Overview

  • Product name

  • Description

    Mouse polyclonal to Gemin6/SIP2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human Gemin6/SIP2.
    Database link: AAH18195.1

  • Positive control

    • HeLa cell lysate with Gemin6/SIP2 expression, Gemin6/SIP2 transfected 293T cell lysate and HeLa cells.
  • General notes

    Previously labelled as Gemin 6. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab88290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 19 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus.
  • Cellular localization

    Nucleus > nucleoplasm. Nucleus > gem. Cytoplasm. Found both in the nucleoplasm and in nuclear bodies called gems (Gemini of Cajal bodies) that are often in proximity to Cajal (coiled) bodies. Also found in the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ23459 antibody
    • Gem (nuclear organelle) associated protein 6 antibody
    • Gem-associated protein 6 antibody
    • GEMI6 antibody
    • GEMI6_HUMAN antibody
    • Gemin 6 antibody
    • Gemin-6 antibody
    • Gemin6 antibody
    • SIP2 antibody
    see all

Images

  • Anti-Gemin6/SIP2 antibody (ab88290) at 1 µg/ml + HeLa cell lysate with Gemin6/SIP2 expression at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa

  • All lanes : Anti-Gemin6/SIP2 antibody (ab88290) at 1 µg/ml

    Lane 1 : Gemin6/SIP2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa

  • Immunofluorescence of ab88290 on HeLa cell with antibody concentration at 10ug/ml.

References

ab88290 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab88290.
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