Overview

  • Product name
    Anti-GEFT antibody
  • Description
    Rabbit polyclonal to GEFT
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to region within amino acids 226-446 of Human GEFT.

  • Positive control
    • NT2D1, IMR32, U-87 MG and MCF7 whole cell lysates; Human breast carcinoma tissue.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab127690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 64 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. Links specifically G alpha q/11-coupled receptors to RHOA activation. May be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. Involved in skeletal myogenesis.
  • Tissue specificity
    Isoform 1 and isoform 2 are highly expressed in excitable tissues, such as brain, heart and muscle. Also detected in kidney and liver.
  • Sequence similarities
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
  • Domain
    The guanine nucleotide exchange activity is autoinhibited by the PH domain.
  • Cellular localization
    Cell membrane. Cytoplasm > myofibril > sarcomere. Highly colocalizes with actin regions.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARHGEF25 antibody
    • ARHGP_HUMAN antibody
    • GEFT antibody
    • Guanine nucleotide exchange factor GEFT antibody
    • p63RhoGEF antibody
    • RAC/CDC42 exchange factor antibody
    • Rac/Cdc42/Rho exchange factor GEFT antibody
    • Rho guanine nucleotide exchange factor 25 antibody
    • RhoA/RAC/CDC42 exchange factor antibody
    • RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT antibody
    see all

Images

  • Anti-GEFT antibody (ab127690) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg

    Predicted band size: 64 kDa



    10% SDS PAGE
  • ab127690, at a dilution of 1/250, staining GEFT in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.

References

ab127690 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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