Key features and details
- Rabbit polyclonal to Gemin 5 - C-terminal
- Suitable for: ICC/IF, IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Gemin 5 antibody - C-terminal
See all Gemin 5 primary antibodies
DescriptionRabbit polyclonal to Gemin 5 - C-terminal
Tested applicationsSuitable for: ICC/IF, IP, WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human Gemin 5 (C terminal). The exact sequence is proprietary.
Database link: Q8TEQ6
- ICC/IF: HeLa cells. IP: HepG2 whole cell extracts. WB: HeLa and HepG2 whole cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|IP||1/100 - 1/500.|
|WB||1/500 - 1/3000. Predicted molecular weight: 169 kDa.|
RelevanceGemin 5 is part of a large macromolecular complex localized to both the cytoplasm and the nucleus that plays a role in the cytoplasmic assembly of small nuclear ribonucleoproteins (snRNPs). Other members of this complex include SMN, Gemin 2 (SIP1), Gemin 3 (DDX20), and Gemin 4.
Cellular localizationNuclear and cytoplasmic
- gem (nuclear organelle) associated protein 5 antibody
- Gem-associated protein 5 antibody
- GEMIN5 antibody
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Gemin 5 (green) using ab229640 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue).
All lanes : Anti-Gemin 5 antibody - C-terminal (ab229640) at 1/500 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell extracts
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 169 kDa
Gemin 5 was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extracts using 5 µg of ab229640. Western blot was performed from the immunoprecipitate using ab229640.
Lane 1: Control IgG IP in HepG2 whole cell extracts.
Lane 2: ab229640 IP in HepG2 whole cell extracts.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229640 has not yet been referenced specifically in any publications.