Key features and details
- Rabbit polyclonal to Gemin 8
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Gemin 8 antibody
See all Gemin 8 primary antibodies
DescriptionRabbit polyclonal to Gemin 8
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
- IHC-P: Human thyroid gland tissue. WB: Human RT4 and U-251 MG sp cell lysates. ICC/IF: U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 29 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
RelevanceGemin 8 forms a large macromolecular complex which includes other gemin proteins, SMN (survival motor neuron) protein and Unrip. This complex is required for assembly of small nuclear ribonucleoproteins (snRNPs) from Sm proteins and snRNAs. Gemin 8 mediates the binding of SMN with gemin 6 and gemin 7.
Cellular localizationCytoplasmic and Nuclear
- FAM51A1 antibody
- FLJ20514 antibody
- FLJ43072 antibody
- gem (nuclear organelle) associated protein 8 antibody
Paraffin-embedded human thyroid gland tissue stained for Gemin 8 using ab224758 at 1/20 dilution in immunohistochemical analysis.
All lanes : Anti-Gemin 8 antibody (ab224758) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG sp (human brain glioma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 29 kDa
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Gemin 8 (green) using ab224758 at 4 µg/ml in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224758 has not yet been referenced specifically in any publications.