Anti-Gemin 2 antibody [2E17] (ab6084)
- Datasheet
- Specific References (8)
- Protocols
Overview
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Product nameAnti-Gemin 2 antibody [2E17]
See all Gemin 2 primary antibodies -
DescriptionMouse monoclonal [2E17] to Gemin 2
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Host speciesMouse
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Tested applicationsSuitable for: Flow Cyt, ICC/IF, ELISA, WB, IPmore details
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Species reactivityReacts with: Mouse, Human, Xenopus laevis
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Immunogen
His-Gemin 2 fusion protein (amino acids 12 - 280, starting at 2nd methionine).
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General notes
Involved in biogenesis of spliceosomal snRNPs, forms complex with Survival of Motor Neurons (SMN) protein, interacts tightly with spliceosomal proteins and spliceosomal snRNAs U1 and U5.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityProtein A purified
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Purification notesPurified from supernatant.
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Primary antibody notesInvolved in biogenesis of spliceosomal snRNPs, forms complex with Survival of Motor Neurons (SMN) protein, interacts tightly with spliceosomal proteins and spliceosomal snRNAs U1 and U5.
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ClonalityMonoclonal
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Clone number2E17
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MyelomaSp2/0
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab6084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| ICC/IF | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 34 kDa. | |
| IP | Use at an assay dependent concentration. |
Target
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FunctionThe SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus.
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Sequence similaritiesBelongs to the gemin-2 family.
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Cellular localizationNucleus > gem. Cytoplasm. Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs. Also found in the cytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 8487 Human
- Entrez Gene: 66603 Mouse
- Omim: 602595 Human
- SwissProt: O14893 Human
- SwissProt: Q9CQQ4 Mouse
- SwissProt: O42260 Xenopus laevis
- Unigene: 652307 Human
- Unigene: 35353 Mouse
see all -
Alternative names
- Component of gems 2 antibody
- Gem (nuclear organelle) associated protein 2 antibody
- Gem associated protein 2 antibody
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Images
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![Flow Cytometry - Anti-Gemin 2 antibody [2E17] (ab6084)](https://www.abcam.com/ps/products/6/ab6084/Images/Gemin-2-Primary-antibodies-ab6084-1.jpg)
Flow Cytometry - Anti-Gemin 2 antibody [2E17] (ab6084)Overlay histogram showing HeLa cells stained with ab6084 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6084, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. -
![Immunocytochemistry/ Immunofluorescence - Anti-Gemin 2 antibody [2E17] (ab6084)](https://www.abcam.com/ps/products/6/ab6084/Images/Gemin-2-Primary-antibodies-ab6084-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Gemin 2 antibody [2E17] (ab6084)Image from Morency E et al, J Cell Biol. 2007 Jun 4;177(5):757-68, Fig 4f. DOI 10.1083/jcb.200612107.HeLa cells were transfected with an ICP0-expressing plasmid before IF assays to detect the distribution of CB- and gem-associated proteins. ab6084 used at a 1/200 dilution to detect Gemin 2 (green). Bar 5 µm.
Protocols
References
This product has been referenced in:
- Staropoli JF et al. Rescue of gene-expression changes in an induced mouse model of spinal muscular atrophy by an antisense oligonucleotide that promotes inclusion of SMN2 exon 7. Genomics 105:220-8 (2015). Read more (PubMed: 25645699) »
- Groen EJ et al. ALS-associated mutations in FUS disrupt the axonal distribution and function of SMN. Hum Mol Genet N/A:N/A (2013). Read more (PubMed: 23681068) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"