Anti-Gemin 3 antibody [12H12] (ab10305)
- Datasheet
- References (6)
- Protocols
Overview
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Product nameAnti-Gemin 3 antibody [12H12]
See all Gemin 3 primary antibodies -
DescriptionMouse monoclonal [12H12] to Gemin 3
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Host speciesMouse
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Tested applicationsSuitable for: ELISA, WB, IP, Flow Cyt, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant 6His-tag C-terminal domain of Gemin 3 (amino acids 368-548).
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General notes
The survival of motor neurons (SMN) gene is the disease gene of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The SMN protein is part of a complex containing several proteins, of which one, SIP1 (SMN interacting protein 1), has been characterized so far. The SMN complex is found in both the cytoplasm and in the nucleus, where it is concentrated in bodies called gems. In the cytoplasm, SMN and SIP1 interact with the Sm core proteins of spliceosomal small nuclear ribonucleoproteins (snRNPs), and they play a critical role in snRNP assembly. In the nucleus, SMN is required for pre-mRNA splicing, likely by serving in the regeneration of snRNPs. A DEAD box putative RNA helicase, named Gemin 3 which is another component of the SMN complex, has been identified. Gemin 3 interacts directly with SMN, as well as with SmB, SmD2 and SmD3. Immunolocalization studies using mAbs to Gemin 3 show that it colocalizes with SMN in gems. Gemin 3 binds SMN via its unique COOH-terminal domain, and SMN mutations found in some SMA patients strongly reduce this interaction. The presence of a DEAD box motif in Gemin 3 suggests that it may provide the catalytic activity that plays a critical role in the function of the SMN complex on RNPs.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityProtein A purified
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Purification notesProtein A purified from tissue culture supernatant.
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Primary antibody notesThe survival of motor neurons (SMN) gene is the disease gene of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The SMN protein is part of a complex containing several proteins, of which one, SIP1 (SMN interacting protein 1), has been characterized so far. The SMN complex is found in both the cytoplasm and in the nucleus, where it is concentrated in bodies called gems. In the cytoplasm, SMN and SIP1 interact with the Sm core proteins of spliceosomal small nuclear ribonucleoproteins (snRNPs), and they play a critical role in snRNP assembly. In the nucleus, SMN is required for pre-mRNA splicing, likely by serving in the regeneration of snRNPs. A DEAD box putative RNA helicase, named Gemin 3 which is another component of the SMN complex, has been identified. Gemin 3 interacts directly with SMN, as well as with SmB, SmD2 and SmD3. Immunolocalization studies using mAbs to Gemin 3 show that it colocalizes with SMN in gems. Gemin 3 binds SMN via its unique COOH-terminal domain, and SMN mutations found in some SMA patients strongly reduce this interaction. The presence of a DEAD box motif in Gemin 3 suggests that it may provide the catalytic activity that plays a critical role in the function of the SMN complex on RNPs.
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ClonalityMonoclonal
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Clone number12H12
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MyelomaSp2/0
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab10305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| ELISA | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 105 kDa (predicted molecular weight: 99 kDa). | |
| IP | Use at an assay dependent concentration. | |
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| ICC/IF | 1/50. |
Target
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FunctionThe SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. It may also play a role in the metabolism of snoRNPs.
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Tissue specificityUbiquitous.
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Sequence similaritiesBelongs to the DEAD box helicase family. DDX20 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localizationCytoplasm. Nucleus > gem. Localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies.
- Information by UniProt
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Database links
- Entrez Gene: 11218 Human
- Omim: 606168 Human
- SwissProt: Q9UHI6 Human
- Unigene: 591405 Human
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Alternative names
- Component of gems 3 antibody
- DDX 20 antibody
- ddx20 antibody
see all
Images
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![Flow Cytometry - Anti-Gemin 3 antibody [12H12] (ab10305)](https://www.abcam.com/ps/products/10/ab10305/Images/Gemin-3-Primary-antibodies-ab10305-1.jpg)
Flow Cytometry - Anti-Gemin 3 antibody [12H12] (ab10305)Overlay histogram showing HeLa cells stained with ab10305 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10305, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. -
![Immunocytochemistry/ Immunofluorescence - Anti-Gemin 3 antibody [12H12] (ab10305)](https://www.abcam.com/ps/products/10/ab10305/Images/Gemin-3-Primary-antibodies-ab10305-9.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Gemin 3 antibody [12H12] (ab10305)This image is courtesy of an anonymous Abreviewab10305 staining Gemin 3 in the Human HEK cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde, permeabilized with Triton X-100 0.4% and blocked with 3% serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/50 in PBS + 1% Goat serum) for 1 hour at 25°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.
Protocols
References
This product has been referenced in:
- Mahmoudi S et al. WRAP53 is essential for Cajal body formation and for targeting the survival of motor neuron complex to Cajal bodies. PLoS Biol 8:e1000521 (2010). WB ; Human . Read more (PubMed: 21072240) »
- Bradrick SS & Gromeier M Identification of gemin5 as a novel 7-methylguanosine cap-binding protein. PLoS One 4:e7030 (2009). WB ; Human . Read more (PubMed: 19750007) »
