Overview

  • Product name
  • Description
    Rabbit polyclonal to GEF H1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 656-1000 of Human GEF H1.

  • Positive control
    • 293T, A431, HeLa, hMSC-3A6, RAW264.7 and C2C12 whole cell lysates; GEF H1 protein; HeLa cells; Human gastric carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/500 - 1/3000. Predicted molecular weight: 111 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in PubMed:9857026. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIP2 dependent NF-kappaB signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides through NOD1 that is independent of its GEF activity, but also in the activation of NF-kappaB by Shigella effector proteins (IpgB2 and OspB) which requires its GEF activity and the activation of RhoA.
  • Sequence similarities
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
  • Domain
    The DH (DBL-homology) domain interacts with and promotes loading of GTP on RhoA.
    The PH (pleckstrin-homology) domain is involved in microtubule binding and targeting to tight junctions.
  • Post-translational
    modifications
    Phosphorylation of Ser-886 by PAK1 induces binding to protein 14-3-3 zeta, promoting its relocation to microtubules and the inhibition of its activity. Phosphorylated by STK6 and CDK1 during mitosis, which negatively regulates its activity. Phosphorylation by MAPK1 or MAPK3 increases nucleotide exchange activity. Phosphorylation by PAK4 releases GEF-H1 from the microtubules.
  • Cellular localization
    Cytoplasm. Cell junction > tight junction. Golgi apparatus. Cytoplasm > cytoskeleton > spindle. Cell projection > ruffle membrane. Localizes to the tips of cortical microtubules of the mitotic spindle during cell division, and is further released upon microtubule depolymerization. Recruited into membrane ruffles induced by S.flexneri at tight junctions of polarized epithelial cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA408978 antibody
    • ARHG2 antibody
    • ARHG2_HUMAN antibody
    • ARHGEF 2 antibody
    • ARHGEF-2 antibody
    • ARHGEF2 antibody
    • GEF antibody
    • GEF H1 antibody
    • GEF-H1 antibody
    • GEFH1 antibody
    • Guanine nucleotide exchange factor H1 antibody
    • KIAA0651 antibody
    • Lbcl1 antibody
    • Lfc antibody
    • LFP40 antibody
    • MGC95068 antibody
    • Microtubule-regulated Rho-GEF antibody
    • mKIAA0651 antibody
    • P40 antibody
    • Proliferating cell nucleolar antigen p40 antibody
    • Protein GEF-H1 antibody
    • Rho guanine nucleotide exchange factor 2 antibody
    • rho/rac guanine nucleotide exchange factor (GEF) 2 antibody
    • rho/rac guanine nucleotide exchange factor 2 antibody
    • rho/rac guanine nucleotide exchange factor antibody
    see all

Images

  • Immunocytochemical immunofluorescence analysis of formaldehyde-fixed human HepG2 cells, labelling GEF H1 with ab155785 with a dilution of 1/50 incubated for 3 hours at 22°C in 1% donkey serum in 0.1% PBST diluent. Permeablization with 0.2% Triton X-100. Blocking was with 1% donkey serum in 0.1% PBST incubated for 1 hour at 22°C. Secondary was a donkey anti-rabbit polyclonal Alexa Fluor® 594 at 1/200. Counterstain is DAPI (blue) against nuclear DNA.

    See Abreview

  • All lanes : Anti-GEF H1 antibody (ab155785) at 1/2000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 111 kDa



    5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human Gastric carcinoma tissue, labeling GEF H1 with ab155785 at 1/500 dilution.
  • Immunoprecipitation of GEF H1 from 293T whole cell extracts using 5 μg of ab155785. Western blot analysis was performed using ab155785.

  • Immunohistochemical analysis of paraffin-embedded Mouse testis tissue, labeling GEF H1 with ab155785 at 1/500 dilution.

  • All lanes : Anti-GEF H1 antibody (ab155785) at 1/1000 dilution

    Lane 1 : hMSC-3A6 whole cell lysate, mock transfected
    Lane 2 : hMSC-3A6 whole cell lysate, transfected with GEF H1 siRNA

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 111 kDa



    8% SDS PAGE
  • All lanes : Anti-GEF H1 antibody (ab155785) at 1/1000 dilution

    Lane 1 : RAW264.7 whole cell lysate
    Lane 2 : C2C12 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 111 kDa



    5 % SDS-PAGE
  • Immunofluorescent analysis of methanol-fixed (-20°C 100% MeOH for 5 min) HeLa cells, labeling GEF H1 with ab155785 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.

References

This product has been referenced in:
  • Jiu Y  et al. Vimentin intermediate filaments control actin stress fiber assembly through GEF-H1 and RhoA. J Cell Sci 130:892-902 (2017). Read more (PubMed: 28096473) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Jurkat T Cell Line)
Total protein in input
1000 µg
Immuno-precipitation step
Protein A
Specification
Jurkat T Cell Line

Dr. Jeffrey Johnson

Verified customer

Submitted Sep 19 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (HT-1080 cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
5 µg
Specification
HT-1080 cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Feb 01 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2 cells)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
25 µg
Specification
HepG2 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Oct 09 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HepG2 cells)
Permeabilization
Yes - 0.2% Triton X-100
Specification
HepG2 cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Oct 09 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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