Key features and details
- Chicken polyclonal to GDNF Receptor alpha 2/GFRA2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgY
Product nameAnti-GDNF Receptor alpha 2/GFRA2 antibody
See all GDNF Receptor alpha 2/GFRA2 primary antibodies
DescriptionChicken polyclonal to GDNF Receptor alpha 2/GFRA2
Specificityab205351 does not recognizes hGFRa-1, hGFRa-3, hGFRa-4.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant full length protein (His-tag) corresponding to Human GDNF Receptor alpha 2/GFRA2. Produced using CHO suspension cell line, and then purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Database link: O00451
- U2OS cells; His tagged GDNF Receptor alpha 2/GFRA2 recombinant protein.
This product was previously labelled as GDNF Receptor alpha 2
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituent: 100% PBS
As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab205351 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 51 kDa.|
|ICC/IF||Use a concentration of 1 - 2 µg/ml.|
FunctionReceptor for neurturin. Mediates the NRTN-induced autophosphorylation and activation of the RET receptor. Also able to mediate GDNF signaling through the RET tyrosine kinase receptor.
Isoform 2: Participates to NRTN-induced 'Ser-727' phosphorylation of STAT3.
Tissue specificityIsoform 1 is found in both brain and placenta.
Sequence similaritiesBelongs to the GDNFR family.
Cellular localizationCell membrane.
- Information by UniProt
- GDNF family receptor alpha 2 antibody
- GDNF family receptor alpha-2 antibody
- GDNF receptor alpha-2 antibody
Immunofluorescent analysis of U2OS cells labeling GDNF Receptor alpha 2/GFRA2 using ab205351 at 1/2000. Goat anti-chicken IgY - H&L DyLight 550 (ab96952) at 1/200 dilution was used as secondary antibody. For nuclear staining DAPI was used.
Anti-GDNF Receptor alpha 2/GFRA2 antibody (ab205351) at 5000 µg/ml + His tagged GDNF Receptor alpha 2/GFRA2 at 0.1 µg
Predicted band size: 51 kDa
ab205351 has not yet been referenced specifically in any publications.