Key features and details
- Rabbit polyclonal to Gemin 4
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Gemin 4 antibody
DescriptionRabbit polyclonal to Gemin 4
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 1010 and the C-terminus (residue 1058) of Human Gemin 4 (NP_056536.1).
- Whole cell lysate from HeLa and 293T cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70750 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 120 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceGEMIN 4 is part of the SMN complex and plays an essential role in spliceosomal snRNP assembly in the cytoplasm. It is required for pre mRNA splicing in the nucleus.
Cellular localizationCytoplasmic and nuclear.
- Component of gems 4 antibody
- Gem associated protein 4 antibody
- HC56 antibody
- HHRF 1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Gemin 4 with ab70750 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-Gemin 4 antibody (ab70750) at 1/5000 dilution
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 120 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?
Additional bands at: 160 kDa, 270 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Detection of Gemin 4 by Western Blot of Immunprecipitate. ab70750 at 1µg/ml staining Gemin 4 in HeLa whole cell lysate immunoprecipitated using ab70750 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: chemiluminescence with exposure time of 30 seconds.
ab70750 has not yet been referenced specifically in any publications.