Product nameAnti-Gelsolin antibody [EP1940Y]
See all Gelsolin primary antibodies
DescriptionRabbit monoclonal [EP1940Y] to Gelsolin
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human Gelsolin aa 650-750 (C terminal).
Database link: P06396
- NIH3T3 cell lysate; Human tonsil tissue
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 86 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionCalcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
Tissue specificityPhagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
Involvement in diseaseDefects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.
Sequence similaritiesBelongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats.
modificationsPhosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
Cellular localizationCytoplasm > cytoskeleton and Secreted.
- Information by UniProt
- Actin depolymerizing factor antibody
- Actin-depolymerizing factor antibody
- ADF antibody
All lanes : Anti-Gelsolin antibody [EP1940Y] (ab134183) at 1/10000 dilution
Lane 1 : THP-1 whole cell lysate
Lane 2 : THP-1 cultured medium
Lane 3 : MCF-7 whole cell lysate
Lane 4 : Human plasma
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 86 kDa
Observed band size: 81/86 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
Blockiing buffer 5% NFDM/TBST
Diluting buffer 5% NFDM/TBST
The secretory isoform of gelsolin is at 86kDa. The cytoplasmic isoforms of gelsolin are at 81kDa.
Anti-Gelsolin antibody [EP1940Y] (ab134183) at 1/5000 dilution + NIH3T3 cell lysate at 10 µg
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 86 kDa
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling Gelsolin with ab134183 at 1/100 dilution.
This product has been referenced in:
- Dai B et al. MiR-21 protected against diabetic cardiomyopathy induced diastolic dysfunction by targeting gelsolin. Cardiovasc Diabetol 17:123 (2018). Read more (PubMed: 30180843) »
- Shah AK et al. Serum Glycoprotein Biomarker Discovery and Qualification Pipeline Reveals Novel Diagnostic Biomarker Candidates for Esophageal Adenocarcinoma. Mol Cell Proteomics 14:3023-39 (2015). Read more (PubMed: 26404905) »