Anti-Gemin 2 antibody [2E17] (ab6084)
![Flow Cytometry - Anti-Gemin 2 antibody [2E17] (ab6084)](https://www.abcam.com/ps/products/6/ab6084/Images/ab6084-12015-anti-gemin-2-antibody-2e17-flow-cytometry.jpg "Flow Cytometry - Anti-Gemin 2 antibody [2E17] (ab6084)")
Key features and details
- Mouse monoclonal [2E17] to Gemin 2
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-Gemin 2 antibody [2E17]
See all Gemin 2 primary antibodies -
Description
Mouse monoclonal [2E17] to Gemin 2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
-
Immunogen
His-Gemin 2 fusion protein (amino acids 12 - 280, starting at 2nd methionine).
-
General notes
Involved in biogenesis of spliceosomal snRNPs, forms complex with Survival of Motor Neurons (SMN) protein, interacts tightly with spliceosomal proteins and spliceosomal snRNAs U1 and U5.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from supernatant. -
Primary antibody notes
Involved in biogenesis of spliceosomal snRNPs, forms complex with Survival of Motor Neurons (SMN) protein, interacts tightly with spliceosomal proteins and spliceosomal snRNAs U1 and U5. -
Clonality
Monoclonal -
Clone number
2E17 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
-
Function
The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. -
Sequence similarities
Belongs to the gemin-2 family. -
Cellular localization
Nucleus > gem. Cytoplasm. Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs. Also found in the cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 8487 Human
- Omim: 602595 Human
- SwissProt: O14893 Human
- Unigene: 652307 Human
-
Alternative names
- Component of gems 2 antibody
- Gem (nuclear organelle) associated protein 2 antibody
- Gem associated protein 2 antibody
see all
Images
-
Flow Cytometry - Anti-Gemin 2 antibody [2E17] (ab6084)Overlay histogram showing HeLa cells stained with ab6084 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6084, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Datasheets and documents
-
SDS download
-
Datasheet download
References (8)
ab6084 has been referenced in 8 publications.
- Staropoli JF et al. Rescue of gene-expression changes in an induced mouse model of spinal muscular atrophy by an antisense oligonucleotide that promotes inclusion of SMN2 exon 7. Genomics 105:220-8 (2015). PubMed: 25645699
- Groen EJ et al. ALS-associated mutations in FUS disrupt the axonal distribution and function of SMN. Hum Mol Genet N/A:N/A (2013). PubMed: 23681068
- Sanchez G et al. A novel function for the survival motoneuron protein as a translational regulator. Hum Mol Genet : (2012). PubMed: 23136128
- Wu CY et al. Proteomic assessment of a cell model of spinal muscular atrophy. BMC Neurosci 12:25 (2011). WB ; Mouse . PubMed: 21385431
- Burnett BG et al. Regulation of SMN protein stability. Mol Cell Biol 29:1107-15 (2009). WB ; Human . PubMed: 19103745
- Walker MP et al. SMN complex localizes to the sarcomeric Z-disc and is a proteolytic target of calpain. Hum Mol Genet 17:3399-410 (2008). ICC/IF ; Mouse . PubMed: 18689355
- Morency E et al. A novel cell response triggered by interphase centromere structural instability. J Cell Biol 177:757-68 (2007). ICC/IF ; Human . PubMed: 17548509
- Renvoisé B et al. Distinct domains of the spinal muscular atrophy protein SMN are required for targeting to Cajal bodies in mammalian cells. J Cell Sci 119:680-92 (2006). PubMed: 16449324