Product nameAnti-Gemin 3 antibody
See all Gemin 3 primary antibodies
DescriptionRabbit polyclonal to Gemin 3
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residues 600 and 650 of human Gemin 3 (NP_009135.3).
- Whole cell lysate from HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 92 kDa).|
|IP||Use at 1-4 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionThe SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. It may also play a role in the metabolism of snoRNPs.
Sequence similaritiesBelongs to the DEAD box helicase family. DDX20 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Cellular localizationCytoplasm. Nucleus > gem. Localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies.
- Information by UniProt
- Component of gems 3 antibody
- DDX 20 antibody
- ddx20 antibody
All lanes : Anti-Gemin 3 antibody (ab70896) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 92 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa, 175 kDa, 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Gemin 3 with ab70896 at 1/200 (1µg/ml). Detection: DAB.
Detection of Human Gemin 3 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP), using ab70896 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
ab70896 has not yet been referenced specifically in any publications.