Anti-Gelsolin antibody [EP1940Y] (ab134183)
Key features and details
- Rabbit monoclonal [EP1940Y] to Gelsolin
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-Gelsolin antibody [EP1940Y]
See all Gelsolin primary antibodies -
Description
Rabbit monoclonal [EP1940Y] to Gelsolin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human Gelsolin aa 650-750 (C terminal).
Database link: P06396 -
Positive control
- NIH3T3 cell lysate; Human tonsil tissue
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1940Y -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 86 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 86 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis. -
Tissue specificity
Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells. -
Involvement in disease
Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure. -
Sequence similarities
Belongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats. -
Post-translational
modificationsPhosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids. -
Cellular localization
Cytoplasm > cytoskeleton and Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2934 Human
- Entrez Gene: 227753 Mouse
- Entrez Gene: 296654 Rat
- Omim: 137350 Human
- SwissProt: P06396 Human
- SwissProt: P13020 Mouse
- SwissProt: Q68FP1 Rat
- Unigene: 522373 Human
see all -
Alternative names
- Actin depolymerizing factor antibody
- Actin-depolymerizing factor antibody
- ADF antibody
see all
Images
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All lanes : Anti-Gelsolin antibody [EP1940Y] (ab134183) at 1/10000 dilution
Lane 1 : THP-1 whole cell lysate
Lane 2 : THP-1 cultured medium
Lane 3 : MCF-7 whole cell lysate
Lane 4 : Human plasma
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 86 kDa
Observed band size: 81,86 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlockiing buffer 5% NFDM/TBST
Diluting buffer 5% NFDM/TBSTThe secretory isoform of gelsolin is at 86kDa. The cytoplasmic isoforms of gelsolin are at 81kDa.
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Anti-Gelsolin antibody [EP1940Y] (ab134183) at 1/5000 dilution + NIH3T3 cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 86 kDa -
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling Gelsolin with ab134183 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab134183 has been referenced in 3 publications.
- Dai B et al. MiR-21 protected against diabetic cardiomyopathy induced diastolic dysfunction by targeting gelsolin. Cardiovasc Diabetol 17:123 (2018). PubMed: 30180843
- Shah AK et al. Serum Glycoprotein Biomarker Discovery and Qualification Pipeline Reveals Novel Diagnostic Biomarker Candidates for Esophageal Adenocarcinoma. Mol Cell Proteomics 14:3023-39 (2015). PubMed: 26404905
- Skibinski CG et al. Proteomic changes induced by effective chemopreventive ratios of n-3:n-6 fatty acids and tamoxifen against MNU-induced mammary cancer in the rat. Cancer Prev Res (Phila) 6:979-88 (2013). PubMed: 23880232