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    gemin-3-antibody-12h12-ab10305.pdf

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Anti-Gemin 3 antibody [12H12] (ab10305)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (6)

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Flow Cytometry - Anti-Gemin 3 antibody [12H12] (ab10305)

    Key features and details

    • Mouse monoclonal [12H12] to Gemin 3
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-Gemin 3 antibody [12H12]
      See all Gemin 3 primary antibodies
    • Description

      Mouse monoclonal [12H12] to Gemin 3
    • Host species

      Mouse
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Recombinant 6His-tag C-terminal domain of Gemin 3 (amino acids 368-548).

    • General notes


      The survival of motor neurons (SMN) gene is the disease gene of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The SMN protein is part of a complex containing several proteins, of which one, SIP1 (SMN interacting protein 1), has been characterized so far. The SMN complex is found in both the cytoplasm and in the nucleus, where it is concentrated in bodies called gems. In the cytoplasm, SMN and SIP1 interact with the Sm core proteins of spliceosomal small nuclear ribonucleoproteins (snRNPs), and they play a critical role in snRNP assembly. In the nucleus, SMN is required for pre-mRNA splicing, likely by serving in the regeneration of snRNPs. A DEAD box putative RNA helicase, named Gemin 3 which is another component of the SMN complex, has been identified. Gemin 3 interacts directly with SMN, as well as with SmB, SmD2 and SmD3. Immunolocalization studies using mAbs to Gemin 3 show that it colocalizes with SMN in gems. Gemin 3 binds SMN via its unique COOH-terminal domain, and SMN mutations found in some SMA patients strongly reduce this interaction. The presence of a DEAD box motif in Gemin 3 suggests that it may provide the catalytic activity that plays a critical role in the function of the SMN complex on RNPs.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Purification notes

      Protein A purified from tissue culture supernatant.
    • Primary antibody notes

      The survival of motor neurons (SMN) gene is the disease gene of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The SMN protein is part of a complex containing several proteins, of which one, SIP1 (SMN interacting protein 1), has been characterized so far. The SMN complex is found in both the cytoplasm and in the nucleus, where it is concentrated in bodies called gems. In the cytoplasm, SMN and SIP1 interact with the Sm core proteins of spliceosomal small nuclear ribonucleoproteins (snRNPs), and they play a critical role in snRNP assembly. In the nucleus, SMN is required for pre-mRNA splicing, likely by serving in the regeneration of snRNPs. A DEAD box putative RNA helicase, named Gemin 3 which is another component of the SMN complex, has been identified. Gemin 3 interacts directly with SMN, as well as with SmB, SmD2 and SmD3. Immunolocalization studies using mAbs to Gemin 3 show that it colocalizes with SMN in gems. Gemin 3 binds SMN via its unique COOH-terminal domain, and SMN mutations found in some SMA patients strongly reduce this interaction. The presence of a DEAD box motif in Gemin 3 suggests that it may provide the catalytic activity that plays a critical role in the function of the SMN complex on RNPs.
    • Clonality

      Monoclonal
    • Clone number

      12H12
    • Myeloma

      Sp2/0
    • Isotype

      IgG1
    • Research areas

      • Neuroscience
      • Neurology process
      • Neural Signal Transduction
      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • RNA Processing
      • Splicing

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
      • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
      • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab10305 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use 1µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Notes
    Flow Cyt
    Use 1µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. It may also play a role in the metabolism of snoRNPs.
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Belongs to the DEAD box helicase family. DDX20 subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Cellular localization

      Cytoplasm. Nucleus > gem. Localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies.
    • Target information above from: UniProt accession Q9UHI6 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 11218 Human
      • Omim: 606168 Human
      • SwissProt: Q9UHI6 Human
      • Unigene: 591405 Human
      • Alternative names

        • Component of gems 3 antibody
        • DDX 20 antibody
        • ddx20 antibody
        • DDX20_HUMAN antibody
        • DEAD (Asp Glu Ala Asp) box polypeptide 20 antibody
        • DEAD box protein 20 antibody
        • DEAD box protein DP 103 antibody
        • DEAD box protein DP103 antibody
        • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 103kD antibody
        • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 antibody
        • DEAD/H BOX 20 antibody
        • DKFZP434H052 antibody
        • DP 103 antibody
        • DP103 antibody
        • Gemin-3 antibody
        • Gemin3 antibody
        • Probable ATP dependent RNA helicase DDX20 antibody
        • Probable ATP-dependent RNA helicase ddx20 antibody
        • SMN interacting protein antibody
        see all

      Images

      • Flow Cytometry - Anti-Gemin 3 antibody [12H12] (ab10305)
        Flow Cytometry - Anti-Gemin 3 antibody [12H12] (ab10305)
        Overlay histogram showing HeLa cells stained with ab10305 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10305, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

      Protocols

      • Flow cytometry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (6)

      Publishing research using ab10305? Please let us know so that we can cite the reference in this datasheet.

      ab10305 has been referenced in 6 publications.

      • Mahmoudi S  et al. WRAP53 is essential for Cajal body formation and for targeting the survival of motor neuron complex to Cajal bodies. PLoS Biol 8:e1000521 (2010). WB ; Human . PubMed: 21072240
      • Bradrick SS & Gromeier M Identification of gemin5 as a novel 7-methylguanosine cap-binding protein. PLoS One 4:e7030 (2009). WB ; Human . PubMed: 19750007
      • Castelló A  et al. RNA nuclear export is blocked by poliovirus 2A protease and is concomitant with nucleoporin cleavage. J Cell Sci 122:3799-809 (2009). WB ; Human . PubMed: 19789179
      • Walker MP  et al. SMN complex localizes to the sarcomeric Z-disc and is a proteolytic target of calpain. Hum Mol Genet 17:3399-410 (2008). ICC/IF ; Hamster . PubMed: 18689355
      • Morency E  et al. A novel cell response triggered by interphase centromere structural instability. J Cell Biol 177:757-68 (2007). ICC/IF ; Human . PubMed: 17548509
      • Zhang H  et al. Multiprotein complexes of the survival of motor neuron protein SMN with Gemins traffic to neuronal processes and growth cones of motor neurons. J Neurosci 26:8622-32 (2006). PubMed: 16914688

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-3 of 3 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Gemin 3 antibody [12H12]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HEK293)
      Specification
      HEK293
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0.4% triton X100
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Aug 16 2012

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Gemin 3 antibody [12H12]

      Excellent
      Abreviews
      Abreviews
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HeLa)
      Specification
      HeLa
      Fixative
      Paraformaldehyde
      Blocking step
      Serum as blocking agent for 15 minute(s) · Concentration: 1%
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Feb 06 2006

      Question

      Customer would like to know which epitope ab10305 binds to.

      Read More

      Abcam community

      Verified customer

      Asked on Feb 20 2004

      Answer

      I'm afraid that we have no further information on the epitope for this antibody.

      Read More

      Abcam Scientific Support

      Answered on Feb 20 2004

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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