Overview

  • Product name

    Anti-Gemin 5 antibody - C-terminal
    See all Gemin 5 primary antibodies
  • Description

    Rabbit polyclonal to Gemin 5 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Gemin 5 (C terminal). The exact sequence is proprietary.
    Database link: Q8TEQ6

  • Positive control

    • ICC/IF: HeLa cells. IP: HepG2 whole cell extracts. WB: HeLa and HepG2 whole cell extracts.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IP 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 169 kDa.

Target

  • Relevance

    Gemin 5 is part of a large macromolecular complex localized to both the cytoplasm and the nucleus that plays a role in the cytoplasmic assembly of small nuclear ribonucleoproteins (snRNPs). Other members of this complex include SMN, Gemin 2 (SIP1), Gemin 3 (DDX20), and Gemin 4.
  • Cellular localization

    Nuclear and cytoplasmic
  • Database links

  • Alternative names

    • gem (nuclear organelle) associated protein 5 antibody
    • Gem-associated protein 5 antibody
    • GEMIN5 antibody

Images

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Gemin 5 (green) using ab229640 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue).

  • All lanes : Anti-Gemin 5 antibody - C-terminal (ab229640) at 1/500 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 169 kDa



    5% SDS-PAGE

  • Gemin 5 was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extracts using 5 µg of ab229640. Western blot was performed from the immunoprecipitate using ab229640.

    Lane 1: Control IgG IP in HepG2 whole cell extracts.

    Lane 2: ab229640 IP in HepG2 whole cell extracts.

References

ab229640 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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