Anti-GDX antibody (ab241427)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-GDX antibody
See all GDX primary antibodies -
Description
Rabbit polyclonal to GDX -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Orangutan
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Immunogen
Synthetic peptide within Human GDX aa 25-75. The exact sequence is proprietary.
Database link: P11441 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to GDX immobilized on solid support -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab241427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. |
Application notes
Is unsuitable for IP.
Target
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Function
Component of the BAT3 complex, a multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. The complex acts by facilitating TA proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins, and transfers them to ASNA1/TRC40 for targeting. -
Sequence similarities
Contains 1 ubiquitin-like domain. -
Cellular localization
Cytoplasm > cytosol. - Information by UniProt
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Database links
- Entrez Gene: 8266 Human
- Entrez Gene: 27643 Mouse
- Entrez Gene: 100173917 Orangutan
- Entrez Gene: 100328764 Rabbit
- Omim: 312070 Human
- SwissProt: P11441 Human
- SwissProt: P21126 Mouse
- SwissProt: Q5R4T1 Orangutan
see all -
Alternative names
- DX254E antibody
- DXS254E antibody
- G6PD antibody
see all
Images
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Western blot - Anti-GDX antibody (ab241427)All lanes : Anti-GDX antibody (ab241427) at 0.4 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Exposure time: 30 secondsPrepared using NETN lysis buffer.
Datasheets and documents
References
ab241427 has not yet been referenced specifically in any publications.
