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Anti-Gemin 2 antibody [2E17] (ab6084)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (8)

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Flow Cytometry - Anti-Gemin 2 antibody [2E17] (ab6084)

    Key features and details

    • Mouse monoclonal [2E17] to Gemin 2
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG1

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    View more associated products

    Overview

    • Product name

      Anti-Gemin 2 antibody [2E17]
      See all Gemin 2 primary antibodies
    • Description

      Mouse monoclonal [2E17] to Gemin 2
    • Host species

      Mouse
    • Tested Applications & Species

      Application Species
      Flow Cyt
      Human
      See all applications and species data
    • Immunogen

      His-Gemin 2 fusion protein (amino acids 12 - 280, starting at 2nd methionine).

    • General notes


      Involved in biogenesis of spliceosomal snRNPs, forms complex with Survival of Motor Neurons (SMN) protein, interacts tightly with spliceosomal proteins and spliceosomal snRNAs U1 and U5.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Purification notes

      Purified from supernatant.
    • Primary antibody notes

      Involved in biogenesis of spliceosomal snRNPs, forms complex with Survival of Motor Neurons (SMN) protein, interacts tightly with spliceosomal proteins and spliceosomal snRNAs U1 and U5.
    • Clonality

      Monoclonal
    • Clone number

      2E17
    • Myeloma

      Sp2/0
    • Isotype

      IgG1
    • Research areas

      • Neuroscience
      • Neurology process
      • Neural Signal Transduction

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
      • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
      • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6084 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    Flow Cyt
    Human
    Application Abreviews Notes
    Flow Cyt
    Use 1µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Notes
    Flow Cyt
    Use 1µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus.
    • Sequence similarities

      Belongs to the gemin-2 family.
    • Cellular localization

      Nucleus > gem. Cytoplasm. Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs. Also found in the cytoplasm.
    • Target information above from: UniProt accession O14893 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 8487 Human
      • Omim: 602595 Human
      • SwissProt: O14893 Human
      • Unigene: 652307 Human
      • Alternative names

        • Component of gems 2 antibody
        • Gem (nuclear organelle) associated protein 2 antibody
        • Gem associated protein 2 antibody
        • GEMI2_HUMAN antibody
        • Gemin-2 antibody
        • gemin2 antibody
        • SIP 1 antibody
        • SIP-1 antibody
        • SIP1 antibody
        • SIP1 delta antibody
        • SIP1-delta antibody
        • SMN interacting protein 1 antibody
        • SMN interacting protein 1 delta antibody
        • SMN-interacting protein 1 antibody
        • Survival interacting protein 1 antibody
        • Survival of motor neuron protein interacting protein 1 antibody
        • Survival of motor neuron protein-interacting protein 1 antibody
        see all

      Images

      • Flow Cytometry - Anti-Gemin 2 antibody [2E17] (ab6084)
        Flow Cytometry - Anti-Gemin 2 antibody [2E17] (ab6084)
        Overlay histogram showing HeLa cells stained with ab6084 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6084, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (8)

      Publishing research using ab6084? Please let us know so that we can cite the reference in this datasheet.

      ab6084 has been referenced in 8 publications.

      • Staropoli JF  et al. Rescue of gene-expression changes in an induced mouse model of spinal muscular atrophy by an antisense oligonucleotide that promotes inclusion of SMN2 exon 7. Genomics 105:220-8 (2015). PubMed: 25645699
      • Groen EJ  et al. ALS-associated mutations in FUS disrupt the axonal distribution and function of SMN. Hum Mol Genet N/A:N/A (2013). PubMed: 23681068
      • Sanchez G  et al. A novel function for the survival motoneuron protein as a translational regulator. Hum Mol Genet : (2012). PubMed: 23136128
      • Wu CY  et al. Proteomic assessment of a cell model of spinal muscular atrophy. BMC Neurosci 12:25 (2011). WB ; Mouse . PubMed: 21385431
      • Burnett BG  et al. Regulation of SMN protein stability. Mol Cell Biol 29:1107-15 (2009). WB ; Human . PubMed: 19103745
      • Walker MP  et al. SMN complex localizes to the sarcomeric Z-disc and is a proteolytic target of calpain. Hum Mol Genet 17:3399-410 (2008). ICC/IF ; Mouse . PubMed: 18689355
      • Morency E  et al. A novel cell response triggered by interphase centromere structural instability. J Cell Biol 177:757-68 (2007). ICC/IF ; Human . PubMed: 17548509
      • Renvoisé B  et al. Distinct domains of the spinal muscular atrophy protein SMN are required for targeting to Cajal bodies in mammalian cells. J Cell Sci 119:680-92 (2006). PubMed: 16449324

      Customer reviews and Q&As

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      Immunocytochemistry/ Immunofluorescence abreview for Anti-Gemin 2 antibody [2E17]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Hela cells)
      Specification
      Hela cells
      Fixative
      Methanol
      Permeabilization
      No
      Blocking step
      Fish scale gelatin in PBS as blocking agent for 20 minute(s) · Concentration: 0.2% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 24 2012

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