Product nameAnti-Gemin 2 antibody
See all Gemin 2 primary antibodies
DescriptionRabbit polyclonal to Gemin 2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Sheep, Dog, Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla, Orangutan
- HeLa, 293T, Jurkat, TCMK-1 and NIH3T3 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab206690 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 186 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionThe SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus.
Sequence similaritiesBelongs to the gemin-2 family.
Cellular localizationNucleus > gem. Cytoplasm. Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs. Also found in the cytoplasm.
- Information by UniProt
- Component of gems 2 antibody
- Gem (nuclear organelle) associated protein 2 antibody
- Gem associated protein 2 antibody
All lanes : Anti-Gemin 2 antibody (ab206690) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate prepared using NETN lysis buffer
Lane 2 : 293T whole cell lysate prepared using NETN lysis buffer
Lane 3 : Jurkat whole cell lysate prepared using NETN lysis buffer
Lane 4 : Mouse TCMK-1 cells prepared using NETN lysis buffer
Lane 5 : Mouse NIH3T3 cells prepared using NETN lysis buffer
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 186 kDa
Exposure time: 30 seconds
4-8% SDS-PAGE gel
Detection of Gemin 2 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab206690 at 6 µg/mg lysate for IP and 0.4µg/ml for WB detection (Lane 1). Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
ab206690 has not yet been referenced specifically in any publications.