Product nameAnti-Gelsolin antibody
See all Gelsolin primary antibodies
DescriptionAlpaca monoclonal to Gelsolin
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human Gelsolin.
Database link: P06396
Contains a FLAG tag (DDDDK) and a His tag Therefore, an anti DDDDK or anti His secondary can be used. We recommend the anti-FLAG option (ab49763).
Please note: ab214342 only recognises the Ca2+ activated form of Human Gelsolin.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituents: PBS, 2.9% Sodium chloride
Concentration information loading...
PurityPurified via His tag
Our Abpromise guarantee covers the use of ab214342 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).|
|IP||Use at an assay dependent concentration.|
FunctionCalcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
Tissue specificityPhagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
Involvement in diseaseDefects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.
Sequence similaritiesBelongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats.
modificationsPhosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
Cellular localizationCytoplasm > cytoskeleton and Secreted.
- Information by UniProt
- Actin depolymerizing factor antibody
- Actin-depolymerizing factor antibody
- ADF antibody
Anti-Gelsolin antibody (ab214342) was conjugated to NHS activated magnetic beads at 1 mg of antibody per 1 ml of slurry.
20 ul of resulting slurry was combined with 200 ug of protein extracted from HeLa cells using RIPA buffer.
Lane 1: 10% input
Lane 2: Immunoprecipitated material
All lanes : Anti-Gelsolin antibody (ab214342) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Human kidney tissue lysate - total protein
Lane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Mouse monoclonal [M2] to DDDDK tag - (Equivalent to FLAG antibodies from Sigma) (HRP) (ab49763) at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 86 kDa
Observed band size: 86 kDa
Exposure time: 10 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with abX overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
ab214342 has not yet been referenced specifically in any publications.