Overview

  • Product name
    Anti-GDNF Receptor alpha 2 antibody
    See all GDNF Receptor alpha 2 primary antibodies
  • Description
    Rabbit polyclonal to GDNF Receptor alpha 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC (PFA fixed)more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Peptide corresponding to amino acids 377 to 391of human GFRa-2. The amino acid sequences ofimmunogenic peptide are identical to those of mouse andrat.

  • Positive control
    • HeLa whole cell lysate
  • General notes

    Members of the glial cell line-derived neurotrophic factor(GDNF) family, including GDNF and neurturin (NTN),play key roles in the control of vertebrate neuron survivaland differentiation. Physiological responses to NTN requirethe presence of a novel glycosylphosphadidylinositollinked protein NTNRa, which is a cell surface receptor forNTN. The cDNAs encoding NTNRa from human, rat,chicken, and mouse have been cloned recently. NTNRa was also termed GDNFRb, Ret ligand 2 (RETL2)or TGF-b-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFRa-2 recently. GFRa-2 binds NTN and mediates activation of RET receptor tyrosine kinase by both NTN and GDNF. Thus, NTN, GFRa-2, and the Ret PTK form a complex to transduce NTN signal andto mediate NTN function.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity
    Ion Exchange Chromatography
  • Primary antibody notes
    Members of the glial cell line-derived neurotrophic factor(GDNF) family, including GDNF and neurturin (NTN),play key roles in the control of vertebrate neuron survivaland differentiation. Physiological responses to NTN requirethe presence of a novel glycosylphosphadidylinositollinked protein NTNRa, which is a cell surface receptor forNTN. The cDNAs encoding NTNRa from human, rat,chicken, and mouse have been cloned recently. NTNRa was also termed GDNFRb, Ret ligand 2 (RETL2)or TGF-b-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFRa-2 recently. GFRa-2 binds NTN and mediates activation of RET receptor tyrosine kinase by both NTN and GDNF. Thus, NTN, GFRa-2, and the Ret PTK form a complex to transduce NTN signal andto mediate NTN function.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use at an assay dependent concentration. Can be blocked with Human GDNF Receptor alpha 2 peptide (ab39769).
IHC (PFA fixed) 1/50.

Target

  • Function
    Receptor for neurturin. Mediates the NRTN-induced autophosphorylation and activation of the RET receptor. Also able to mediate GDNF signaling through the RET tyrosine kinase receptor.
    Isoform 2: Participates to NRTN-induced 'Ser-727' phosphorylation of STAT3.
  • Tissue specificity
    Isoform 1 is found in both brain and placenta.
  • Sequence similarities
    Belongs to the GDNFR family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GDNF family receptor alpha 2 antibody
    • GDNF family receptor alpha-2 antibody
    • GDNF receptor alpha-2 antibody
    • GDNF receptor beta antibody
    • GDNFR beta antibody
    • GDNFR-alpha-2 antibody
    • GDNFR-beta antibody
    • GDNFRB antibody
    • GFR alpha 2 antibody
    • GFR-alpha-2 antibody
    • GFRA 2 antibody
    • Gfra2 antibody
    • GFRA2_HUMAN antibody
    • Glial cell line derived neurotrophic factor family receptor alpha2b antibody
    • Glial cell line derived neurotrophic factor receptor beta antibody
    • Neurturin receptor alpha antibody
    • NRTNR alpha antibody
    • NRTNR-alpha antibody
    • NTNR alpha antibody
    • NTNR-alpha antibody
    • NTNRA antibody
    • PI linked cell surface accessory protein antibody
    • PI linked cell-surface accessory protein antibody
    • RET ligand 2 antibody
    • RETL 2 antibody
    • RETL2 antibody
    • TGF beta related neurotrophic factor receptor 2 antibody
    • TGF-beta-related neurotrophic factor receptor 2 antibody
    • TRN receptor GPI anchored antibody
    • TRNR 2 antibody
    • TRNR2 antibody
    see all

Images

  • Anti-GDNF Receptor alpha 2 antibody (ab8027) at 1/1000 dilution + HeLa total cell lysate

    Observed band size: 52 kDa
    why is the actual band size different from the predicted?

  • ab8027 at 5µg/ml staining GDNF Receptor alpha2 in Hela cells by ICC/IF.

References

This product has been referenced in:
  • Hai B  et al. Transient activation of hedgehog pathway rescued irradiation-induced hyposalivation by preserving salivary stem/progenitor cells and parasympathetic innervation. Clin Cancer Res 20:140-50 (2014). Read more (PubMed: 24150232) »
  • Wang K  et al. The neurotrophic factor neurturin contributes toward an aggressive cancer cell phenotype, neuropathic pain and neuronal plasticity in pancreatic cancer. Carcinogenesis N/A:N/A (2013). IHC ; Human . Read more (PubMed: 24067900) »
See all 3 Publications for this product

Customer reviews and Q&As

Answer

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee forWB and for mouse, rat and human samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

In particular it would be helpful to receive further information on the purified protein that you are using. Is this a recombinant protein, was this full length protein? Or is it purified endogenous native protein? Has it been reduced and denatured?

It is possible that this antibody is not quite as sensitive as the other one you are using and you may need to load more protein for detection.

For the blot with which you used ab8027, could you confirm the quality of the sampleand transfer to the membrane from that lane was checked using commassie on ponceu red staining?

Which species was the purified protein sample from?

Thank you for your time and cooperation. We look forward to receiving the completed questionnaire and further information.

Order Details
Antibody code:

Problem
Choose: Non-specific band Multiple bands No signal or weak signal High background

Lot number

Purchase order number
or preferably Abcam order number:



General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, wrong band size, more bands, no band etc.)


Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)


Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)


Amount of protein loaded


loaded 60ug of cell membrane fraction protein in lanes 1 and 3 and in lanes 2 and 4 I have loaded 0.06ug of pure GFRa2 protein as a positive control

Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)


Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)

1% milk/PBS over night @1:500 dilution @ 4 degrees C.


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)

secondary rabbit antibody was added @1:5000 in 1% milk/PBS at room temp for 1 hour


Detection method (ECL, ECLPlus etc.)


Positive and negative controls used (please specify)



Optimization attempts (problem solving)
How many times have you tried the Western?



Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No
e.g. are the background bands always in the same place?


What steps have you altered?


Additional Notes:


Image:
We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asess the results.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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