Immunocytochemistry/ Immunofluorescence abreview for Anti-GEMC1 antibody

Inconclusive
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Fibroblasts)
Specification
Fibroblasts
Fixative
Paraformaldehyde
Permeabilization
Yes - triton X-100.
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3%BSA/10%FBS

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor® 488 Goat Anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Additional data

Additional Notes
The antibody didn't work after methanol fixation.
Also, this antibody didn't work in dilution 1/1000 or 1/2000

DISCOUNT CODE:ABTRIAL-EBSWV
Abcam response
Thanks for your honest feedback and for testing ab112571 in ICC/IF.

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Submitted Jan 16 2013

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