs to the GDNFR family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • GDNF family receptor alpha 1 antibody
    • GDNF family receptor alpha-1 antibody
    • GDNF R antibody
    • GDNF RA antibody
    • GDNF receptor alpha antibody
    • GDNF receptor alpha-1 antibody
    • GDNFR alpha 1 antibody
    • GDNFR alpha antibody
    • GDNFR antibody
    • GDNFR-alpha-1 antibody
    • GDNFRA antibody
    • GDNFRalpha antibody
    • GFR alpha 1 antibody
    • GFR alpha1 antibody
    • GFR-alpha-1 antibody
    • GFRA 1 antibody
    • Gfra1 antibody
    • GFRA1_HUMAN antibody
    • GFRalpha1 antibody
    • Glial cell line derived neurotrophic factor receptor alpha antibody
    • GPI linked anchor protein antibody
    • MGC23045 antibody
    • PI linked cell surface accessory protein antibody
    • RET 1L antibody
    • RET ligand 1 antibody
    • RET1L antibody
    • RETL 1 antibody
    • RETL1 antibody
    • TGF beta related neurotrophic factor receptor 1 antibody
    • TGF-beta-related neurotrophic factor receptor 1 antibody
    • TRNR 1 antibody
    • TRNR1 antibody
    see all

Images

  • IHC image of GDNF Receptor alpha 1/GFRA1 staining in Human kidney cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84106, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • GDNF Receptor alpha 1/GFRA1 was immunoprecipitated using 0.5mg Mouse Brain tissue, 5µg of Rabbit polyclonal to GDNF Receptor alpha 1/GFRA1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab84106.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 51kDa; GDNF Receptor alpha 1/GFRA1, non specific - as present in control (lane 2); We are confident this was due to slight lane contamination and the band seen in the IP lane is our target of interest.

  • All lanes : Anti-GDNF Receptor alpha 1/GFRA1 antibody (ab84106) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain Tissue Membrane Extract

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 51 kDa
    Observed band size: 51 kDa


    Exposure time: 16 minutes
  • ICC/IF image of ab84106 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84106, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Shlush E  et al. In vitro generation of Sertoli-like and haploid spermatid-like cells from human umbilical cord perivascular cells. Stem Cell Res Ther 8:37 (2017). Read more (PubMed: 28202061) »
  • Wang H  et al. Induction of Germ Cell-like Cells from Porcine Induced Pluripotent Stem Cells. Sci Rep 6:27256 (2016). ICC/IF ; Pig . Read more (PubMed: 27264660) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Sample
Spotted Catshark (Scyliorhinus canicula) Tissue sections (testis)
Specification
testis
Permeabilization
Yes - 0.1% triton/1% BSA/PBS
Fixative
Paraformaldehyde

Ms. Aude Gautier

Verified customer

Submitted Mar 31 2015

Answer

Thank you for the sequence information.


I ran Clustal W alignment analysis of the 3 isoforms and the antibody immunogen sequence. The results (attach file) show that the antibody has 100 score value with isoform 1a (Name: one) and isoform 1c (Name: three). However, the results also show similarities between isoform 1a (Name: one) and isoform 1b (Name: two) at 95,isoform 1a and isoform 1cat 80. I am not sure what kind of non specificity you are trying to avoid but I hope this information will be helpful.


Have a nice day!

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