Recombinant Anti-Gelsolin antibody [2/Gelsolin] (ab303676)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [2/Gelsolin] to Gelsolin
- Suitable for: Indirect ELISA, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Gelsolin antibody [2/Gelsolin]
See all Gelsolin primary antibodies -
Description
Mouse monoclonal [2/Gelsolin] to Gelsolin -
Host species
Mouse -
Tested applications
Suitable for: Indirect ELISA, WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB : THP-1 cell culture supernatant. THP-1 and MCF7 whole cell lysate. Human plasma. ELISA (Indirect): Human gelsolin.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
2/Gelsolin -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab303676 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Indirect ELISA |
1/1000.
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WB |
1/1000. Predicted molecular weight: 86 kDa.
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Notes |
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Indirect ELISA
1/1000. |
WB
1/1000. Predicted molecular weight: 86 kDa. |
Target
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Function
Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis. -
Tissue specificity
Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells. -
Involvement in disease
Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure. -
Sequence similarities
Belongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats. -
Post-translational
modificationsPhosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids. -
Cellular localization
Cytoplasm > cytoskeleton and Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2934 Human
- Omim: 137350 Human
- SwissProt: P06396 Human
- Unigene: 522373 Human
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Alternative names
- Actin depolymerizing factor antibody
- Actin-depolymerizing factor antibody
- ADF antibody
see all
Images
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All lanes : Anti-Gelsolin antibody [2/Gelsolin] (ab303676) at 1/1000 dilution
Lane 1 : THP-1 (human monocytic leukemia monocyte) cell culture supernatant at 10 µl
Lane 2 : THP-1 (human monocytic leukemia monocyte), whole cell lysate at 10 µg
Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 10 µg
Lane 4 : Human plasma at 10 µg
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 86 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The secretory isoform of gelsolin is at 86kDa.
The cytoplasmic isoforms of gelsolin are at 81kDa according to the Uniprot.
Exposure time:
Lanes 1: 48 seconds
Lanes 2-4: 10 seconds -
Indirect ELISA - Anti-Gelsolin antibody [2/Gelsolin] (ab303676)
Antigen used at 1000 ng/ml.
AB303676 used at 0-2000 ng/ml.
Secondary: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) used at a 1/1000 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab303676 has not yet been referenced specifically in any publications.