Product nameAnti-Gelsolin antibody
See all Gelsolin primary antibodies
DescriptionRabbit polyclonal to Gelsolin
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Gelsolin aa 750 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available as
- Recombinant Human Gelsolin plasma protein (ab114279) can be used as a positive control in WB. This antibody gave a positive signal in the following tissue lysates: Rat Kidney, Mouse Spleen, Rat Thymus
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab74420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).Can be blocked with Human Gelsolin peptide (ab74419).|
FunctionCalcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
Tissue specificityPhagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
Involvement in diseaseDefects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.
Sequence similaritiesBelongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats.
modificationsPhosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
Cellular localizationCytoplasm > cytoskeleton and Secreted.
- Information by UniProt
- Actin depolymerizing factor antibody
- Actin-depolymerizing factor antibody
- ADF antibody
All lanes : Anti-Gelsolin antibody (ab74420) at 1 µg/ml
Lane 1 : Kidney (Rat) Tissue Lysate
Lane 2 : Spleen (Mouse) Tissue Lysate
Lane 3 : Thymus (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 86 kDa
Observed band size: 86 kDa
Additional bands at: 120 kDa, 140 kDa, 52 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
ICC/IF image of ab74420 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74420, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) PC12 cells at 1µg/ml.
ab74420 staining Gelsolin in human brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
The black arrow indicates a gelsolin-positive Lewy body.
This product has been referenced in:
- Jalali BM et al. Alterations in the distribution of actin and its binding proteins in the porcine endometrium during early pregnancy: Possible role in epithelial remodeling and embryo adhesion. Theriogenology 116:17-27 (2018). Read more (PubMed: 29763784) »
- Putinski C et al. Caspase Cleavage of Gelsolin Is an Inductive Cue for Pathologic Cardiac Hypertrophy. J Am Heart Assoc 7:e010404 (2018). Read more (PubMed: 30486716) »