RabMAb

Anti-Gelsolin antibody [EP1940Y] (ab134183)

Submit a review Q&A (1)References (3)

Overview

  • Product name

    Anti-Gelsolin antibody [EP1940Y]
    See all Gelsolin primary antibodies

  • Description

    Rabbit monoclonal [EP1940Y] to Gelsolin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Human Gelsolin aa 650-750 (C terminal).
    Database link: P06396

  • Positive control

    • NIH3T3 cell lysate; Human tonsil tissue

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EP1940Y

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 86 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.

    • Target

    • Function

      Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.

    • Tissue specificity

      Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.

    • Involvement in disease

      Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.

    • Sequence similarities

      Belongs to the villin/gelsolin family.
      Contains 6 gelsolin-like repeats.

    • Post-translational
      modifications

      Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.

    • Cellular localization

      Cytoplasm > cytoskeleton and Secreted.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Actin depolymerizing factor antibody
      • Actin-depolymerizing factor antibody
      • ADF antibody
      • AGEL antibody
      • Brevin antibody
      • DKFZp313L0718 antibody
      • GELS_HUMAN antibody
      • Gelsolin antibody
      • Gsn antibody
      see all

    Images

    • Western blot - Anti-Gelsolin antibody [EP1940Y] (ab134183)
      Western blot - Anti-Gelsolin antibody [EP1940Y] (ab134183)
      All lanes : Anti-Gelsolin antibody [EP1940Y] (ab134183) at 1/10000 dilution

      Lane 1 : THP-1 whole cell lysate
      Lane 2 : THP-1 cultured medium
      Lane 3 : MCF-7 whole cell lysate
      Lane 4 : Human plasma

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 86 kDa
      Observed band size: 81/86 kDa
      why is the actual band size different from the predicted?


      Exposure time: 10 seconds


      Blockiing buffer 5% NFDM/TBST
      Diluting buffer 5% NFDM/TBST

      The secretory isoform of gelsolin is at 86kDa. The cytoplasmic isoforms of gelsolin are at 81kDa.

    • Western blot - Anti-Gelsolin antibody [EP1940Y] (ab134183)
      Western blot - Anti-Gelsolin antibody [EP1940Y] (ab134183)
      Anti-Gelsolin antibody [EP1940Y] (ab134183) at 1/5000 dilution + NIH3T3 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 86 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody [EP1940Y] (ab134183)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody [EP1940Y] (ab134183)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling Gelsolin with ab134183 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Dai B  et al. MiR-21 protected against diabetic cardiomyopathy induced diastolic dysfunction by targeting gelsolin. Cardiovasc Diabetol 17:123 (2018). Read more (PubMed: 30180843) »
    • Shah AK  et al. Serum Glycoprotein Biomarker Discovery and Qualification Pipeline Reveals Novel Diagnostic Biomarker Candidates for Esophageal Adenocarcinoma. Mol Cell Proteomics 14:3023-39 (2015). Read more (PubMed: 26404905) »
    See all 3 Publications for this product

    Publishing research using ab134183? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    Dear Abcam,

    Thank you for your consideration in getting back to me in a timely manner about ab82267. The reference number for that order was ***.Would it be possible to have the antibody replaced with ab134183?

    Thanks,

    Read More
    Answer

    Thank you very much for your reply.

    I'm sending a free of charge vial of ab134183 on the order ***. This vial is transferring from our office in the UK, so we expect it to arrive in about a week. I apologize for the delay in getting this replacement to you.

    Please keep me updated about the results using this replacement antibody, and if you have any questions or need anything else, please let me know and I'll be happy to help.

    Read More

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