Anti-GDNF Receptor alpha 2/GFRA2 antibody (ab205351)
Key features and details
- Chicken polyclonal to GDNF Receptor alpha 2/GFRA2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgY
Overview
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Product name
Anti-GDNF Receptor alpha 2/GFRA2 antibody
See all GDNF Receptor alpha 2/GFRA2 primary antibodies -
Description
Chicken polyclonal to GDNF Receptor alpha 2/GFRA2 -
Host species
Chicken -
Specificity
ab205351 does not recognizes hGFRa-1, hGFRa-3, hGFRa-4. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein (His-tag) corresponding to Human GDNF Receptor alpha 2/GFRA2. Produced using CHO suspension cell line, and then purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Database link: O00451 -
Positive control
- U2OS cells; His tagged GDNF Receptor alpha 2/GFRA2 recombinant protein.
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General notes
This product was previously labelled as GDNF Receptor alpha 2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS
As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab205351 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 51 kDa. | |
| ICC/IF | Use a concentration of 1 - 2 µg/ml. |
Target
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Function
Receptor for neurturin. Mediates the NRTN-induced autophosphorylation and activation of the RET receptor. Also able to mediate GDNF signaling through the RET tyrosine kinase receptor.
Isoform 2: Participates to NRTN-induced 'Ser-727' phosphorylation of STAT3. -
Tissue specificity
Isoform 1 is found in both brain and placenta. -
Sequence similarities
Belongs to the GDNFR family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2675 Human
- Omim: 601956 Human
- SwissProt: O00451 Human
- Unigene: 441202 Human
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Alternative names
- GDNF family receptor alpha 2 antibody
- GDNF family receptor alpha-2 antibody
- GDNF receptor alpha-2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GDNF Receptor alpha 2/GFRA2 antibody (ab205351)
Immunofluorescent analysis of U2OS cells labeling GDNF Receptor alpha 2/GFRA2 using ab205351 at 1/2000. Goat anti-chicken IgY - H&L DyLight 550 (ab96952) at 1/200 dilution was used as secondary antibody. For nuclear staining DAPI was used.
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Western blot - Anti-GDNF Receptor alpha 2/GFRA2 antibody (ab205351)Anti-GDNF Receptor alpha 2/GFRA2 antibody (ab205351) at 5000 µg/ml + His tagged GDNF Receptor alpha 2/GFRA2 at 0.1 µg
Secondary
Rabbit anti-chicken
Predicted band size: 51 kDa
Protocols
Datasheets and documents
References (0)
ab205351 has not yet been referenced specifically in any publications.
