Anti-Gemin 2 antibody [2E17] (ab6084)
![Flow Cytometry - Anti-Gemin 2 antibody [2E17] (ab6084)](https://www.abcam.com/ps/products/6/ab6084/Images/ab6084-12015-anti-gemin-2-antibody-2e17-flow-cytometry.jpg "Flow Cytometry - Anti-Gemin 2 antibody [2E17] (ab6084)")
Key features and details
- Mouse monoclonal [2E17] to Gemin 2
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Gemin 2 antibody [2E17]
See all Gemin 2 primary antibodies -
Description
Mouse monoclonal [2E17] to Gemin 2 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
His-Gemin 2 fusion protein (amino acids 12 - 280, starting at 2nd methionine).
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General notes
Involved in biogenesis of spliceosomal snRNPs, forms complex with Survival of Motor Neurons (SMN) protein, interacts tightly with spliceosomal proteins and spliceosomal snRNAs U1 and U5.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from supernatant. -
Primary antibody notes
Involved in biogenesis of spliceosomal snRNPs, forms complex with Survival of Motor Neurons (SMN) protein, interacts tightly with spliceosomal proteins and spliceosomal snRNAs U1 and U5. -
Clonality
Monoclonal -
Clone number
2E17 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Function
The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. -
Sequence similarities
Belongs to the gemin-2 family. -
Cellular localization
Nucleus > gem. Cytoplasm. Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs. Also found in the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 8487 Human
- Omim: 602595 Human
- SwissProt: O14893 Human
- Unigene: 652307 Human
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Alternative names
- Component of gems 2 antibody
- Gem (nuclear organelle) associated protein 2 antibody
- Gem associated protein 2 antibody
see all
Images
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Flow Cytometry - Anti-Gemin 2 antibody [2E17] (ab6084)Overlay histogram showing HeLa cells stained with ab6084 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6084, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab6084 has been referenced in 10 publications.
- Kour S et al. Loss of function mutations in GEMIN5 cause a neurodevelopmental disorder. Nat Commun 12:2558 (2021). PubMed: 33963192
- Sapaly D et al. The Small-Molecule Flunarizine in Spinal Muscular Atrophy Patient Fibroblasts Impacts on the Gemin Components of the SMN Complex and TDP43, an RNA-Binding Protein Relevant to Motor Neuron Diseases. Front Mol Biosci 7:55 (2020). PubMed: 32363199
- Staropoli JF et al. Rescue of gene-expression changes in an induced mouse model of spinal muscular atrophy by an antisense oligonucleotide that promotes inclusion of SMN2 exon 7. Genomics 105:220-8 (2015). PubMed: 25645699
- Groen EJ et al. ALS-associated mutations in FUS disrupt the axonal distribution and function of SMN. Hum Mol Genet N/A:N/A (2013). PubMed: 23681068
- Sanchez G et al. A novel function for the survival motoneuron protein as a translational regulator. Hum Mol Genet : (2012). PubMed: 23136128
- Wu CY et al. Proteomic assessment of a cell model of spinal muscular atrophy. BMC Neurosci 12:25 (2011). WB ; Mouse . PubMed: 21385431
- Burnett BG et al. Regulation of SMN protein stability. Mol Cell Biol 29:1107-15 (2009). WB ; Human . PubMed: 19103745
- Walker MP et al. SMN complex localizes to the sarcomeric Z-disc and is a proteolytic target of calpain. Hum Mol Genet 17:3399-410 (2008). ICC/IF ; Mouse . PubMed: 18689355
- Morency E et al. A novel cell response triggered by interphase centromere structural instability. J Cell Biol 177:757-68 (2007). ICC/IF ; Human . PubMed: 17548509
- Renvoisé B et al. Distinct domains of the spinal muscular atrophy protein SMN are required for targeting to Cajal bodies in mammalian cells. J Cell Sci 119:680-92 (2006). PubMed: 16449324