Overview

  • Product name

    Anti-Gelsolin antibody - C-terminal
    See all Gelsolin primary antibodies
  • Description

    Rabbit polyclonal to Gelsolin - C-terminal
  • Host species

    Rabbit
  • Specificity

    No cross reactivity with other proteins.
  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Monkey
    Predicted to work with: Horse, Chicken, Cow, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Gelsolin aa 763-775 (C terminal).
    Sequence:

    WDDDYWSVDPLDR


    Database link: P06396

  • Positive control

    • NIH3T3 cells; Rat cardiac muscle tissue; Human lung cancer tissue; Human intestinal cancer tissue; HeLa and A431 cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 85 kDa.

The detection limit for Gelsolin is approximately 1.5ng/lane under reducing conditions.

IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
  • Tissue specificity

    Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
  • Involvement in disease

    Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.
  • Sequence similarities

    Belongs to the villin/gelsolin family.
    Contains 6 gelsolin-like repeats.
  • Post-translational
    modifications

    Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
  • Cellular localization

    Cytoplasm > cytoskeleton and Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Actin depolymerizing factor antibody
    • Actin-depolymerizing factor antibody
    • ADF antibody
    • AGEL antibody
    • Brevin antibody
    • DKFZp313L0718 antibody
    • GELS_HUMAN antibody
    • Gelsolin antibody
    • Gsn antibody
    see all

Images

  • All lanes : Anti-Gelsolin antibody - C-terminal (ab191134) at 0.5 µg/ml

    Lane 1 : human HeLa whole cell lysate
    Lane 2 : human THP-1 whole cell lysate
    Lane 3 : human A549 whole cell lysate
    Lane 4 : monkey COS-7 whole cell lysate
    Lane 5 : human Caco-2 whole cell lysate
    Lane 6 : human K562 whole cell lysate
    Lane 7 : human HL-60 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG-HRP at 1/10000 dilution

    Predicted band size: 85 kDa

  • Immunohistochemical analysis of paraffin-embedded Human intestinal cancer tissue labeling Gelsolin with ab191134 at 1 μg/ml.

  • Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Gelsolin with ab191134 at 1 μg/ml.

  • All lanes : Anti-Gelsolin antibody - C-terminal (ab191134) at 0.5 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate

    Predicted band size: 85 kDa

  • Immunocytochemical analysis of NIH3T3 cells labeling Gelsolin with ab191134 at 1 μg/ml.

  • Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Gelsolin with ab191134 at 1 μg/ml.

References

ab191134 has not yet been referenced specifically in any publications.

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