Overview

  • Product name

  • Description

    Alpaca monoclonal to Gelsolin
  • Host species

    Alpaca
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Gelsolin.
    Database link: P06396

  • General notes

    Contains a FLAG tag (DDDDK) and a His tag Therefore, an anti DDDDK or anti His secondary can be used. We recommend the anti-FLAG option (ab49763).

    Please note: ab214342 only recognises the Ca2+ activated form of Human Gelsolin.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab214342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
IP Use at an assay dependent concentration.

Target

  • Function

    Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
  • Tissue specificity

    Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
  • Involvement in disease

    Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.
  • Sequence similarities

    Belongs to the villin/gelsolin family.
    Contains 6 gelsolin-like repeats.
  • Post-translational
    modifications

    Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
  • Cellular localization

    Cytoplasm > cytoskeleton and Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Actin depolymerizing factor antibody
    • Actin-depolymerizing factor antibody
    • ADF antibody
    • AGEL antibody
    • Brevin antibody
    • DKFZp313L0718 antibody
    • GELS_HUMAN antibody
    • Gelsolin antibody
    • Gsn antibody
    see all

Images

  • Anti-Gelsolin antibody (ab214342) was conjugated to NHS activated magnetic beads at 1 mg of antibody per 1 ml of slurry.

    20 ul of resulting slurry was combined with 200 ug of protein extracted from HeLa cells using RIPA buffer.

    Samples were analysed by western blot using anti Gelsolin primary antibody (ab74420 at 1 ug/ml) and anti Rabbit secondary antibody (ab201489 at 1/50000).

    Lane 1: 10% input

    Lane 2: Immunoprecipitated material

  • All lanes : Anti-Gelsolin antibody (ab214342) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Human kidney tissue lysate - total protein
    Lane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Mouse monoclonal [M2] to DDDDK tag - (Equivalent to FLAG antibodies from Sigma) (HRP) (ab49763) at 1/1000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 86 kDa
    Observed band size: 86 kDa


    Exposure time: 10 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with abX overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

References

ab214342 has not yet been referenced specifically in any publications.

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