Overview

  • Product name
    Anti-Gelsolin antibody [GS-2C4]
    See all Gelsolin primary antibodies
  • Description
    Mouse monoclonal [GS-2C4] to Gelsolin
  • Host species
    Mouse
  • Tested applications
    Suitable for: Other, ELISA, WB, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Sheep, Rabbit, Cow, Human, Pig
  • Immunogen

    Full length native protein (purified) corresponding to Human Gelsolin.
    Database link: P06396-1

  • Epitope
    The antibody is directed against an epitope located on the 47 kD peptide derived from a chymotryptic cleavage of gelsolin. This peptide contains the carboxy terminal actin binding site for gelsolin.

Properties

Applications

Our Abpromise guarantee covers the use of ab11081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Other Use at an assay dependent concentration.
ELISA 1/1000.
AP Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 86 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
  • Tissue specificity
    Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
  • Involvement in disease
    Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.
  • Sequence similarities
    Belongs to the villin/gelsolin family.
    Contains 6 gelsolin-like repeats.
  • Post-translational
    modifications
    Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
  • Cellular localization
    Cytoplasm > cytoskeleton and Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin depolymerizing factor antibody
    • Actin-depolymerizing factor antibody
    • ADF antibody
    • AGEL antibody
    • Brevin antibody
    • DKFZp313L0718 antibody
    • GELS_HUMAN antibody
    • Gelsolin antibody
    • Gsn antibody
    see all

Images

  • Anti-Gelsolin antibody [GS-2C4] (ab11081) at 1/2000 dilution + Human Plasma

    Secondary
    AP conjugated anti-mouse Fab IgG

    Performed under reducing conditions.

    Predicted band size: 86 kDa

  • ICC/IF image of ab11081 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11081, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.

References

This product has been referenced in:
  • Huang BP  et al. Upregulation of heat shock protein 70 and the differential protein expression induced by tumor necrosis factor-alpha enhances migration and inhibits apoptosis of hepatocellular carcinoma cell HepG2. Int J Med Sci 14:284-293 (2017). WB . Read more (PubMed: 28367089) »
  • Soleilhavoup C  et al. Proteomes of the Female Genital Tract During the Oestrous Cycle. Mol Cell Proteomics 15:93-108 (2016). Read more (PubMed: 26518761) »
See all 17 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (8-16%)
Sample
Sheep Serum (Cervical mucus)
Specification
Cervical mucus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Mr. Guillaume Tsikis

Verified customer

Submitted Jul 05 2013

Answer

Thank you for your inquiry and please excuse the delayed answer. I was in contact with the source of this antibody and did a literature search, but I could not find any other anti-gelsolin antibodies that were used with ab11081 as part of a sandwich ELISA. We have two other polyclonal antibodies against gelsolin that are tested in ELISA so far. ab22545: Click here (or use the following: https://www.abcam.com/index.html?datasheet=22545). ab35290: Click here (or use the following: https://www.abcam.com/index.html?datasheet=35290). If you would like to test one with ab11081 in a sandwich ELISA, please let me know because I would like to offer you to take part in our testing discount program. I hope this information is nevertheless helpful. I am looking forward to your reply.

Read More

Answer

Thank you for contacting us. The immunogen is the full-length human gelsolin, and the epitope that it binds to is located somewhere on the carboxyl-terminal two-thirds. The following reference describes production of monoclonal antibodies against human plasma gelsolin, including this clone, 2C4. The actin filament-severing domain of plasma gelsolin. Chaponnier C, Janmey PA, Yin HL. J Cell Biol. 1986 Oct;103(4):1473-81. PMID: 3021782 From page 1476: "This (47 kD) peptide, and a 45-kD peptide with similar actin-binding properties reacted with the monoclonal antibody designated 2C4 (Fig. 2 c). These two peptides are therefore related and contain the same actin-binding domain which is shown in Kwiatkowski et al. (1985) to be derived from the carboxyl-terminal two-thirds of plasma gelsolin." I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (glioblastoma cell lines)
Loading amount
8 µg
Specification
glioblastoma cell lines
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Nov 22 2006

Answer

The products, as long as you follow the storage instructions can be kept up to 5years,you should aliquot them and store them at -20°C and avoid freeze / thaw cycles. If you need any further information, please don't hestitate to contact us.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Serum (Serum)
Specification
Serum
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Type
Sandwich

Abcam user community

Verified customer

Submitted Aug 08 2006

Answer

Thank you for your enquiry. Ab11081 reacts specifically with human plasma and cytoplasmic gelsolin in immunoblotting. The antibody is directed against an epitope located on the 47 kD peptide derived from a chymotryptic cleavage of gelsolin. This peptide contains the carboxy terminal actin binding site for gelsolin. Gelsolin has been shown to exist in at least two variant forms, cytoplasmic gelsolin (from rabbit macrophages and human platelets) and plasma gelsolin (from human or bovine plasma). Human plasma and rabbit macrophage gelsolins differ by the presence of a 25 amino acid residue extension at the NH2 terminus in the human plasma gelsolin which appears to account for the difference in relative molecular weights. I think this should work on human platelets but you may have to work on the titration for using it. We have seen two doublet bands at 97 kDa and it's possible that you will see a lower band under reduced conditions. For ab2500, you should see a band at approximately 210 kD. Please contact us again if you have any additional questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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