Product nameAnti-Gelsolin antibody [GS-2C4]
See all Gelsolin primary antibodies
DescriptionMouse monoclonal [GS-2C4] to Gelsolin
Tested applicationsSuitable for: Other, ELISA, WB, ICC/IF, IHC-Frmore details
Species reactivityReacts with: Sheep, Rabbit, Cow, Human, Pig
Full length native protein (purified) corresponding to Human Gelsolin.
Database link: P06396-1
EpitopeThe antibody is directed against an epitope located on the 47 kD peptide derived from a chymotryptic cleavage of gelsolin. This peptide contains the carboxy terminal actin binding site for gelsolin.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.097% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab11081 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Other||Use at an assay dependent concentration.|
|AP||Use at an assay dependent concentration.|
|WB||1/1000. Predicted molecular weight: 86 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionCalcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
Tissue specificityPhagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
Involvement in diseaseDefects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.
Sequence similaritiesBelongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats.
modificationsPhosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
Cellular localizationCytoplasm > cytoskeleton and Secreted.
- Information by UniProt
- Actin depolymerizing factor antibody
- Actin-depolymerizing factor antibody
- ADF antibody
Anti-Gelsolin antibody [GS-2C4] (ab11081) at 1/2000 dilution + Human Plasma
AP conjugated anti-mouse Fab IgG
Performed under reducing conditions.
Predicted band size: 86 kDa
ICC/IF image of ab11081 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11081, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.
This product has been referenced in:
- Huang BP et al. Upregulation of heat shock protein 70 and the differential protein expression induced by tumor necrosis factor-alpha enhances migration and inhibits apoptosis of hepatocellular carcinoma cell HepG2. Int J Med Sci 14:284-293 (2017). WB . Read more (PubMed: 28367089) »
- Soleilhavoup C et al. Proteomes of the Female Genital Tract During the Oestrous Cycle. Mol Cell Proteomics 15:93-108 (2016). Read more (PubMed: 26518761) »