Overview

  • Product name
  • Description
    Goat polyclonal to Gelsolin
  • Host species
    Goat
  • Specificity
    This antibody is expected to recognize both reported isoforms (NP_000168.1 and NP_937895.1).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Gelsolin aa 655-666 (internal sequence).
    Sequence:

    C-PRLKDKKMDAHP

  • Positive control
    • Human Spleen lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 0.5% Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 86 kDa).

Target

  • Function
    Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
  • Tissue specificity
    Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
  • Involvement in disease
    Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.
  • Sequence similarities
    Belongs to the villin/gelsolin family.
    Contains 6 gelsolin-like repeats.
  • Post-translational
    modifications
    Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
  • Cellular localization
    Cytoplasm > cytoskeleton and Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin depolymerizing factor antibody
    • Actin-depolymerizing factor antibody
    • ADF antibody
    • AGEL antibody
    • Brevin antibody
    • DKFZp313L0718 antibody
    • GELS_HUMAN antibody
    • Gelsolin antibody
    • Gsn antibody
    see all

Images

  • Anti-Gelsolin antibody (ab53560) at 0.1 µg/ml + Human Spleen lysate (35µg protein in RIPA buffer).

    Predicted band size: 86 kDa
    Observed band size: 90 kDa (why is the actual band size different from the predicted?)



    Primary incubation was 1 hour. Detected by chemiluminescence.

References

ab53560 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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