For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Mouse Cell (Fibroblasts)
Yes - triton X-100.
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3%BSA/10%FBS
Non-Abcam antibody was used: Alexa Fluor® 488 Goat Anti-Rabbit IgG (H+L)
Host species: Goat
Conjugation: Alexa Fluor® 488
The antibody didn't work after methanol fixation.
Also, this antibody didn't work in dilution 1/1000 or 1/2000
Thanks for your honest feedback and for testing ab112571 in ICC/IF.
Abcam user community
Submitted Jan 16 2013