Anti-GDNF Receptor alpha 2/GFRA2 antibody (ab205351)
- Datasheet
- References
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Overview
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Product name
Anti-GDNF Receptor alpha 2/GFRA2 antibody
See all GDNF Receptor alpha 2/GFRA2 primary antibodies -
Description
Chicken polyclonal to GDNF Receptor alpha 2/GFRA2 -
Host species
Chicken -
Specificity
ab205351 does not recognizes hGFRa-1, hGFRa-3, hGFRa-4. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein (His-tag) corresponding to Human GDNF Receptor alpha 2/GFRA2. Produced using CHO suspension cell line, and then purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Database link: O00451 -
Positive control
- U2OS cells; His tagged GDNF Receptor alpha 2/GFRA2 recombinant protein.
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General notes
This product was previously labelled as GDNF Receptor alpha 2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS
As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab205351 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 51 kDa. | |
| ICC/IF | Use a concentration of 1 - 2 µg/ml. |
Target
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Function
Receptor for neurturin. Mediates the NRTN-induced autophosphorylation and activation of the RET receptor. Also able to mediate GDNF signaling through the RET tyrosine kinase receptor.
Isoform 2: Participates to NRTN-induced 'Ser-727' phosphorylation of STAT3. -
Tissue specificity
Isoform 1 is found in both brain and placenta. -
Sequence similarities
Belongs to the GDNFR family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2675 Human
- Omim: 601956 Human
- SwissProt: O00451 Human
- Unigene: 441202 Human
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Alternative names
- GDNF family receptor alpha 2 antibody
- GDNF family receptor alpha-2 antibody
- GDNF receptor alpha-2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GDNF Receptor alpha 2/GFRA2 antibody (ab205351)Immunofluorescent analysis of U2OS cells labeling GDNF Receptor alpha 2/GFRA2 using ab205351 at 1/2000. Goat anti-chicken IgY - H&L DyLight 550 (ab96952) at 1/200 dilution was used as secondary antibody. For nuclear staining DAPI was used.
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Western blot - Anti-GDNF Receptor alpha 2/GFRA2 antibody (ab205351)Anti-GDNF Receptor alpha 2/GFRA2 antibody (ab205351) at 5000 µg/ml + His tagged GDNF Receptor alpha 2/GFRA2 at 0.1 µg
Secondary
Rabbit anti-chicken
Predicted band size: 51 kDa
Datasheets and documents
References
ab205351 has not yet been referenced specifically in any publications.
