Anti-Gelsolin plasma antibody (ab136832)
Key features and details
- Goat polyclonal to Gelsolin plasma
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-Gelsolin plasma antibody
See all Gelsolin plasma primary antibodies -
Description
Goat polyclonal to Gelsolin plasma -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Gelsolin plasma aa 40-51 (Cysteine residue).
Sequence:PQGRVPEARPNS-C
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Positive control
- Human colon, duodenum heart and skeletal muscle lysates.
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General notes
Reported variants represent identical protein: NP_001121138.1, NP_001121139.1 Reported variants represent identical protein: NP_001121135.1, NP_937895.1, NP_001121134.1, NP_001121137.1, NP_001121136.1.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab136832 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab136832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 86 kDa. 1 hour primary incubation is recommended for this product. |
Target
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Relevance
Gelsolin is a calcium regulated, actin modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. It plays a role in phosphoinositide mediated disassembly of actin filaments in Sertoli cell adhesion complexes; it may regulate sperm release and turnover of the blood testis barrier [RGD], and severs actin filaments in the presence of submicromolar calcium, thereby solating cytoplasmic actin gels. A calcium independent mechanism reverses the process. Northern blot analysis suggested that a single gene encodes both cell and plasma gelsolins. This protein may be unique in that it is made for both secretion and intracytoplasmic location. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 2934 Human
- Omim: 137350 Human
- SwissProt: P06396 Human
- Unigene: 522373 Human
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Alternative names
- Actin depolymerizing factor antibody
- ADF antibody
- AGEL antibody
see all
Images
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Western blot - Anti-Gelsolin plasma antibody (ab136832)Anti-Gelsolin plasma antibody (ab136832) at 1 µg/ml + Human duodenum lysate (in RIPA buffer) at 35 µg
Secondary
HRP labelled rabbit anti-goat at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 86 kDa
Datasheets and documents
References (0)
ab136832 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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