Overview

  • Product name

    Anti-Gemin 2 antibody [EPR10040(B)]
    See all Gemin 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR10040(B)] to Gemin 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Gemin 2 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • HepG2, K562 and HeLa cell lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10040(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function

      The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus.
    • Sequence similarities

      Belongs to the gemin-2 family.
    • Cellular localization

      Nucleus > gem. Cytoplasm. Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs. Also found in the cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • Component of gems 2 antibody
      • Gem (nuclear organelle) associated protein 2 antibody
      • Gem associated protein 2 antibody
      • GEMI2_HUMAN antibody
      • Gemin-2 antibody
      • gemin2 antibody
      • SIP 1 antibody
      • SIP-1 antibody
      • SIP1 antibody
      • SIP1 delta antibody
      • SIP1-delta antibody
      • SMN interacting protein 1 antibody
      • SMN interacting protein 1 delta antibody
      • SMN-interacting protein 1 antibody
      • Survival interacting protein 1 antibody
      • Survival of motor neuron protein interacting protein 1 antibody
      • Survival of motor neuron protein-interacting protein 1 antibody
      see all

    Images

    • All lanes : Anti-Gemin 2 antibody [EPR10040(B)] (ab150383) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 31 kDa

    References

    This product has been referenced in:

    • Rodriguez-Muela N  et al. Blocking p62-dependent SMN degradation ameliorates spinal muscular atrophy disease phenotypes. J Clin Invest 128:3008-3023 (2018). Read more (PubMed: 29672276) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab150383.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up