Overview

  • Product name

  • Description

    Rabbit polyclonal to GEF H1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 625-675 of Human GEF H1 (CAH72629.1).

  • Positive control

    • Human stomach adenocarcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab103558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.

Target

  • Function

    Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in PubMed:9857026. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIP2 dependent NF-kappaB signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides through NOD1 that is independent of its GEF activity, but also in the activation of NF-kappaB by Shigella effector proteins (IpgB2 and OspB) which requires its GEF activity and the activation of RhoA.
  • Sequence similarities

    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
  • Domain

    The DH (DBL-homology) domain interacts with and promotes loading of GTP on RhoA.
    The PH (pleckstrin-homology) domain is involved in microtubule binding and targeting to tight junctions.
  • Post-translational
    modifications

    Phosphorylation of Ser-886 by PAK1 induces binding to protein 14-3-3 zeta, promoting its relocation to microtubules and the inhibition of its activity. Phosphorylated by STK6 and CDK1 during mitosis, which negatively regulates its activity. Phosphorylation by MAPK1 or MAPK3 increases nucleotide exchange activity. Phosphorylation by PAK4 releases GEF-H1 from the microtubules.
  • Cellular localization

    Cytoplasm. Cell junction > tight junction. Golgi apparatus. Cytoplasm > cytoskeleton > spindle. Cell projection > ruffle membrane. Localizes to the tips of cortical microtubules of the mitotic spindle during cell division, and is further released upon microtubule depolymerization. Recruited into membrane ruffles induced by S.flexneri at tight junctions of polarized epithelial cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • AA408978 antibody
    • ARHG2 antibody
    • ARHG2_HUMAN antibody
    • ARHGEF 2 antibody
    • ARHGEF-2 antibody
    • ARHGEF2 antibody
    • GEF antibody
    • GEF H1 antibody
    • GEF-H1 antibody
    • GEFH1 antibody
    • Guanine nucleotide exchange factor H1 antibody
    • KIAA0651 antibody
    • Lbcl1 antibody
    • Lfc antibody
    • LFP40 antibody
    • MGC95068 antibody
    • Microtubule-regulated Rho-GEF antibody
    • mKIAA0651 antibody
    • P40 antibody
    • Proliferating cell nucleolar antigen p40 antibody
    • Protein GEF-H1 antibody
    • Rho guanine nucleotide exchange factor 2 antibody
    • rho/rac guanine nucleotide exchange factor (GEF) 2 antibody
    • rho/rac guanine nucleotide exchange factor 2 antibody
    • rho/rac guanine nucleotide exchange factor antibody
    see all

Images

  • Formalin-fixed paraffin-embedded section of Human stomach adenocarcinoma. ab103558 used at a dilution of 1/250. Detection: DAB staining.

References

This product has been referenced in:

  • Schossleitner K  et al. Evidence That Cingulin Regulates Endothelial Barrier Function In Vitro and In Vivo. Arterioscler Thromb Vasc Biol 36:647-54 (2016). Read more (PubMed: 26821949) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

As requested, I have issued a free of charge replacement with one unit of ab103558. The order number is ********.
To check the status of the order please contact our Customer Service team and reference this number.
I wish you the best of luck with your research.

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Answer

Thank you for your reply and your patience. I have contacted the laboratory producing the ab103558 in regards to the recognition of the phospho and the non-phospho form. The lab replied that there is a residue which can be reportedly phosphorylated within the epitope. Unfortunately we have no data upon which to judge the relative efficiency of binding of the antibody to the phosphorylated vs non-phosphorylated epitope. However as the immunogen is different from the immunogen of the ab90783, there might not be the same pattern? The immunogen for ab103558 is region between residue 625-675. We do not have small aliquots which we could provide for testing purposes. However I can make you a testing offer, if you would need to test this antibody also on another application or species? For example on frozen section immunohistochemistry? The offer would consist of a discount of a future antibody purchase after you would have provided the feedback. The discount would be 100%. Please do let me know if you would be interested, as I would need to provide you a code. I am sorry for not being more helpful at the moment. Please let me know if I could help you otherwise or if you would like to discuss the results of ab90783. I am looking forward to hear back from you and wish you meanwhile also a very merry Christmas time!

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