Anti-Gelsolin plasma antibody (ab136832)

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Overview

  • Product name

    Anti-Gelsolin plasma antibody
    See all Gelsolin plasma primary antibodies

  • Description

    Goat polyclonal to Gelsolin plasma

  • Host species

    Goat

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to Human Gelsolin plasma aa 40-51 (Cysteine residue).
    Sequence:

    PQGRVPEARPNS-C

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human colon, duodenum heart and skeletal muscle lysates.

  • General notes

    Reported variants represent identical protein: NP_001121138.1, NP_001121139.1 Reported variants represent identical protein: NP_001121135.1, NP_937895.1, NP_001121134.1, NP_001121137.1, NP_001121136.1.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    ab136832 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab136832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 86 kDa.

Target

  • Relevance

    Gelsolin is a calcium regulated, actin modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. It plays a role in phosphoinositide mediated disassembly of actin filaments in Sertoli cell adhesion complexes; it may regulate sperm release and turnover of the blood testis barrier [RGD], and severs actin filaments in the presence of submicromolar calcium, thereby solating cytoplasmic actin gels. A calcium independent mechanism reverses the process. Northern blot analysis suggested that a single gene encodes both cell and plasma gelsolins. This protein may be unique in that it is made for both secretion and intracytoplasmic location.

  • Cellular localization

    Secreted

  • Database links

    • Alternative names

      • Actin depolymerizing factor antibody
      • ADF antibody
      • AGEL antibody
      • Brevin antibody
      • Gelsolin antibody
      see all

    Images

    • Western blot - Anti-Gelsolin plasma antibody (ab136832)
      Western blot - Anti-Gelsolin plasma antibody (ab136832)
      Anti-Gelsolin plasma antibody (ab136832) at 1 µg/ml + Human duodenum lysate (in RIPA buffer) at 35 µg

      Secondary
      HRP labelled rabbit anti-goat at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 86 kDa

    References

    ab136832 has not yet been referenced specifically in any publications.

    Publishing research using ab136832? Please let us know so that we can cite the reference in this datasheet.

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